Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0582 and Shew_0583 overlap by 4 nucleotides Shew_0583 and Shew_0584 are separated by 110 nucleotides Shew_0584 and Shew_0585 are separated by 81 nucleotides
Shew_0582: Shew_0582 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 660,077 to 661,414
_0582
Shew_0583: Shew_0583 - two component transcriptional regulator (RefSeq), at 661,411 to 662,085
_0583
Shew_0584: Shew_0584 - hypothetical protein (RefSeq), at 662,196 to 662,534
_0584
Shew_0585: Shew_0585 - cAMP-regulatory protein (RefSeq), at 662,616 to 663,251
_0585
Position (kb)
662
663 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 661.216 kb on + strand, within Shew_0582 at 661.438 kb on + strand at 661.474 kb on - strand at 661.685 kb on - strand, within Shew_0583 at 661.685 kb on - strand, within Shew_0583 at 662.378 kb on + strand, within Shew_0584 at 663.121 kb on + strand, within Shew_0585 at 663.121 kb on + strand, within Shew_0585 at 663.121 kb on + strand, within Shew_0585 at 663.121 kb on + strand, within Shew_0585 at 663.121 kb on + strand, within Shew_0585 at 663.129 kb on - strand, within Shew_0585 at 663.129 kb on - strand, within Shew_0585 at 663.226 kb on + strand at 663.226 kb on + strand at 663.226 kb on + strand at 663.226 kb on + strand at 663.485 kb on + strand at 663.495 kb on + strand at 663.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 661,216 + Shew_0582 0.85 -1.5 661,438 + +0.7 661,474 - +1.5 661,685 - Shew_0583 0.41 +1.6 661,685 - Shew_0583 0.41 +0.2 662,378 + Shew_0584 0.54 +1.6 663,121 + Shew_0585 0.79 +2.8 663,121 + Shew_0585 0.79 -4.1 663,121 + Shew_0585 0.79 -0.4 663,121 + Shew_0585 0.79 -1.7 663,121 + Shew_0585 0.79 +2.6 663,129 - Shew_0585 0.81 -0.7 663,129 - Shew_0585 0.81 +2.7 663,226 + -1.0 663,226 + -2.4 663,226 + +1.3 663,226 + -2.7 663,485 + -0.8 663,495 + -2.4 663,522 - -0.4
Or see this region's nucleotide sequence