Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_129 and Pf6N2E2_130 are separated by 65 nucleotides Pf6N2E2_130 and Pf6N2E2_131 are separated by 31 nucleotides Pf6N2E2_131 and Pf6N2E2_132 are separated by 219 nucleotides Pf6N2E2_132 and Pf6N2E2_133 are separated by 42 nucleotides Pf6N2E2_133 and Pf6N2E2_134 are separated by 342 nucleotides
Pf6N2E2_129 - AttH component of AttEFGH ABC transport system, at 179,571 to 180,644
_129
Pf6N2E2_130 - hypothetical protein, at 180,710 to 180,952
_130
Pf6N2E2_131 - hypothetical protein, at 180,984 to 181,157
_131
Pf6N2E2_132 - hcp, at 181,377 to 181,658
_132
Pf6N2E2_133 - hypothetical protein, at 181,701 to 181,850
_133
Pf6N2E2_134 - FIG00960493: hypothetical protein, at 182,193 to 183,569
_134
Position (kb) 181
182 Strain fitness (log2 ratio) -1
0
1
2 at 180.680 kb on + strand at 180.680 kb on + strand at 180.680 kb on + strand at 180.680 kb on + strand at 180.680 kb on + strand at 180.681 kb on - strand at 180.681 kb on - strand at 180.681 kb on - strand at 180.685 kb on + strand at 180.686 kb on - strand at 180.773 kb on + strand, within Pf6N2E2_130 at 181.179 kb on - strand at 181.186 kb on + strand at 181.205 kb on - strand at 181.221 kb on + strand at 181.221 kb on + strand at 181.221 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.255 kb on + strand at 181.256 kb on - strand at 181.312 kb on - strand at 181.321 kb on + strand at 181.321 kb on + strand at 181.428 kb on + strand, within Pf6N2E2_132 at 181.428 kb on + strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.519 kb on + strand, within Pf6N2E2_132 at 181.519 kb on + strand, within Pf6N2E2_132 at 181.564 kb on + strand, within Pf6N2E2_132 at 181.565 kb on - strand, within Pf6N2E2_132 at 181.565 kb on - strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.683 kb on - strand at 181.692 kb on + strand at 181.693 kb on - strand at 181.693 kb on - strand at 181.705 kb on - strand at 181.705 kb on - strand at 181.790 kb on + strand, within Pf6N2E2_133 at 181.811 kb on + strand, within Pf6N2E2_133 at 181.829 kb on + strand, within Pf6N2E2_133 at 181.851 kb on + strand at 181.851 kb on + strand at 181.851 kb on + strand at 181.851 kb on + strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.879 kb on + strand at 181.879 kb on + strand at 181.879 kb on + strand at 181.880 kb on - strand at 182.188 kb on + strand at 182.320 kb on + strand at 182.320 kb on + strand at 182.392 kb on + strand, within Pf6N2E2_134 at 182.392 kb on + strand, within Pf6N2E2_134 at 182.393 kb on - strand, within Pf6N2E2_134 at 182.393 kb on - strand, within Pf6N2E2_134 at 182.416 kb on + strand, within Pf6N2E2_134 at 182.416 kb on + strand, within Pf6N2E2_134 at 182.417 kb on - strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.581 kb on + strand, within Pf6N2E2_134 at 182.582 kb on - strand, within Pf6N2E2_134 at 182.582 kb on - strand, within Pf6N2E2_134
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 180,680 + -0.6 180,680 + -0.4 180,680 + -0.6 180,680 + -0.6 180,680 + -1.0 180,681 - +0.2 180,681 - -0.4 180,681 - +0.6 180,685 + +0.8 180,686 - -0.3 180,773 + Pf6N2E2_130 0.26 -0.4 181,179 - +1.6 181,186 + +0.2 181,205 - -0.8 181,221 + -0.5 181,221 + -0.2 181,221 + -0.1 181,224 + +0.2 181,224 + +0.7 181,224 + +0.4 181,224 + -0.4 181,224 + +0.2 181,224 + -0.8 181,224 + -0.1 181,224 + -0.3 181,225 - +0.6 181,225 - +1.0 181,225 - +0.0 181,225 - +1.6 181,255 + +0.0 181,256 - +2.6 181,312 - +0.0 181,321 + -1.0 181,321 + +0.5 181,428 + Pf6N2E2_132 0.18 -0.2 181,428 + Pf6N2E2_132 0.18 +1.2 181,429 - Pf6N2E2_132 0.18 +0.4 181,429 - Pf6N2E2_132 0.18 -0.0 181,429 - Pf6N2E2_132 0.18 +0.5 181,519 + Pf6N2E2_132 0.50 +0.6 181,519 + Pf6N2E2_132 0.50 +0.8 181,564 + Pf6N2E2_132 0.66 +1.6 181,565 - Pf6N2E2_132 0.67 +0.3 181,565 - Pf6N2E2_132 0.67 +1.5 181,567 + Pf6N2E2_132 0.67 +0.0 181,567 + Pf6N2E2_132 0.67 -0.5 181,567 + Pf6N2E2_132 0.67 +1.0 181,568 - Pf6N2E2_132 0.68 +0.6 181,568 - Pf6N2E2_132 0.68 -1.2 181,568 - Pf6N2E2_132 0.68 -0.4 181,683 - +0.1 181,692 + -1.4 181,693 - +1.2 181,693 - +1.1 181,705 - +0.3 181,705 - +0.1 181,790 + Pf6N2E2_133 0.59 -0.3 181,811 + Pf6N2E2_133 0.73 -0.3 181,829 + Pf6N2E2_133 0.85 -1.2 181,851 + -0.0 181,851 + +0.3 181,851 + +0.3 181,851 + +0.5 181,852 - +0.1 181,852 - +0.4 181,852 - +0.1 181,852 - -0.1 181,852 - +0.6 181,879 + -0.8 181,879 + -1.3 181,879 + -0.1 181,880 - -0.6 182,188 + -1.2 182,320 + +1.2 182,320 + -0.5 182,392 + Pf6N2E2_134 0.14 -0.1 182,392 + Pf6N2E2_134 0.14 -0.5 182,393 - Pf6N2E2_134 0.15 -0.8 182,393 - Pf6N2E2_134 0.15 +0.6 182,416 + Pf6N2E2_134 0.16 +0.8 182,416 + Pf6N2E2_134 0.16 +0.3 182,417 - Pf6N2E2_134 0.16 +0.7 182,506 + Pf6N2E2_134 0.23 +0.4 182,506 + Pf6N2E2_134 0.23 -0.2 182,506 + Pf6N2E2_134 0.23 -0.7 182,506 + Pf6N2E2_134 0.23 -0.1 182,581 + Pf6N2E2_134 0.28 +0.9 182,582 - Pf6N2E2_134 0.28 -1.6 182,582 - Pf6N2E2_134 0.28 +0.6
Or see this region's nucleotide sequence