Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3427 and Pf6N2E2_3428 are separated by 50 nucleotides Pf6N2E2_3428 and Pf6N2E2_3429 are separated by 214 nucleotides Pf6N2E2_3429 and Pf6N2E2_3430 are separated by 12 nucleotides
Pf6N2E2_3427 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2); Putative hemin-binding lipoprotein, at 3,983,945 to 3,985,459
_3427
Pf6N2E2_3428 - hypothetical protein, at 3,985,510 to 3,986,100
_3428
Pf6N2E2_3429 - hypothetical protein, at 3,986,315 to 3,986,443
_3429
Pf6N2E2_3430 - Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7), at 3,986,456 to 3,988,720
_3430
Position (kb)
3986
3987 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3985.361 kb on + strand at 3985.361 kb on + strand at 3985.361 kb on + strand at 3985.364 kb on + strand at 3985.364 kb on + strand at 3985.365 kb on - strand at 3985.365 kb on - strand at 3985.365 kb on - strand at 3985.373 kb on + strand at 3985.381 kb on + strand at 3985.381 kb on + strand at 3985.382 kb on - strand at 3985.382 kb on - strand at 3985.382 kb on - strand at 3985.382 kb on - strand at 3985.488 kb on - strand at 3985.922 kb on + strand, within Pf6N2E2_3428 at 3985.922 kb on + strand, within Pf6N2E2_3428 at 3985.922 kb on + strand, within Pf6N2E2_3428 at 3985.923 kb on - strand, within Pf6N2E2_3428 at 3985.950 kb on - strand, within Pf6N2E2_3428 at 3986.005 kb on - strand, within Pf6N2E2_3428 at 3986.024 kb on - strand, within Pf6N2E2_3428 at 3986.024 kb on - strand, within Pf6N2E2_3428 at 3986.131 kb on + strand at 3986.131 kb on + strand at 3986.132 kb on - strand at 3986.132 kb on - strand at 3986.160 kb on - strand at 3986.195 kb on + strand at 3986.196 kb on - strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.198 kb on + strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.199 kb on - strand at 3986.203 kb on - strand at 3986.203 kb on - strand at 3986.220 kb on + strand at 3986.221 kb on - strand at 3986.221 kb on - strand at 3986.241 kb on + strand at 3986.242 kb on - strand at 3986.271 kb on - strand at 3986.271 kb on - strand at 3986.289 kb on + strand at 3986.289 kb on + strand at 3986.323 kb on + strand at 3986.324 kb on - strand at 3986.324 kb on - strand at 3986.324 kb on - strand at 3986.324 kb on - strand at 3986.327 kb on + strand at 3986.328 kb on - strand, within Pf6N2E2_3429 at 3986.328 kb on - strand, within Pf6N2E2_3429 at 3986.384 kb on + strand, within Pf6N2E2_3429 at 3986.384 kb on + strand, within Pf6N2E2_3429 at 3986.385 kb on - strand, within Pf6N2E2_3429 at 3986.436 kb on - strand at 3986.447 kb on + strand at 3986.447 kb on + strand at 3986.448 kb on - strand at 3986.448 kb on - strand at 3986.448 kb on - strand at 3986.448 kb on - strand at 3986.448 kb on - strand at 3986.448 kb on - strand at 3986.700 kb on - strand, within Pf6N2E2_3430 at 3986.744 kb on + strand, within Pf6N2E2_3430 at 3986.744 kb on + strand, within Pf6N2E2_3430 at 3986.870 kb on + strand, within Pf6N2E2_3430 at 3986.870 kb on + strand, within Pf6N2E2_3430 at 3986.870 kb on + strand, within Pf6N2E2_3430 at 3986.871 kb on - strand, within Pf6N2E2_3430 at 3986.871 kb on - strand, within Pf6N2E2_3430 at 3986.871 kb on - strand, within Pf6N2E2_3430 at 3986.871 kb on - strand, within Pf6N2E2_3430 at 3987.000 kb on - strand, within Pf6N2E2_3430 at 3987.000 kb on - strand, within Pf6N2E2_3430 at 3987.000 kb on - strand, within Pf6N2E2_3430 at 3987.179 kb on + strand, within Pf6N2E2_3430 at 3987.179 kb on + strand, within Pf6N2E2_3430 at 3987.180 kb on - strand, within Pf6N2E2_3430
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 3,985,361 + +1.6 3,985,361 + +0.5 3,985,361 + +1.9 3,985,364 + +0.7 3,985,364 + +0.4 3,985,365 - +1.1 3,985,365 - +1.0 3,985,365 - +0.1 3,985,373 + +0.2 3,985,381 + -0.5 3,985,381 + +0.1 3,985,382 - +1.1 3,985,382 - -0.5 3,985,382 - -0.1 3,985,382 - -0.4 3,985,488 - -0.3 3,985,922 + Pf6N2E2_3428 0.70 -0.4 3,985,922 + Pf6N2E2_3428 0.70 +0.1 3,985,922 + Pf6N2E2_3428 0.70 -1.3 3,985,923 - Pf6N2E2_3428 0.70 +0.6 3,985,950 - Pf6N2E2_3428 0.74 +0.7 3,986,005 - Pf6N2E2_3428 0.84 -0.3 3,986,024 - Pf6N2E2_3428 0.87 -1.6 3,986,024 - Pf6N2E2_3428 0.87 -0.2 3,986,131 + +0.8 3,986,131 + +1.0 3,986,132 - -2.3 3,986,132 - -0.0 3,986,160 - -0.2 3,986,195 + +0.5 3,986,196 - -0.6 3,986,198 + +0.6 3,986,198 + +0.3 3,986,198 + +2.4 3,986,198 + +0.4 3,986,198 + -0.5 3,986,198 + -0.3 3,986,198 + +0.0 3,986,198 + -0.4 3,986,199 - -0.0 3,986,199 - -0.6 3,986,199 - +0.0 3,986,199 - -0.2 3,986,199 - -0.6 3,986,199 - -1.4 3,986,199 - +0.2 3,986,199 - -0.2 3,986,199 - -0.3 3,986,203 - -0.0 3,986,203 - +0.6 3,986,220 + +0.0 3,986,221 - +1.3 3,986,221 - -0.4 3,986,241 + +0.0 3,986,242 - -0.3 3,986,271 - +1.2 3,986,271 - -0.6 3,986,289 + +0.5 3,986,289 + +0.5 3,986,323 + +0.0 3,986,324 - -0.4 3,986,324 - +1.2 3,986,324 - +1.6 3,986,324 - -0.0 3,986,327 + -0.1 3,986,328 - Pf6N2E2_3429 0.10 -0.2 3,986,328 - Pf6N2E2_3429 0.10 -2.2 3,986,384 + Pf6N2E2_3429 0.53 -0.9 3,986,384 + Pf6N2E2_3429 0.53 -1.0 3,986,385 - Pf6N2E2_3429 0.54 -0.9 3,986,436 - -0.3 3,986,447 + -0.1 3,986,447 + +0.2 3,986,448 - +0.1 3,986,448 - -0.6 3,986,448 - +0.0 3,986,448 - +0.4 3,986,448 - -1.4 3,986,448 - -0.4 3,986,700 - Pf6N2E2_3430 0.11 +0.4 3,986,744 + Pf6N2E2_3430 0.13 -1.6 3,986,744 + Pf6N2E2_3430 0.13 +0.5 3,986,870 + Pf6N2E2_3430 0.18 -0.3 3,986,870 + Pf6N2E2_3430 0.18 +0.4 3,986,870 + Pf6N2E2_3430 0.18 -0.0 3,986,871 - Pf6N2E2_3430 0.18 -0.7 3,986,871 - Pf6N2E2_3430 0.18 +0.1 3,986,871 - Pf6N2E2_3430 0.18 +1.2 3,986,871 - Pf6N2E2_3430 0.18 +0.2 3,987,000 - Pf6N2E2_3430 0.24 +0.1 3,987,000 - Pf6N2E2_3430 0.24 -0.1 3,987,000 - Pf6N2E2_3430 0.24 +0.1 3,987,179 + Pf6N2E2_3430 0.32 -0.9 3,987,179 + Pf6N2E2_3430 0.32 -0.1 3,987,180 - Pf6N2E2_3430 0.32 -1.1
Or see this region's nucleotide sequence