Experiment: MoYLS4 with Nitrite 0.025mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potD-1 and potC are separated by 1 nucleotides potC and potB are separated by 2 nucleotides potB and potA overlap by 14 nucleotides
DVU0095: potD-1 - polyamine ABC transporter, periplasmic polyamine-binding protein (TIGR), at 125,677 to 126,714
potD-1
DVU0096: potC - polyamine ABC transporter, permease protein (TIGR), at 126,716 to 127,489
potC
DVU0097: potB - polyamine ABC transporter, permease protein (TIGR), at 127,492 to 128,379
potB
DVU0098: potA - polyamine ABC transporter, ATP-binding protein (TIGR), at 128,366 to 129,472
potA
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 126.553 kb on + strand, within potD-1 at 126.561 kb on - strand, within potD-1 at 126.592 kb on + strand, within potD-1 at 126.592 kb on + strand, within potD-1 at 126.600 kb on - strand, within potD-1 at 126.633 kb on - strand at 126.671 kb on + strand at 126.679 kb on - strand at 126.679 kb on - strand at 126.731 kb on - strand at 126.734 kb on + strand at 126.754 kb on + strand at 126.760 kb on + strand at 126.884 kb on + strand, within potC at 126.889 kb on - strand, within potC at 126.954 kb on - strand, within potC at 126.999 kb on - strand, within potC at 127.008 kb on + strand, within potC at 127.027 kb on - strand, within potC at 127.149 kb on + strand, within potC at 127.171 kb on + strand, within potC at 127.187 kb on - strand, within potC at 127.194 kb on + strand, within potC at 127.199 kb on + strand, within potC at 127.199 kb on + strand, within potC at 127.199 kb on + strand, within potC at 127.207 kb on - strand, within potC at 127.207 kb on - strand, within potC at 127.207 kb on - strand, within potC at 127.207 kb on - strand, within potC at 127.304 kb on + strand, within potC at 127.312 kb on - strand, within potC at 127.387 kb on + strand, within potC at 127.395 kb on - strand, within potC at 127.417 kb on + strand at 127.425 kb on - strand at 127.426 kb on - strand at 127.450 kb on - strand at 127.451 kb on - strand at 127.453 kb on + strand at 127.461 kb on - strand at 127.520 kb on - strand at 127.539 kb on - strand at 127.574 kb on + strand at 127.582 kb on - strand, within potB at 127.830 kb on - strand, within potB at 127.850 kb on - strand, within potB at 127.889 kb on + strand, within potB at 127.913 kb on + strand, within potB at 127.993 kb on - strand, within potB at 128.120 kb on + strand, within potB at 128.125 kb on + strand, within potB at 128.125 kb on + strand, within potB at 128.222 kb on + strand, within potB at 128.262 kb on - strand, within potB at 128.300 kb on + strand at 128.363 kb on + strand at 128.516 kb on + strand, within potA at 128.529 kb on + strand, within potA at 128.531 kb on + strand, within potA at 128.537 kb on - strand, within potA at 128.537 kb on - strand, within potA at 128.539 kb on - strand, within potA at 128.539 kb on - strand, within potA at 128.587 kb on + strand, within potA at 128.608 kb on + strand, within potA at 128.616 kb on - strand, within potA at 128.636 kb on - strand, within potA at 128.643 kb on - strand, within potA at 128.679 kb on - strand, within potA at 128.687 kb on + strand, within potA at 128.710 kb on - strand, within potA at 128.772 kb on + strand, within potA at 128.772 kb on + strand, within potA at 128.819 kb on - strand, within potA at 128.905 kb on - strand, within potA at 128.905 kb on - strand, within potA at 128.942 kb on - strand, within potA at 128.969 kb on - strand, within potA at 128.981 kb on - strand, within potA at 128.983 kb on - strand, within potA at 129.011 kb on + strand, within potA at 129.051 kb on - strand, within potA at 129.251 kb on + strand, within potA at 129.322 kb on + strand, within potA at 129.351 kb on + strand, within potA
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nitrite 0.025mg/mL remove 126,553 + potD-1 DVU0095 0.84 -0.2 126,561 - potD-1 DVU0095 0.85 +0.2 126,592 + potD-1 DVU0095 0.88 +0.6 126,592 + potD-1 DVU0095 0.88 +0.2 126,600 - potD-1 DVU0095 0.89 -0.1 126,633 - -0.1 126,671 + -0.2 126,679 - +0.0 126,679 - +0.0 126,731 - +0.0 126,734 + -0.1 126,754 + -0.2 126,760 + -0.0 126,884 + potC DVU0096 0.22 +0.2 126,889 - potC DVU0096 0.22 -0.8 126,954 - potC DVU0096 0.31 -0.2 126,999 - potC DVU0096 0.37 +0.3 127,008 + potC DVU0096 0.38 +0.1 127,027 - potC DVU0096 0.40 -0.1 127,149 + potC DVU0096 0.56 +0.2 127,171 + potC DVU0096 0.59 -1.1 127,187 - potC DVU0096 0.61 -0.0 127,194 + potC DVU0096 0.62 -0.4 127,199 + potC DVU0096 0.62 -1.1 127,199 + potC DVU0096 0.62 -0.0 127,199 + potC DVU0096 0.62 +0.6 127,207 - potC DVU0096 0.63 +0.0 127,207 - potC DVU0096 0.63 -1.0 127,207 - potC DVU0096 0.63 +0.4 127,207 - potC DVU0096 0.63 +0.3 127,304 + potC DVU0096 0.76 -0.5 127,312 - potC DVU0096 0.77 -0.2 127,387 + potC DVU0096 0.87 -0.1 127,395 - potC DVU0096 0.88 +0.2 127,417 + -0.2 127,425 - +0.4 127,426 - +0.2 127,450 - -0.1 127,451 - -0.7 127,453 + +0.0 127,461 - -0.0 127,520 - +0.2 127,539 - +0.2 127,574 + +0.4 127,582 - potB DVU0097 0.10 -0.6 127,830 - potB DVU0097 0.38 -0.9 127,850 - potB DVU0097 0.40 -0.3 127,889 + potB DVU0097 0.45 +0.9 127,913 + potB DVU0097 0.47 +0.2 127,993 - potB DVU0097 0.56 -0.4 128,120 + potB DVU0097 0.71 +2.2 128,125 + potB DVU0097 0.71 -2.6 128,125 + potB DVU0097 0.71 -1.4 128,222 + potB DVU0097 0.82 -0.5 128,262 - potB DVU0097 0.87 -0.0 128,300 + -0.0 128,363 + -0.7 128,516 + potA DVU0098 0.14 -0.1 128,529 + potA DVU0098 0.15 -0.1 128,531 + potA DVU0098 0.15 +1.8 128,537 - potA DVU0098 0.15 -0.3 128,537 - potA DVU0098 0.15 +1.2 128,539 - potA DVU0098 0.16 -0.5 128,539 - potA DVU0098 0.16 -0.1 128,587 + potA DVU0098 0.20 -0.1 128,608 + potA DVU0098 0.22 -0.1 128,616 - potA DVU0098 0.23 -0.3 128,636 - potA DVU0098 0.24 +0.2 128,643 - potA DVU0098 0.25 -0.0 128,679 - potA DVU0098 0.28 -0.0 128,687 + potA DVU0098 0.29 -0.1 128,710 - potA DVU0098 0.31 +0.8 128,772 + potA DVU0098 0.37 -0.0 128,772 + potA DVU0098 0.37 -0.3 128,819 - potA DVU0098 0.41 +1.0 128,905 - potA DVU0098 0.49 +2.8 128,905 - potA DVU0098 0.49 -0.1 128,942 - potA DVU0098 0.52 +0.3 128,969 - potA DVU0098 0.54 -0.1 128,981 - potA DVU0098 0.56 -0.4 128,983 - potA DVU0098 0.56 -0.4 129,011 + potA DVU0098 0.58 -0.2 129,051 - potA DVU0098 0.62 -0.5 129,251 + potA DVU0098 0.80 +1.2 129,322 + potA DVU0098 0.86 +0.1 129,351 + potA DVU0098 0.89 -0.2
Or see this region's nucleotide sequence