Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3309 and Shew_3310 overlap by 4 nucleotides Shew_3310 and Shew_3311 are separated by 134 nucleotides Shew_3311 and Shew_3312 are separated by 30 nucleotides Shew_3312 and Shew_3313 are separated by 2 nucleotides Shew_3313 and Shew_3314 overlap by 4 nucleotides
Shew_3309: Shew_3309 - OstA family protein (RefSeq), at 3,950,185 to 3,950,730
_3309
Shew_3310: Shew_3310 - ABC transporter-related protein (RefSeq), at 3,950,727 to 3,951,458
_3310
Shew_3311: Shew_3311 - RNA polymerase factor sigma-54 (RefSeq), at 3,951,593 to 3,953,080
_3311
Shew_3312: Shew_3312 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 3,953,111 to 3,953,398
_3312
Shew_3313: Shew_3313 - PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq), at 3,953,401 to 3,953,844
_3313
Shew_3314: Shew_3314 - hypothetical protein (RefSeq), at 3,953,841 to 3,954,689
_3314
Position (kb)
3951
3952
3953
3954 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3951.933 kb on - strand, within Shew_3311 at 3952.179 kb on - strand, within Shew_3311 at 3952.193 kb on + strand, within Shew_3311 at 3952.738 kb on + strand, within Shew_3311 at 3953.051 kb on - strand at 3953.217 kb on - strand, within Shew_3312 at 3953.569 kb on + strand, within Shew_3313 at 3953.569 kb on + strand, within Shew_3313
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 3,951,933 - Shew_3311 0.23 -5.6 3,952,179 - Shew_3311 0.39 -6.8 3,952,193 + Shew_3311 0.40 -6.5 3,952,738 + Shew_3311 0.77 -6.3 3,953,051 - -5.2 3,953,217 - Shew_3312 0.37 +0.4 3,953,569 + Shew_3313 0.38 +0.2 3,953,569 + Shew_3313 0.38 +0.8
Or see this region's nucleotide sequence