Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_17930 and AO356_17935 are separated by 137 nucleotides AO356_17935 and AO356_17940 overlap by 4 nucleotides AO356_17940 and AO356_17945 are separated by 101 nucleotides AO356_17945 and AO356_17950 are separated by 2 nucleotides
AO356_17930: AO356_17930 - ornithine acetyltransferase, at 4,047,898 to 4,049,115
_17930
AO356_17935: AO356_17935 - glutathione S-transferase, at 4,049,253 to 4,049,885
_17935
AO356_17940: AO356_17940 - hypothetical protein, at 4,049,882 to 4,050,826
_17940
AO356_17945: AO356_17945 - cobalamin adenosyltransferase, at 4,050,928 to 4,051,509
_17945
AO356_17950: AO356_17950 - histidine kinase, at 4,051,512 to 4,053,548
_17950
Position (kb)
4049
4050
4051 Strain fitness (log2 ratio)
-2
-1
0
1 at 4048.969 kb on + strand, within AO356_17930 at 4048.969 kb on + strand, within AO356_17930 at 4048.969 kb on + strand, within AO356_17930 at 4048.969 kb on + strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4048.970 kb on - strand, within AO356_17930 at 4049.083 kb on + strand at 4049.083 kb on + strand at 4049.084 kb on - strand at 4049.084 kb on - strand at 4049.084 kb on - strand at 4049.084 kb on - strand at 4049.084 kb on - strand at 4049.084 kb on - strand at 4049.113 kb on + strand at 4049.216 kb on + strand at 4049.226 kb on - strand at 4049.340 kb on + strand, within AO356_17935 at 4049.340 kb on + strand, within AO356_17935 at 4049.341 kb on - strand, within AO356_17935 at 4049.341 kb on - strand, within AO356_17935 at 4049.341 kb on - strand, within AO356_17935 at 4049.341 kb on - strand, within AO356_17935 at 4049.341 kb on - strand, within AO356_17935 at 4049.397 kb on + strand, within AO356_17935 at 4049.397 kb on + strand, within AO356_17935 at 4049.397 kb on + strand, within AO356_17935 at 4049.472 kb on + strand, within AO356_17935 at 4049.472 kb on + strand, within AO356_17935 at 4049.473 kb on - strand, within AO356_17935 at 4049.778 kb on + strand, within AO356_17935 at 4049.778 kb on + strand, within AO356_17935 at 4049.778 kb on + strand, within AO356_17935 at 4049.778 kb on + strand, within AO356_17935 at 4049.779 kb on - strand, within AO356_17935 at 4049.779 kb on - strand, within AO356_17935 at 4049.779 kb on - strand, within AO356_17935 at 4049.779 kb on - strand, within AO356_17935 at 4049.812 kb on - strand, within AO356_17935 at 4049.812 kb on - strand, within AO356_17935 at 4049.812 kb on - strand, within AO356_17935 at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.824 kb on + strand at 4049.825 kb on - strand at 4049.825 kb on - strand at 4049.825 kb on - strand at 4049.825 kb on - strand at 4049.825 kb on - strand at 4049.825 kb on - strand at 4049.826 kb on + strand at 4049.827 kb on - strand at 4049.827 kb on - strand at 4050.275 kb on + strand, within AO356_17940 at 4050.276 kb on - strand, within AO356_17940 at 4050.276 kb on - strand, within AO356_17940 at 4050.363 kb on + strand, within AO356_17940 at 4050.363 kb on + strand, within AO356_17940 at 4050.728 kb on + strand, within AO356_17940 at 4050.728 kb on + strand, within AO356_17940 at 4050.729 kb on - strand, within AO356_17940 at 4050.729 kb on - strand, within AO356_17940 at 4050.729 kb on - strand, within AO356_17940 at 4050.729 kb on - strand, within AO356_17940 at 4050.729 kb on - strand, within AO356_17940 at 4051.035 kb on - strand, within AO356_17945 at 4051.035 kb on - strand, within AO356_17945 at 4051.035 kb on - strand, within AO356_17945 at 4051.244 kb on + strand, within AO356_17945 at 4051.244 kb on + strand, within AO356_17945 at 4051.244 kb on + strand, within AO356_17945 at 4051.245 kb on - strand, within AO356_17945 at 4051.245 kb on - strand, within AO356_17945 at 4051.484 kb on + strand at 4051.649 kb on - strand at 4051.802 kb on + strand, within AO356_17950 at 4051.803 kb on - strand, within AO356_17950
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,048,969 + AO356_17930 0.88 +0.8 4,048,969 + AO356_17930 0.88 -0.1 4,048,969 + AO356_17930 0.88 +0.5 4,048,969 + AO356_17930 0.88 -1.2 4,048,970 - AO356_17930 0.88 -1.9 4,048,970 - AO356_17930 0.88 -0.7 4,048,970 - AO356_17930 0.88 -2.5 4,048,970 - AO356_17930 0.88 +1.5 4,048,970 - AO356_17930 0.88 +0.6 4,048,970 - AO356_17930 0.88 -0.1 4,048,970 - AO356_17930 0.88 +0.2 4,048,970 - AO356_17930 0.88 +0.8 4,049,083 + +0.1 4,049,083 + -0.8 4,049,084 - +0.0 4,049,084 - +1.2 4,049,084 - -0.0 4,049,084 - +0.0 4,049,084 - +1.0 4,049,084 - -0.5 4,049,113 + -0.4 4,049,216 + +0.5 4,049,226 - +0.6 4,049,340 + AO356_17935 0.14 -0.8 4,049,340 + AO356_17935 0.14 +0.3 4,049,341 - AO356_17935 0.14 -0.1 4,049,341 - AO356_17935 0.14 +0.2 4,049,341 - AO356_17935 0.14 -0.2 4,049,341 - AO356_17935 0.14 +1.0 4,049,341 - AO356_17935 0.14 +0.2 4,049,397 + AO356_17935 0.23 +0.7 4,049,397 + AO356_17935 0.23 +0.7 4,049,397 + AO356_17935 0.23 -0.1 4,049,472 + AO356_17935 0.35 -0.4 4,049,472 + AO356_17935 0.35 -0.2 4,049,473 - AO356_17935 0.35 +0.9 4,049,778 + AO356_17935 0.83 +0.0 4,049,778 + AO356_17935 0.83 +0.6 4,049,778 + AO356_17935 0.83 -1.6 4,049,778 + AO356_17935 0.83 +0.7 4,049,779 - AO356_17935 0.83 -0.7 4,049,779 - AO356_17935 0.83 +0.0 4,049,779 - AO356_17935 0.83 +0.7 4,049,779 - AO356_17935 0.83 +0.3 4,049,812 - AO356_17935 0.88 +0.1 4,049,812 - AO356_17935 0.88 +0.5 4,049,812 - AO356_17935 0.88 +0.9 4,049,824 + +0.9 4,049,824 + -0.2 4,049,824 + -0.0 4,049,824 + +0.7 4,049,824 + +0.9 4,049,824 + +0.8 4,049,824 + +0.2 4,049,824 + +0.4 4,049,824 + -1.9 4,049,825 - +0.9 4,049,825 - +0.0 4,049,825 - -0.3 4,049,825 - -0.8 4,049,825 - -0.3 4,049,825 - +0.8 4,049,826 + +0.4 4,049,827 - +0.5 4,049,827 - +0.2 4,050,275 + AO356_17940 0.42 -0.2 4,050,276 - AO356_17940 0.42 -0.3 4,050,276 - AO356_17940 0.42 +0.2 4,050,363 + AO356_17940 0.51 -0.6 4,050,363 + AO356_17940 0.51 +0.4 4,050,728 + AO356_17940 0.90 -0.1 4,050,728 + AO356_17940 0.90 +1.0 4,050,729 - AO356_17940 0.90 -0.8 4,050,729 - AO356_17940 0.90 -0.6 4,050,729 - AO356_17940 0.90 -0.8 4,050,729 - AO356_17940 0.90 +0.3 4,050,729 - AO356_17940 0.90 +0.0 4,051,035 - AO356_17945 0.18 -0.2 4,051,035 - AO356_17945 0.18 +0.7 4,051,035 - AO356_17945 0.18 +0.4 4,051,244 + AO356_17945 0.54 +0.1 4,051,244 + AO356_17945 0.54 +0.3 4,051,244 + AO356_17945 0.54 -0.2 4,051,245 - AO356_17945 0.54 -1.0 4,051,245 - AO356_17945 0.54 +0.1 4,051,484 + +0.9 4,051,649 - -0.6 4,051,802 + AO356_17950 0.14 -2.7 4,051,803 - AO356_17950 0.14 -0.3
Or see this region's nucleotide sequence