Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0821 and Shew_0822 are separated by 66 nucleotides Shew_0822 and Shew_0823 are separated by 246 nucleotides Shew_0823 and Shew_0824 are separated by 131 nucleotides Shew_0824 and Shew_0825 are separated by 156 nucleotides
Shew_0821: Shew_0821 - mitomycin resistance protein (RefSeq), at 945,007 to 945,294
_0821
Shew_0822: Shew_0822 - lysine exporter protein LysE/YggA (RefSeq), at 945,361 to 945,954
_0822
Shew_0823: Shew_0823 - LysR family transcriptional regulator (RefSeq), at 946,201 to 947,106
_0823
Shew_0824: Shew_0824 - NAD-dependent epimerase/dehydratase (RefSeq), at 947,238 to 947,879
_0824
Shew_0825: Shew_0825 - ribose-5-phosphate isomerase A (RefSeq), at 948,036 to 948,695
_0825
Position (kb) 946
947
948 Strain fitness (log2 ratio) -1
0
1
2 at 945.535 kb on + strand, within Shew_0822 at 945.758 kb on + strand, within Shew_0822 at 945.888 kb on + strand, within Shew_0822 at 946.073 kb on + strand at 946.086 kb on + strand at 946.093 kb on - strand at 946.225 kb on - strand at 946.430 kb on + strand, within Shew_0823 at 946.678 kb on - strand, within Shew_0823 at 946.792 kb on + strand, within Shew_0823 at 946.935 kb on + strand, within Shew_0823 at 947.461 kb on + strand, within Shew_0824 at 947.588 kb on - strand, within Shew_0824 at 947.618 kb on + strand, within Shew_0824 at 947.788 kb on + strand, within Shew_0824 at 947.958 kb on + strand at 947.966 kb on - strand at 947.966 kb on - strand at 948.015 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 945,535 + Shew_0822 0.29 -1.3 945,758 + Shew_0822 0.67 -0.1 945,888 + Shew_0822 0.89 +0.3 946,073 + -1.6 946,086 + -0.5 946,093 - +0.8 946,225 - -0.3 946,430 + Shew_0823 0.25 +0.6 946,678 - Shew_0823 0.53 +0.7 946,792 + Shew_0823 0.65 -0.0 946,935 + Shew_0823 0.81 +0.7 947,461 + Shew_0824 0.35 +1.9 947,588 - Shew_0824 0.55 -0.8 947,618 + Shew_0824 0.59 -0.2 947,788 + Shew_0824 0.86 +0.3 947,958 + +1.5 947,966 - -0.5 947,966 - -0.0 948,015 + +0.8
Or see this region's nucleotide sequence