Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_177 and Pf6N2E2_178 are separated by 35 nucleotides Pf6N2E2_178 and Pf6N2E2_179 are separated by 3 nucleotides Pf6N2E2_179 and Pf6N2E2_180 overlap by 20 nucleotides Pf6N2E2_180 and Pf6N2E2_6104 are separated by 90 nucleotides
Pf6N2E2_177 - Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20), at 226,871 to 227,887
_177
Pf6N2E2_178 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 227,923 to 230,301
_178
Pf6N2E2_179 - Integration host factor alpha subunit, at 230,305 to 230,607
_179
Pf6N2E2_180 - Transcriptional regulator, MerR family, at 230,588 to 230,944
_180
Pf6N2E2_6104 - tRNA-Pro-GGG, at 231,035 to 231,108
_6104
Position (kb) 227
228
229
230
231 Strain fitness (log2 ratio) -2
-1
0
1 at 229.328 kb on - strand, within Pf6N2E2_178 at 230.304 kb on + strand at 230.304 kb on + strand at 230.304 kb on + strand at 230.305 kb on - strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.588 kb on - strand at 230.588 kb on - strand at 230.605 kb on + strand at 230.756 kb on + strand, within Pf6N2E2_180 at 230.756 kb on + strand, within Pf6N2E2_180 at 230.756 kb on + strand, within Pf6N2E2_180 at 230.804 kb on + strand, within Pf6N2E2_180 at 230.869 kb on + strand, within Pf6N2E2_180 at 230.870 kb on - strand, within Pf6N2E2_180 at 231.128 kb on - strand at 231.218 kb on - strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 229,328 - Pf6N2E2_178 0.59 -2.1 230,304 + -0.5 230,304 + +0.3 230,304 + -1.7 230,305 - -0.6 230,587 + +0.3 230,587 + -1.0 230,587 + -0.0 230,587 + -0.5 230,587 + +0.4 230,588 - +0.2 230,588 - -0.3 230,605 + -0.1 230,756 + Pf6N2E2_180 0.47 +0.0 230,756 + Pf6N2E2_180 0.47 -1.1 230,756 + Pf6N2E2_180 0.47 -2.7 230,804 + Pf6N2E2_180 0.61 +1.3 230,869 + Pf6N2E2_180 0.79 -0.3 230,870 - Pf6N2E2_180 0.79 +1.4 231,128 - +0.2 231,218 - +1.1 231,283 + +0.4 231,283 + +1.1 231,283 + -0.9 231,283 + +1.7 231,283 + -1.6 231,283 + +0.7 231,284 - +0.5
Or see this region's nucleotide sequence