Experiment: MoYLS4 with Nitrite 0.025mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proA and nadD are separated by 25 nucleotides nadD and DVU1955 overlap by 4 nucleotides DVU1955 and DVU1956 are separated by 141 nucleotides
DVU1953: proA - gamma-glutamyl phosphate reductase (TIGR), at 2,026,227 to 2,027,486
proA
DVU1954: nadD - nicotinate (nicotinamide) nucleotide adenylyltransferase (TIGR), at 2,027,512 to 2,028,216
nadD
DVU1955: DVU1955 - TPR domain protein (TIGR), at 2,028,213 to 2,029,046
DVU1955
DVU1956: DVU1956 - heptosyltransferase family protein (TIGR), at 2,029,188 to 2,030,243
DVU1956
Position (kb)
2028
2029
2030 Strain fitness (log2 ratio)
-2
-1
0
1 at 2027.237 kb on + strand, within proA at 2027.385 kb on + strand at 2028.181 kb on + strand at 2028.387 kb on - strand, within DVU1955 at 2028.447 kb on + strand, within DVU1955 at 2028.561 kb on - strand, within DVU1955 at 2028.644 kb on - strand, within DVU1955 at 2028.686 kb on + strand, within DVU1955 at 2028.716 kb on + strand, within DVU1955 at 2028.833 kb on - strand, within DVU1955 at 2028.855 kb on + strand, within DVU1955 at 2028.860 kb on + strand, within DVU1955 at 2028.863 kb on - strand, within DVU1955 at 2028.863 kb on - strand, within DVU1955 at 2028.863 kb on - strand, within DVU1955 at 2028.890 kb on + strand, within DVU1955 at 2028.898 kb on - strand, within DVU1955 at 2028.898 kb on - strand, within DVU1955 at 2028.898 kb on - strand, within DVU1955 at 2028.902 kb on + strand, within DVU1955 at 2028.922 kb on + strand, within DVU1955 at 2029.173 kb on + strand at 2029.181 kb on - strand at 2029.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nitrite 0.025mg/mL remove 2,027,237 + proA DVU1953 0.80 -0.9 2,027,385 + +1.2 2,028,181 + -0.3 2,028,387 - DVU1955 0.21 -0.2 2,028,447 + DVU1955 0.28 -0.8 2,028,561 - DVU1955 0.42 -0.4 2,028,644 - DVU1955 0.52 -0.4 2,028,686 + DVU1955 0.57 -0.4 2,028,716 + DVU1955 0.60 -0.6 2,028,833 - DVU1955 0.74 -0.4 2,028,855 + DVU1955 0.77 -2.2 2,028,860 + DVU1955 0.78 -0.1 2,028,863 - DVU1955 0.78 -0.5 2,028,863 - DVU1955 0.78 -0.2 2,028,863 - DVU1955 0.78 -1.1 2,028,890 + DVU1955 0.81 -0.9 2,028,898 - DVU1955 0.82 +0.0 2,028,898 - DVU1955 0.82 +0.1 2,028,898 - DVU1955 0.82 -1.2 2,028,902 + DVU1955 0.83 +0.0 2,028,922 + DVU1955 0.85 -0.8 2,029,173 + -2.0 2,029,181 - +0.3 2,029,260 - +1.6
Or see this region's nucleotide sequence