Experiment: MoYLS4 with Nitrite 0.025mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pilT-1 and pppA are separated by 175 nucleotides pppA and DVU1264 are separated by 307 nucleotides
DVU1262: pilT-1 - twitching motility protein PilT (TIGR), at 1,350,023 to 1,351,132
pilT-1
DVU1263: pppA - type IV prepilin-like proteins leader peptidase (TIGR), at 1,351,308 to 1,352,111
pppA
DVU1264: DVU1264 - transglycosylase SLT domain protein (TIGR), at 1,352,419 to 1,353,180
DVU1264
Position (kb)
1351
1352
1353 Strain fitness (log2 ratio)
-1
0
1 at 1350.331 kb on + strand, within pilT-1 at 1350.534 kb on + strand, within pilT-1 at 1350.620 kb on + strand, within pilT-1 at 1350.759 kb on - strand, within pilT-1 at 1350.920 kb on + strand, within pilT-1 at 1350.922 kb on - strand, within pilT-1 at 1350.972 kb on - strand, within pilT-1 at 1350.979 kb on + strand, within pilT-1 at 1351.014 kb on + strand, within pilT-1 at 1351.058 kb on + strand at 1351.065 kb on + strand at 1351.073 kb on + strand at 1351.139 kb on + strand at 1351.147 kb on - strand at 1351.183 kb on - strand at 1351.209 kb on + strand at 1351.269 kb on - strand at 1351.329 kb on - strand at 1351.527 kb on + strand, within pppA at 1351.541 kb on + strand, within pppA at 1351.615 kb on - strand, within pppA at 1351.632 kb on + strand, within pppA at 1351.790 kb on - strand, within pppA at 1351.790 kb on - strand, within pppA at 1351.839 kb on + strand, within pppA at 1351.851 kb on + strand, within pppA at 1351.861 kb on - strand, within pppA at 1351.877 kb on + strand, within pppA at 1352.138 kb on + strand at 1352.253 kb on - strand at 1352.282 kb on - strand at 1352.339 kb on + strand at 1352.342 kb on - strand at 1352.347 kb on - strand at 1352.402 kb on - strand at 1352.404 kb on - strand at 1352.406 kb on - strand at 1352.415 kb on + strand at 1352.496 kb on - strand, within DVU1264 at 1352.577 kb on + strand, within DVU1264 at 1352.605 kb on - strand, within DVU1264 at 1352.617 kb on + strand, within DVU1264 at 1352.642 kb on - strand, within DVU1264 at 1352.702 kb on - strand, within DVU1264 at 1352.745 kb on + strand, within DVU1264 at 1352.796 kb on - strand, within DVU1264 at 1353.004 kb on - strand, within DVU1264 at 1353.065 kb on + strand, within DVU1264 at 1353.075 kb on + strand, within DVU1264 at 1353.078 kb on - strand, within DVU1264 at 1353.083 kb on - strand, within DVU1264 at 1353.083 kb on - strand, within DVU1264 at 1353.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nitrite 0.025mg/mL remove 1,350,331 + pilT-1 DVU1262 0.28 +0.2 1,350,534 + pilT-1 DVU1262 0.46 -0.5 1,350,620 + pilT-1 DVU1262 0.54 -0.5 1,350,759 - pilT-1 DVU1262 0.66 -0.7 1,350,920 + pilT-1 DVU1262 0.81 -0.1 1,350,922 - pilT-1 DVU1262 0.81 +0.4 1,350,972 - pilT-1 DVU1262 0.85 -0.0 1,350,979 + pilT-1 DVU1262 0.86 -0.2 1,351,014 + pilT-1 DVU1262 0.89 +0.5 1,351,058 + -0.4 1,351,065 + -0.3 1,351,073 + -0.4 1,351,139 + +0.1 1,351,147 - +0.2 1,351,183 - +0.1 1,351,209 + +0.0 1,351,269 - +0.2 1,351,329 - -0.2 1,351,527 + pppA DVU1263 0.27 -0.6 1,351,541 + pppA DVU1263 0.29 -0.4 1,351,615 - pppA DVU1263 0.38 -0.3 1,351,632 + pppA DVU1263 0.40 -0.6 1,351,790 - pppA DVU1263 0.60 -0.2 1,351,790 - pppA DVU1263 0.60 -0.5 1,351,839 + pppA DVU1263 0.66 -0.7 1,351,851 + pppA DVU1263 0.68 -0.6 1,351,861 - pppA DVU1263 0.69 -0.8 1,351,877 + pppA DVU1263 0.71 +1.5 1,352,138 + -0.6 1,352,253 - -0.3 1,352,282 - -0.5 1,352,339 + +0.3 1,352,342 - -0.2 1,352,347 - -0.1 1,352,402 - +0.8 1,352,404 - -0.6 1,352,406 - +0.1 1,352,415 + +0.1 1,352,496 - DVU1264 0.10 +0.6 1,352,577 + DVU1264 0.21 -0.3 1,352,605 - DVU1264 0.24 -0.6 1,352,617 + DVU1264 0.26 -1.1 1,352,642 - DVU1264 0.29 -1.4 1,352,702 - DVU1264 0.37 -0.3 1,352,745 + DVU1264 0.43 -1.2 1,352,796 - DVU1264 0.49 -0.6 1,353,004 - DVU1264 0.77 -0.8 1,353,065 + DVU1264 0.85 +0.2 1,353,075 + DVU1264 0.86 +0.2 1,353,078 - DVU1264 0.86 +0.3 1,353,083 - DVU1264 0.87 -0.3 1,353,083 - DVU1264 0.87 -0.6 1,353,109 + -0.5
Or see this region's nucleotide sequence