Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddl and Shew_2134 are separated by 94 nucleotides Shew_2134 and ushA are separated by 400 nucleotides ushA and Shew_2136 are separated by 604 nucleotides
Shew_2133: ddl - D-alanyl-alanine synthetase A (RefSeq), at 2,479,110 to 2,480,114
ddl
Shew_2134: Shew_2134 - heat shock protein Hsp20 (RefSeq), at 2,480,209 to 2,480,646
_2134
Shew_2135: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,481,047 to 2,482,750
ushA
Shew_2136: Shew_2136 - carboxylesterase (RefSeq), at 2,483,355 to 2,484,029
_2136
Position (kb)
2481
2482
2483 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2480.523 kb on + strand, within Shew_2134 at 2480.531 kb on - strand, within Shew_2134 at 2480.594 kb on - strand, within Shew_2134 at 2480.655 kb on - strand at 2480.760 kb on - strand at 2480.809 kb on - strand at 2480.907 kb on - strand at 2480.951 kb on - strand at 2480.974 kb on + strand at 2480.974 kb on + strand at 2481.076 kb on - strand at 2481.347 kb on - strand, within ushA at 2481.537 kb on + strand, within ushA at 2481.911 kb on - strand, within ushA at 2482.444 kb on + strand, within ushA at 2482.452 kb on - strand, within ushA at 2483.225 kb on - strand at 2483.506 kb on + strand, within Shew_2136 at 2483.514 kb on - strand, within Shew_2136 at 2483.514 kb on - strand, within Shew_2136 at 2483.708 kb on + strand, within Shew_2136 at 2483.716 kb on - strand, within Shew_2136
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 2,480,523 + Shew_2134 0.72 +0.6 2,480,531 - Shew_2134 0.74 +0.5 2,480,594 - Shew_2134 0.88 -3.1 2,480,655 - +0.3 2,480,760 - +1.2 2,480,809 - +1.0 2,480,907 - -0.5 2,480,951 - +1.5 2,480,974 + -0.8 2,480,974 + +1.1 2,481,076 - -1.1 2,481,347 - ushA Shew_2135 0.18 +1.0 2,481,537 + ushA Shew_2135 0.29 +0.8 2,481,911 - ushA Shew_2135 0.51 +0.0 2,482,444 + ushA Shew_2135 0.82 +0.7 2,482,452 - ushA Shew_2135 0.82 +1.2 2,483,225 - -0.8 2,483,506 + Shew_2136 0.22 +0.1 2,483,514 - Shew_2136 0.24 +0.0 2,483,514 - Shew_2136 0.24 +0.6 2,483,708 + Shew_2136 0.52 +0.1 2,483,716 - Shew_2136 0.53 +0.1
Or see this region's nucleotide sequence