Experiment: MoYLS4 with Nitrite 0.025mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and ilvN-1 are separated by 432 nucleotides ilvN-1 and ptB are separated by 3 nucleotides
DVU0625: nrfA - cytochrome c nitrite reductase, catalytic subunit NrfA, putative (Shelley Haveman), at 691,544 to 693,118
nrfA
DVU0626: ilvN-1 - acetolactate synthase, small subunit (TIGR), at 693,551 to 694,030
ilvN-1
DVU0627: ptB - phosphotransbutyrylase (TIGR), at 694,034 to 695,065
ptB
Position (kb) 693
694
695 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 692.658 kb on + strand, within nrfA at 692.658 kb on + strand, within nrfA at 692.708 kb on + strand, within nrfA at 692.770 kb on - strand, within nrfA at 692.776 kb on - strand, within nrfA at 692.959 kb on + strand, within nrfA at 693.037 kb on - strand at 693.061 kb on + strand at 693.061 kb on + strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.076 kb on + strand at 693.093 kb on + strand at 693.095 kb on + strand at 693.103 kb on - strand at 693.140 kb on + strand at 693.148 kb on - strand at 693.185 kb on + strand at 693.295 kb on + strand at 693.301 kb on - strand at 693.312 kb on + strand at 693.330 kb on - strand at 693.332 kb on + strand at 693.353 kb on + strand at 693.479 kb on + strand at 693.506 kb on - strand at 693.513 kb on + strand at 693.521 kb on - strand at 693.530 kb on + strand at 693.616 kb on - strand, within ilvN-1 at 693.628 kb on - strand, within ilvN-1 at 693.735 kb on - strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.904 kb on + strand, within ilvN-1 at 693.947 kb on + strand, within ilvN-1 at 693.984 kb on + strand at 693.984 kb on + strand at 694.051 kb on - strand at 694.085 kb on - strand at 694.113 kb on + strand at 694.231 kb on - strand, within ptB at 694.294 kb on - strand, within ptB at 694.361 kb on + strand, within ptB at 694.414 kb on - strand, within ptB at 694.423 kb on + strand, within ptB at 694.454 kb on - strand, within ptB at 694.537 kb on + strand, within ptB at 694.593 kb on - strand, within ptB at 694.609 kb on + strand, within ptB at 694.647 kb on + strand, within ptB at 694.738 kb on - strand, within ptB at 694.797 kb on + strand, within ptB at 694.805 kb on - strand, within ptB at 694.839 kb on - strand, within ptB at 694.897 kb on + strand, within ptB
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nitrite 0.025mg/mL remove 692,658 + nrfA DVU0625 0.71 -2.5 692,658 + nrfA DVU0625 0.71 -1.5 692,708 + nrfA DVU0625 0.74 -3.4 692,770 - nrfA DVU0625 0.78 +1.2 692,776 - nrfA DVU0625 0.78 -3.3 692,959 + nrfA DVU0625 0.90 +1.2 693,037 - -2.7 693,061 + -3.2 693,061 + -2.1 693,069 - -2.8 693,069 - -3.1 693,069 - -3.0 693,069 - -2.9 693,069 - -2.8 693,069 - -2.6 693,069 - +0.6 693,076 + -2.4 693,093 + -2.2 693,095 + -2.9 693,103 - -3.1 693,140 + -2.4 693,148 - -2.5 693,185 + +0.0 693,295 + -0.0 693,301 - -0.2 693,312 + +0.2 693,330 - +0.1 693,332 + -0.5 693,353 + -0.2 693,479 + +0.0 693,506 - -0.1 693,513 + +0.1 693,521 - +0.0 693,530 + +1.2 693,616 - ilvN-1 DVU0626 0.14 +0.3 693,628 - ilvN-1 DVU0626 0.16 -0.5 693,735 - ilvN-1 DVU0626 0.38 -0.2 693,814 + ilvN-1 DVU0626 0.55 -0.5 693,814 + ilvN-1 DVU0626 0.55 -0.2 693,814 + ilvN-1 DVU0626 0.55 -0.0 693,822 - ilvN-1 DVU0626 0.56 -0.1 693,822 - ilvN-1 DVU0626 0.56 -0.4 693,822 - ilvN-1 DVU0626 0.56 +1.4 693,822 - ilvN-1 DVU0626 0.56 -0.5 693,822 - ilvN-1 DVU0626 0.56 -0.6 693,822 - ilvN-1 DVU0626 0.56 -0.2 693,822 - ilvN-1 DVU0626 0.56 +0.8 693,904 + ilvN-1 DVU0626 0.74 +0.0 693,947 + ilvN-1 DVU0626 0.82 -0.1 693,984 + +0.1 693,984 + +0.1 694,051 - +0.1 694,085 - -0.0 694,113 + -1.3 694,231 - ptB DVU0627 0.19 +0.1 694,294 - ptB DVU0627 0.25 -0.1 694,361 + ptB DVU0627 0.32 +0.4 694,414 - ptB DVU0627 0.37 +0.0 694,423 + ptB DVU0627 0.38 +0.3 694,454 - ptB DVU0627 0.41 -0.4 694,537 + ptB DVU0627 0.49 +0.2 694,593 - ptB DVU0627 0.54 -0.4 694,609 + ptB DVU0627 0.56 -0.2 694,647 + ptB DVU0627 0.59 +1.2 694,738 - ptB DVU0627 0.68 -0.4 694,797 + ptB DVU0627 0.74 -0.6 694,805 - ptB DVU0627 0.75 -0.1 694,839 - ptB DVU0627 0.78 -0.5 694,897 + ptB DVU0627 0.84 +0.0
Or see this region's nucleotide sequence