Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2390 and pflA are separated by 103 nucleotides pflA and Shew_2392 are separated by 6 nucleotides Shew_2392 and Shew_2393 are separated by 195 nucleotides
Shew_2390: Shew_2390 - formate acetyltransferase (RefSeq), at 2,802,496 to 2,804,778
_2390
Shew_2391: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 2,804,882 to 2,805,622
pflA
Shew_2392: Shew_2392 - hypothetical protein (RefSeq), at 2,805,629 to 2,806,072
_2392
Shew_2393: Shew_2393 - acetate kinase (RefSeq), at 2,806,268 to 2,807,464
_2393
Position (kb)
2804
2805
2806 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2803.985 kb on - strand, within Shew_2390 at 2804.127 kb on + strand, within Shew_2390 at 2804.127 kb on + strand, within Shew_2390 at 2804.495 kb on - strand, within Shew_2390 at 2804.495 kb on - strand, within Shew_2390 at 2804.551 kb on - strand at 2804.663 kb on - strand at 2804.720 kb on - strand at 2805.149 kb on + strand, within pflA at 2805.154 kb on + strand, within pflA at 2805.162 kb on - strand, within pflA at 2805.162 kb on - strand, within pflA at 2805.183 kb on + strand, within pflA at 2806.022 kb on - strand, within Shew_2392 at 2806.107 kb on - strand at 2806.197 kb on + strand at 2806.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 2,803,985 - Shew_2390 0.65 -3.1 2,804,127 + Shew_2390 0.71 -1.8 2,804,127 + Shew_2390 0.71 +0.8 2,804,495 - Shew_2390 0.88 -1.5 2,804,495 - Shew_2390 0.88 -2.4 2,804,551 - -3.7 2,804,663 - -3.9 2,804,720 - +0.2 2,805,149 + pflA Shew_2391 0.36 -3.2 2,805,154 + pflA Shew_2391 0.37 -3.7 2,805,162 - pflA Shew_2391 0.38 -5.7 2,805,162 - pflA Shew_2391 0.38 -3.2 2,805,183 + pflA Shew_2391 0.41 -4.2 2,806,022 - Shew_2392 0.89 -4.7 2,806,107 - -2.2 2,806,197 + -0.7 2,806,370 - -1.2
Or see this region's nucleotide sequence