Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2936 and Shew_2937 are separated by 11 nucleotides Shew_2937 and Shew_2938 overlap by 20 nucleotides Shew_2938 and Shew_2939 are separated by 135 nucleotides
Shew_2936: Shew_2936 - rod shape-determining protein RodA (RefSeq), at 3,475,658 to 3,476,764
_2936
Shew_2937: Shew_2937 - lytic murein transglycosylase B (RefSeq), at 3,476,776 to 3,477,783
_2937
Shew_2938: Shew_2938 - rare lipoprotein A (RefSeq), at 3,477,764 to 3,478,561
_2938
Shew_2939: Shew_2939 - Serine-type D-Ala-D-Ala carboxypeptidase (RefSeq), at 3,478,697 to 3,479,869
_2939
Position (kb) 3477
3478
3479 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 3477.809 kb on - strand at 3478.006 kb on - strand, within Shew_2938 at 3478.006 kb on - strand, within Shew_2938 at 3478.373 kb on + strand, within Shew_2938 at 3478.535 kb on + strand at 3479.131 kb on - strand, within Shew_2939 at 3479.157 kb on - strand, within Shew_2939 at 3479.231 kb on + strand, within Shew_2939 at 3479.457 kb on + strand, within Shew_2939 at 3479.518 kb on - strand, within Shew_2939
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 3,477,809 - -5.1 3,478,006 - Shew_2938 0.30 -6.7 3,478,006 - Shew_2938 0.30 -3.9 3,478,373 + Shew_2938 0.76 -2.4 3,478,535 + -1.4 3,479,131 - Shew_2939 0.37 -3.4 3,479,157 - Shew_2939 0.39 -3.4 3,479,231 + Shew_2939 0.46 -3.2 3,479,457 + Shew_2939 0.65 -3.1 3,479,518 - Shew_2939 0.70 -2.8
Or see this region's nucleotide sequence