Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2264 and Pf6N2E2_2265 are separated by 61 nucleotides Pf6N2E2_2265 and Pf6N2E2_2266 are separated by 157 nucleotides Pf6N2E2_2266 and Pf6N2E2_2267 overlap by 4 nucleotides
Pf6N2E2_2264 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 2,678,806 to 2,679,636
_2264
Pf6N2E2_2265 - Copper tolerance protein, at 2,679,698 to 2,680,243
_2265
Pf6N2E2_2266 - Copper-sensing two-component system response regulator CusR, at 2,680,401 to 2,681,081
_2266
Pf6N2E2_2267 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 2,681,078 to 2,682,436
_2267
Position (kb)
2680
2681
2682 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2679.590 kb on + strand at 2679.591 kb on - strand at 2679.669 kb on + strand at 2679.670 kb on - strand at 2679.699 kb on + strand at 2679.699 kb on + strand at 2679.699 kb on + strand at 2679.700 kb on - strand at 2679.738 kb on + strand at 2679.776 kb on - strand, within Pf6N2E2_2265 at 2679.836 kb on - strand, within Pf6N2E2_2265 at 2680.030 kb on + strand, within Pf6N2E2_2265 at 2680.031 kb on - strand, within Pf6N2E2_2265 at 2680.031 kb on - strand, within Pf6N2E2_2265 at 2680.031 kb on - strand, within Pf6N2E2_2265 at 2680.041 kb on - strand, within Pf6N2E2_2265 at 2680.193 kb on - strand at 2680.193 kb on - strand at 2680.193 kb on - strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.249 kb on + strand at 2680.250 kb on - strand at 2680.250 kb on - strand at 2680.250 kb on - strand at 2680.262 kb on + strand at 2680.262 kb on + strand at 2680.262 kb on + strand at 2680.262 kb on + strand at 2680.263 kb on - strand at 2680.263 kb on - strand at 2680.263 kb on - strand at 2680.263 kb on - strand at 2680.286 kb on + strand at 2680.286 kb on + strand at 2680.286 kb on + strand at 2680.287 kb on - strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.324 kb on - strand at 2680.324 kb on - strand at 2680.324 kb on - strand at 2680.364 kb on + strand at 2680.364 kb on + strand at 2680.365 kb on - strand at 2680.365 kb on - strand at 2680.365 kb on - strand at 2680.365 kb on - strand at 2680.378 kb on + strand at 2680.378 kb on + strand at 2680.443 kb on + strand at 2680.444 kb on - strand at 2680.533 kb on + strand, within Pf6N2E2_2266 at 2680.616 kb on + strand, within Pf6N2E2_2266 at 2680.617 kb on - strand, within Pf6N2E2_2266 at 2680.692 kb on + strand, within Pf6N2E2_2266 at 2680.693 kb on - strand, within Pf6N2E2_2266 at 2680.693 kb on - strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.056 kb on - strand at 2681.056 kb on - strand at 2681.056 kb on - strand at 2681.352 kb on - strand, within Pf6N2E2_2267 at 2681.799 kb on + strand, within Pf6N2E2_2267 at 2681.800 kb on - strand, within Pf6N2E2_2267 at 2681.800 kb on - strand, within Pf6N2E2_2267 at 2681.800 kb on - strand, within Pf6N2E2_2267 at 2681.939 kb on + strand, within Pf6N2E2_2267 at 2681.939 kb on + strand, within Pf6N2E2_2267
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,679,590 + -0.0 2,679,591 - -0.1 2,679,669 + -0.8 2,679,670 - -0.9 2,679,699 + +1.1 2,679,699 + +0.2 2,679,699 + +1.8 2,679,700 - -0.3 2,679,738 + +0.2 2,679,776 - Pf6N2E2_2265 0.14 -0.0 2,679,836 - Pf6N2E2_2265 0.25 +1.2 2,680,030 + Pf6N2E2_2265 0.61 +0.7 2,680,031 - Pf6N2E2_2265 0.61 -0.4 2,680,031 - Pf6N2E2_2265 0.61 +0.2 2,680,031 - Pf6N2E2_2265 0.61 +0.6 2,680,041 - Pf6N2E2_2265 0.63 +1.0 2,680,193 - -0.1 2,680,193 - +0.7 2,680,193 - -1.4 2,680,243 + +0.4 2,680,243 + +0.4 2,680,243 + +1.2 2,680,243 + +0.6 2,680,243 + +1.8 2,680,243 + +0.6 2,680,243 + +0.1 2,680,243 + +0.9 2,680,244 - +2.3 2,680,244 - -0.3 2,680,244 - +0.9 2,680,244 - +0.8 2,680,244 - -0.2 2,680,244 - -0.2 2,680,244 - -0.9 2,680,244 - -0.6 2,680,244 - +0.3 2,680,244 - +1.8 2,680,249 + +0.4 2,680,250 - +0.2 2,680,250 - +1.5 2,680,250 - +0.4 2,680,262 + +1.2 2,680,262 + -1.2 2,680,262 + -0.5 2,680,262 + +0.8 2,680,263 - +0.6 2,680,263 - -0.1 2,680,263 - -0.8 2,680,263 - -1.2 2,680,286 + +0.6 2,680,286 + +0.8 2,680,286 + +0.8 2,680,287 - +0.3 2,680,323 + +0.9 2,680,323 + -0.8 2,680,323 + +0.3 2,680,323 + -0.3 2,680,323 + +0.4 2,680,323 + -0.0 2,680,324 - -0.4 2,680,324 - -0.5 2,680,324 - -0.2 2,680,364 + -0.3 2,680,364 + +1.1 2,680,365 - +0.4 2,680,365 - -0.0 2,680,365 - -0.1 2,680,365 - +0.5 2,680,378 + -0.5 2,680,378 + -0.7 2,680,443 + +0.8 2,680,444 - +0.2 2,680,533 + Pf6N2E2_2266 0.19 -0.5 2,680,616 + Pf6N2E2_2266 0.32 -3.1 2,680,617 - Pf6N2E2_2266 0.32 +0.3 2,680,692 + Pf6N2E2_2266 0.43 -1.9 2,680,693 - Pf6N2E2_2266 0.43 -0.1 2,680,693 - -0.2 2,681,055 + +0.6 2,681,055 + +0.4 2,681,055 + -0.5 2,681,055 + -1.1 2,681,055 + +0.5 2,681,055 + -0.7 2,681,056 - +0.9 2,681,056 - -0.4 2,681,056 - -1.2 2,681,352 - Pf6N2E2_2267 0.20 -0.6 2,681,799 + Pf6N2E2_2267 0.53 -0.5 2,681,800 - Pf6N2E2_2267 0.53 +1.6 2,681,800 - Pf6N2E2_2267 0.53 -0.7 2,681,800 - Pf6N2E2_2267 0.53 -0.8 2,681,939 + Pf6N2E2_2267 0.63 +0.9 2,681,939 + Pf6N2E2_2267 0.63 -0.8
Or see this region's nucleotide sequence