Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3120 and Shew_3121 are separated by 152 nucleotides Shew_3121 and Shew_3122 are separated by 168 nucleotides Shew_3122 and Shew_3123 are separated by 132 nucleotides
Shew_3120: Shew_3120 - phosphate-selective porin O and P (RefSeq), at 3,707,988 to 3,709,160
_3120
Shew_3121: Shew_3121 - FMN-binding domain-containing protein (RefSeq), at 3,709,313 to 3,709,828
_3121
Shew_3122: Shew_3122 - PepSY-associated TM helix domain-containing protein (RefSeq), at 3,709,997 to 3,710,797
_3122
Shew_3123: Shew_3123 - penicillin-binding protein 1B (RefSeq), at 3,710,930 to 3,713,299
_3123
Position (kb)
3709
3710
3711 Strain fitness (log2 ratio)
-2
-1
0
1 at 3709.090 kb on - strand at 3709.240 kb on - strand at 3709.326 kb on + strand at 3709.452 kb on + strand, within Shew_3121 at 3709.644 kb on - strand, within Shew_3121 at 3709.797 kb on + strand at 3709.860 kb on - strand at 3709.896 kb on + strand at 3710.460 kb on - strand, within Shew_3122 at 3710.809 kb on + strand at 3710.809 kb on + strand at 3710.817 kb on - strand at 3710.893 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 3,709,090 - -1.6 3,709,240 - +0.9 3,709,326 + -2.6 3,709,452 + Shew_3121 0.27 -0.6 3,709,644 - Shew_3121 0.64 -0.0 3,709,797 + -0.1 3,709,860 - -1.1 3,709,896 + -2.5 3,710,460 - Shew_3122 0.58 +1.6 3,710,809 + +0.6 3,710,809 + +0.0 3,710,817 - -0.2 3,710,893 - +0.2
Or see this region's nucleotide sequence