Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4698 and Pf6N2E2_4699 are separated by 100 nucleotides Pf6N2E2_4699 and Pf6N2E2_4700 are separated by 234 nucleotides Pf6N2E2_4700 and Pf6N2E2_4701 are separated by 80 nucleotides Pf6N2E2_4701 and Pf6N2E2_4702 are separated by 15 nucleotides
Pf6N2E2_4698 - putative DNA binding protein, at 5,431,205 to 5,431,531
_4698
Pf6N2E2_4699 - FIG00953563: hypothetical protein, at 5,431,632 to 5,432,033
_4699
Pf6N2E2_4700 - Microsomal dipeptidase (EC 3.4.13.19), at 5,432,268 to 5,433,245
_4700
Pf6N2E2_4701 - FIG00953113: hypothetical protein, at 5,433,326 to 5,433,856
_4701
Pf6N2E2_4702 - DgcA Dimethylglycine demethylase subunit A, at 5,433,872 to 5,435,932
_4702
Position (kb)
5432
5433
5434 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.458 kb on + strand, within Pf6N2E2_4698 at 5431.511 kb on - strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.573 kb on + strand at 5431.576 kb on - strand at 5431.677 kb on + strand, within Pf6N2E2_4699 at 5431.678 kb on - strand, within Pf6N2E2_4699 at 5431.678 kb on - strand, within Pf6N2E2_4699 at 5431.678 kb on - strand, within Pf6N2E2_4699 at 5431.711 kb on + strand, within Pf6N2E2_4699 at 5431.711 kb on + strand, within Pf6N2E2_4699 at 5431.712 kb on - strand, within Pf6N2E2_4699 at 5431.712 kb on - strand, within Pf6N2E2_4699 at 5431.913 kb on - strand, within Pf6N2E2_4699 at 5432.127 kb on + strand at 5432.127 kb on + strand at 5432.127 kb on + strand at 5432.128 kb on - strand at 5432.203 kb on + strand at 5432.203 kb on + strand at 5432.203 kb on + strand at 5432.203 kb on + strand at 5432.203 kb on + strand at 5432.203 kb on + strand at 5432.204 kb on - strand at 5432.204 kb on - strand at 5432.204 kb on - strand at 5432.204 kb on - strand at 5432.204 kb on - strand at 5432.577 kb on + strand, within Pf6N2E2_4700 at 5432.625 kb on + strand, within Pf6N2E2_4700 at 5432.625 kb on + strand, within Pf6N2E2_4700 at 5432.658 kb on + strand, within Pf6N2E2_4700 at 5432.659 kb on - strand, within Pf6N2E2_4700 at 5432.799 kb on + strand, within Pf6N2E2_4700 at 5432.800 kb on - strand, within Pf6N2E2_4700 at 5432.968 kb on - strand, within Pf6N2E2_4700 at 5433.070 kb on - strand, within Pf6N2E2_4700 at 5433.270 kb on + strand at 5433.298 kb on + strand at 5433.352 kb on + strand at 5433.407 kb on + strand, within Pf6N2E2_4701 at 5433.407 kb on + strand, within Pf6N2E2_4701 at 5433.407 kb on + strand, within Pf6N2E2_4701 at 5433.408 kb on - strand, within Pf6N2E2_4701 at 5433.473 kb on + strand, within Pf6N2E2_4701 at 5433.489 kb on - strand, within Pf6N2E2_4701 at 5433.489 kb on - strand, within Pf6N2E2_4701 at 5433.575 kb on + strand, within Pf6N2E2_4701 at 5433.575 kb on + strand, within Pf6N2E2_4701 at 5433.575 kb on + strand, within Pf6N2E2_4701 at 5433.575 kb on + strand, within Pf6N2E2_4701 at 5433.575 kb on + strand, within Pf6N2E2_4701 at 5433.575 kb on + strand, within Pf6N2E2_4701 at 5433.576 kb on - strand, within Pf6N2E2_4701 at 5433.576 kb on - strand, within Pf6N2E2_4701 at 5433.576 kb on - strand, within Pf6N2E2_4701 at 5433.576 kb on - strand, within Pf6N2E2_4701 at 5433.577 kb on + strand, within Pf6N2E2_4701 at 5433.577 kb on + strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.578 kb on - strand, within Pf6N2E2_4701 at 5433.680 kb on + strand, within Pf6N2E2_4701 at 5433.680 kb on + strand, within Pf6N2E2_4701 at 5433.680 kb on + strand, within Pf6N2E2_4701 at 5433.681 kb on - strand, within Pf6N2E2_4701 at 5433.681 kb on - strand, within Pf6N2E2_4701 at 5433.681 kb on - strand, within Pf6N2E2_4701 at 5433.687 kb on - strand, within Pf6N2E2_4701 at 5433.687 kb on - strand, within Pf6N2E2_4701 at 5433.713 kb on + strand, within Pf6N2E2_4701 at 5433.713 kb on + strand, within Pf6N2E2_4701 at 5433.713 kb on + strand, within Pf6N2E2_4701 at 5433.713 kb on + strand, within Pf6N2E2_4701 at 5433.713 kb on + strand, within Pf6N2E2_4701 at 5433.714 kb on - strand, within Pf6N2E2_4701 at 5433.714 kb on - strand, within Pf6N2E2_4701 at 5433.800 kb on + strand, within Pf6N2E2_4701 at 5433.800 kb on + strand, within Pf6N2E2_4701 at 5433.854 kb on + strand at 5433.854 kb on + strand at 5433.959 kb on + strand at 5433.959 kb on + strand at 5433.959 kb on + strand at 5433.960 kb on - strand at 5434.001 kb on + strand at 5434.001 kb on + strand at 5434.002 kb on - strand at 5434.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 5,431,449 + Pf6N2E2_4698 0.75 -2.1 5,431,449 + Pf6N2E2_4698 0.75 -0.2 5,431,449 + Pf6N2E2_4698 0.75 -0.4 5,431,449 + Pf6N2E2_4698 0.75 +1.1 5,431,449 + Pf6N2E2_4698 0.75 -0.3 5,431,449 + Pf6N2E2_4698 0.75 -0.3 5,431,449 + Pf6N2E2_4698 0.75 -0.9 5,431,449 + Pf6N2E2_4698 0.75 -1.5 5,431,449 + Pf6N2E2_4698 0.75 -1.1 5,431,449 + Pf6N2E2_4698 0.75 +0.2 5,431,449 + Pf6N2E2_4698 0.75 -0.9 5,431,450 - Pf6N2E2_4698 0.75 +0.2 5,431,450 - Pf6N2E2_4698 0.75 +0.2 5,431,450 - Pf6N2E2_4698 0.75 +0.6 5,431,450 - Pf6N2E2_4698 0.75 +0.2 5,431,450 - Pf6N2E2_4698 0.75 -1.3 5,431,458 + Pf6N2E2_4698 0.77 -0.6 5,431,511 - -2.8 5,431,516 + +0.5 5,431,516 + +0.2 5,431,516 + +0.3 5,431,516 + +0.1 5,431,516 + -0.7 5,431,516 + +0.4 5,431,517 - +0.2 5,431,517 - +0.4 5,431,517 - +0.6 5,431,517 - -0.8 5,431,517 - +1.0 5,431,517 - +0.3 5,431,517 - -0.4 5,431,517 - -1.5 5,431,517 - +0.5 5,431,517 - -0.5 5,431,573 + +0.3 5,431,576 - +0.4 5,431,677 + Pf6N2E2_4699 0.11 -0.2 5,431,678 - Pf6N2E2_4699 0.11 +0.3 5,431,678 - Pf6N2E2_4699 0.11 +1.5 5,431,678 - Pf6N2E2_4699 0.11 +0.1 5,431,711 + Pf6N2E2_4699 0.20 +0.1 5,431,711 + Pf6N2E2_4699 0.20 -0.0 5,431,712 - Pf6N2E2_4699 0.20 -0.0 5,431,712 - Pf6N2E2_4699 0.20 +0.6 5,431,913 - Pf6N2E2_4699 0.70 -0.2 5,432,127 + -0.7 5,432,127 + -0.1 5,432,127 + -0.9 5,432,128 - +0.4 5,432,203 + +0.6 5,432,203 + +0.5 5,432,203 + -0.2 5,432,203 + +0.9 5,432,203 + +0.5 5,432,203 + -0.3 5,432,204 - -0.3 5,432,204 - -0.0 5,432,204 - -0.1 5,432,204 - -1.2 5,432,204 - +0.1 5,432,577 + Pf6N2E2_4700 0.32 -1.3 5,432,625 + Pf6N2E2_4700 0.37 +1.0 5,432,625 + Pf6N2E2_4700 0.37 -0.9 5,432,658 + Pf6N2E2_4700 0.40 -1.4 5,432,659 - Pf6N2E2_4700 0.40 -2.2 5,432,799 + Pf6N2E2_4700 0.54 -0.9 5,432,800 - Pf6N2E2_4700 0.54 -0.9 5,432,968 - Pf6N2E2_4700 0.72 -0.2 5,433,070 - Pf6N2E2_4700 0.82 -0.5 5,433,270 + -0.3 5,433,298 + -0.8 5,433,352 + +0.9 5,433,407 + Pf6N2E2_4701 0.15 -0.8 5,433,407 + Pf6N2E2_4701 0.15 -0.6 5,433,407 + Pf6N2E2_4701 0.15 +0.5 5,433,408 - Pf6N2E2_4701 0.15 +0.6 5,433,473 + Pf6N2E2_4701 0.28 -0.2 5,433,489 - Pf6N2E2_4701 0.31 -0.7 5,433,489 - Pf6N2E2_4701 0.31 -1.4 5,433,575 + Pf6N2E2_4701 0.47 +0.0 5,433,575 + Pf6N2E2_4701 0.47 -1.8 5,433,575 + Pf6N2E2_4701 0.47 -0.4 5,433,575 + Pf6N2E2_4701 0.47 -0.7 5,433,575 + Pf6N2E2_4701 0.47 +0.8 5,433,575 + Pf6N2E2_4701 0.47 -2.1 5,433,576 - Pf6N2E2_4701 0.47 +0.7 5,433,576 - Pf6N2E2_4701 0.47 -0.5 5,433,576 - Pf6N2E2_4701 0.47 -1.4 5,433,576 - Pf6N2E2_4701 0.47 -1.9 5,433,577 + Pf6N2E2_4701 0.47 +0.1 5,433,577 + Pf6N2E2_4701 0.47 +0.8 5,433,578 - Pf6N2E2_4701 0.47 -2.0 5,433,578 - Pf6N2E2_4701 0.47 +0.6 5,433,578 - Pf6N2E2_4701 0.47 +0.8 5,433,578 - Pf6N2E2_4701 0.47 +0.1 5,433,578 - Pf6N2E2_4701 0.47 -1.4 5,433,578 - Pf6N2E2_4701 0.47 -2.0 5,433,578 - Pf6N2E2_4701 0.47 +0.3 5,433,680 + Pf6N2E2_4701 0.67 +0.6 5,433,680 + Pf6N2E2_4701 0.67 -0.4 5,433,680 + Pf6N2E2_4701 0.67 -0.5 5,433,681 - Pf6N2E2_4701 0.67 +0.5 5,433,681 - Pf6N2E2_4701 0.67 -0.2 5,433,681 - Pf6N2E2_4701 0.67 +0.4 5,433,687 - Pf6N2E2_4701 0.68 -0.5 5,433,687 - Pf6N2E2_4701 0.68 -0.5 5,433,713 + Pf6N2E2_4701 0.73 +0.0 5,433,713 + Pf6N2E2_4701 0.73 +0.7 5,433,713 + Pf6N2E2_4701 0.73 -0.3 5,433,713 + Pf6N2E2_4701 0.73 -0.3 5,433,713 + Pf6N2E2_4701 0.73 +0.3 5,433,714 - Pf6N2E2_4701 0.73 -1.5 5,433,714 - Pf6N2E2_4701 0.73 -1.4 5,433,800 + Pf6N2E2_4701 0.89 -0.1 5,433,800 + Pf6N2E2_4701 0.89 +0.9 5,433,854 + -0.3 5,433,854 + +0.4 5,433,959 + -0.9 5,433,959 + +0.3 5,433,959 + +0.8 5,433,960 - -0.3 5,434,001 + -1.9 5,434,001 + -0.2 5,434,002 - -1.1 5,434,002 - -0.3
Or see this region's nucleotide sequence