Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3554 and Pf6N2E2_3555 are separated by 5 nucleotides Pf6N2E2_3555 and Pf6N2E2_3556 overlap by 4 nucleotides Pf6N2E2_3556 and Pf6N2E2_3557 are separated by 199 nucleotides Pf6N2E2_3557 and Pf6N2E2_3558 are separated by 246 nucleotides
Pf6N2E2_3554 - GCN5-related N-acetyltransferase, at 4,145,190 to 4,145,723
_3554
Pf6N2E2_3555 - Tabtoxin resistance protein, at 4,145,729 to 4,146,262
_3555
Pf6N2E2_3556 - Urease (EC 3.5.1.5) (from data) , at 4,146,259 to 4,148,409
_3556
Pf6N2E2_3557 - Chaperone-modulator protein CbpM, at 4,148,609 to 4,148,908
_3557
Pf6N2E2_3558 - DnaJ-class molecular chaperone CbpA, at 4,149,155 to 4,150,096
_3558
Position (kb)
4146
4147
4148
4149 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4145.454 kb on + strand, within Pf6N2E2_3554 at 4145.454 kb on + strand, within Pf6N2E2_3554 at 4145.455 kb on - strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.458 kb on + strand, within Pf6N2E2_3554 at 4145.459 kb on - strand, within Pf6N2E2_3554 at 4145.459 kb on - strand, within Pf6N2E2_3554 at 4145.459 kb on - strand, within Pf6N2E2_3554 at 4145.728 kb on + strand at 4145.728 kb on + strand at 4145.728 kb on + strand at 4145.728 kb on + strand at 4145.729 kb on - strand at 4145.873 kb on + strand, within Pf6N2E2_3555 at 4145.873 kb on + strand, within Pf6N2E2_3555 at 4145.873 kb on + strand, within Pf6N2E2_3555 at 4146.016 kb on - strand, within Pf6N2E2_3555 at 4146.016 kb on - strand, within Pf6N2E2_3555 at 4146.016 kb on - strand, within Pf6N2E2_3555 at 4146.016 kb on - strand, within Pf6N2E2_3555 at 4146.110 kb on + strand, within Pf6N2E2_3555 at 4146.110 kb on - strand, within Pf6N2E2_3555 at 4146.111 kb on - strand, within Pf6N2E2_3555 at 4146.111 kb on - strand, within Pf6N2E2_3555 at 4146.150 kb on - strand, within Pf6N2E2_3555 at 4146.165 kb on - strand, within Pf6N2E2_3555 at 4146.216 kb on - strand at 4146.233 kb on + strand at 4146.233 kb on + strand at 4146.233 kb on + strand at 4146.234 kb on - strand at 4146.234 kb on - strand at 4146.234 kb on - strand at 4146.237 kb on - strand at 4146.237 kb on - strand at 4146.324 kb on + strand at 4146.325 kb on - strand at 4146.730 kb on + strand, within Pf6N2E2_3556 at 4146.731 kb on - strand, within Pf6N2E2_3556 at 4146.820 kb on + strand, within Pf6N2E2_3556 at 4147.420 kb on + strand, within Pf6N2E2_3556 at 4147.516 kb on + strand, within Pf6N2E2_3556 at 4147.516 kb on + strand, within Pf6N2E2_3556 at 4147.516 kb on + strand, within Pf6N2E2_3556 at 4147.517 kb on - strand, within Pf6N2E2_3556 at 4147.583 kb on + strand, within Pf6N2E2_3556 at 4147.583 kb on + strand, within Pf6N2E2_3556 at 4147.583 kb on + strand, within Pf6N2E2_3556 at 4147.583 kb on + strand, within Pf6N2E2_3556 at 4147.584 kb on - strand, within Pf6N2E2_3556 at 4147.584 kb on - strand, within Pf6N2E2_3556 at 4147.584 kb on - strand, within Pf6N2E2_3556 at 4147.584 kb on - strand, within Pf6N2E2_3556 at 4147.912 kb on + strand, within Pf6N2E2_3556 at 4147.912 kb on + strand, within Pf6N2E2_3556 at 4147.912 kb on + strand, within Pf6N2E2_3556 at 4147.912 kb on + strand, within Pf6N2E2_3556 at 4147.913 kb on - strand, within Pf6N2E2_3556 at 4147.924 kb on + strand, within Pf6N2E2_3556 at 4147.924 kb on + strand, within Pf6N2E2_3556 at 4147.924 kb on + strand, within Pf6N2E2_3556 at 4147.924 kb on + strand, within Pf6N2E2_3556 at 4147.924 kb on + strand, within Pf6N2E2_3556 at 4147.925 kb on - strand, within Pf6N2E2_3556 at 4147.925 kb on - strand, within Pf6N2E2_3556 at 4147.925 kb on - strand, within Pf6N2E2_3556 at 4147.925 kb on - strand, within Pf6N2E2_3556 at 4147.925 kb on - strand, within Pf6N2E2_3556 at 4147.925 kb on - strand, within Pf6N2E2_3556 at 4148.293 kb on + strand at 4148.294 kb on - strand at 4148.294 kb on - strand at 4148.326 kb on + strand at 4148.326 kb on + strand at 4148.326 kb on + strand at 4148.326 kb on + strand at 4148.327 kb on - strand at 4148.327 kb on - strand at 4148.327 kb on - strand at 4148.327 kb on - strand at 4148.395 kb on + strand at 4148.550 kb on + strand at 4148.550 kb on + strand at 4148.551 kb on - strand at 4148.551 kb on - strand at 4148.551 kb on - strand at 4148.551 kb on - strand at 4148.551 kb on - strand at 4148.580 kb on + strand at 4148.580 kb on + strand at 4148.581 kb on - strand at 4148.581 kb on - strand at 4148.581 kb on - strand at 4148.610 kb on + strand at 4148.611 kb on - strand at 4148.611 kb on - strand at 4148.611 kb on - strand at 4148.766 kb on + strand, within Pf6N2E2_3557 at 4148.767 kb on - strand, within Pf6N2E2_3557 at 4148.931 kb on - strand at 4148.990 kb on + strand at 4148.991 kb on - strand at 4149.091 kb on + strand at 4149.091 kb on + strand at 4149.091 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 4,145,454 + Pf6N2E2_3554 0.49 -0.6 4,145,454 + Pf6N2E2_3554 0.49 -0.2 4,145,455 - Pf6N2E2_3554 0.50 -0.5 4,145,458 + Pf6N2E2_3554 0.50 -1.7 4,145,458 + Pf6N2E2_3554 0.50 +1.1 4,145,458 + Pf6N2E2_3554 0.50 +0.3 4,145,458 + Pf6N2E2_3554 0.50 +0.0 4,145,458 + Pf6N2E2_3554 0.50 +0.1 4,145,458 + Pf6N2E2_3554 0.50 +1.2 4,145,458 + Pf6N2E2_3554 0.50 +0.6 4,145,458 + Pf6N2E2_3554 0.50 +0.5 4,145,458 + Pf6N2E2_3554 0.50 +0.6 4,145,459 - Pf6N2E2_3554 0.50 -0.5 4,145,459 - Pf6N2E2_3554 0.50 -1.3 4,145,459 - Pf6N2E2_3554 0.50 +0.1 4,145,728 + -0.5 4,145,728 + -0.4 4,145,728 + -0.1 4,145,728 + -0.8 4,145,729 - -0.6 4,145,873 + Pf6N2E2_3555 0.27 +0.9 4,145,873 + Pf6N2E2_3555 0.27 -0.7 4,145,873 + Pf6N2E2_3555 0.27 -0.3 4,146,016 - Pf6N2E2_3555 0.54 -0.6 4,146,016 - Pf6N2E2_3555 0.54 -0.9 4,146,016 - Pf6N2E2_3555 0.54 +1.4 4,146,016 - Pf6N2E2_3555 0.54 -0.5 4,146,110 + Pf6N2E2_3555 0.71 +0.3 4,146,110 - Pf6N2E2_3555 0.71 +0.5 4,146,111 - Pf6N2E2_3555 0.72 -0.2 4,146,111 - Pf6N2E2_3555 0.72 -0.3 4,146,150 - Pf6N2E2_3555 0.79 -0.2 4,146,165 - Pf6N2E2_3555 0.82 -0.2 4,146,216 - +0.6 4,146,233 + +0.2 4,146,233 + +1.2 4,146,233 + +1.6 4,146,234 - +0.3 4,146,234 - +0.1 4,146,234 - -0.4 4,146,237 - +0.1 4,146,237 - -2.5 4,146,324 + -0.3 4,146,325 - -0.7 4,146,730 + Pf6N2E2_3556 0.22 -0.3 4,146,731 - Pf6N2E2_3556 0.22 -0.7 4,146,820 + Pf6N2E2_3556 0.26 +0.5 4,147,420 + Pf6N2E2_3556 0.54 +0.4 4,147,516 + Pf6N2E2_3556 0.58 -0.7 4,147,516 + Pf6N2E2_3556 0.58 -0.7 4,147,516 + Pf6N2E2_3556 0.58 +0.8 4,147,517 - Pf6N2E2_3556 0.58 -0.0 4,147,583 + Pf6N2E2_3556 0.62 -0.4 4,147,583 + Pf6N2E2_3556 0.62 +0.7 4,147,583 + Pf6N2E2_3556 0.62 -0.2 4,147,583 + Pf6N2E2_3556 0.62 +1.2 4,147,584 - Pf6N2E2_3556 0.62 -0.5 4,147,584 - Pf6N2E2_3556 0.62 +1.4 4,147,584 - Pf6N2E2_3556 0.62 +1.1 4,147,584 - Pf6N2E2_3556 0.62 +0.6 4,147,912 + Pf6N2E2_3556 0.77 +0.3 4,147,912 + Pf6N2E2_3556 0.77 -0.0 4,147,912 + Pf6N2E2_3556 0.77 +1.1 4,147,912 + Pf6N2E2_3556 0.77 -1.0 4,147,913 - Pf6N2E2_3556 0.77 +1.3 4,147,924 + Pf6N2E2_3556 0.77 +1.0 4,147,924 + Pf6N2E2_3556 0.77 -0.1 4,147,924 + Pf6N2E2_3556 0.77 -0.2 4,147,924 + Pf6N2E2_3556 0.77 +0.4 4,147,924 + Pf6N2E2_3556 0.77 -1.4 4,147,925 - Pf6N2E2_3556 0.77 -1.1 4,147,925 - Pf6N2E2_3556 0.77 +0.1 4,147,925 - Pf6N2E2_3556 0.77 +0.8 4,147,925 - Pf6N2E2_3556 0.77 -1.0 4,147,925 - Pf6N2E2_3556 0.77 +0.7 4,147,925 - Pf6N2E2_3556 0.77 -0.0 4,148,293 + +0.1 4,148,294 - -0.8 4,148,294 - +0.0 4,148,326 + +1.0 4,148,326 + +1.1 4,148,326 + +0.2 4,148,326 + +0.4 4,148,327 - +1.7 4,148,327 - +2.3 4,148,327 - -1.4 4,148,327 - -0.6 4,148,395 + +0.8 4,148,550 + +0.5 4,148,550 + -0.3 4,148,551 - +0.9 4,148,551 - -0.1 4,148,551 - +0.1 4,148,551 - -0.5 4,148,551 - -0.1 4,148,580 + -0.4 4,148,580 + +1.3 4,148,581 - +0.4 4,148,581 - +1.7 4,148,581 - +0.1 4,148,610 + -0.2 4,148,611 - -0.2 4,148,611 - +0.1 4,148,611 - -0.0 4,148,766 + Pf6N2E2_3557 0.52 +0.1 4,148,767 - Pf6N2E2_3557 0.53 -1.5 4,148,931 - +0.7 4,148,990 + -0.2 4,148,991 - -0.7 4,149,091 + +0.9 4,149,091 + -0.4 4,149,091 + +0.7
Or see this region's nucleotide sequence