Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1701 and Pf6N2E2_1702 are separated by 65 nucleotides Pf6N2E2_1702 and Pf6N2E2_1703 are separated by 105 nucleotides
Pf6N2E2_1701 - ABC transporter ATP-binding protein, at 2,009,558 to 2,010,334
_1701
Pf6N2E2_1702 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 2,010,400 to 2,011,734
_1702
Pf6N2E2_1703 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,011,840 to 2,012,904
_1703
Position (kb)
2010
2011
2012 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2009.504 kb on - strand at 2009.658 kb on + strand, within Pf6N2E2_1701 at 2009.664 kb on + strand, within Pf6N2E2_1701 at 2009.665 kb on - strand, within Pf6N2E2_1701 at 2009.922 kb on + strand, within Pf6N2E2_1701 at 2009.926 kb on + strand, within Pf6N2E2_1701 at 2009.926 kb on + strand, within Pf6N2E2_1701 at 2010.401 kb on + strand at 2010.761 kb on + strand, within Pf6N2E2_1702 at 2010.761 kb on + strand, within Pf6N2E2_1702 at 2010.762 kb on - strand, within Pf6N2E2_1702 at 2010.762 kb on - strand, within Pf6N2E2_1702 at 2010.821 kb on + strand, within Pf6N2E2_1702 at 2010.822 kb on - strand, within Pf6N2E2_1702 at 2010.884 kb on + strand, within Pf6N2E2_1702 at 2010.884 kb on + strand, within Pf6N2E2_1702 at 2011.017 kb on + strand, within Pf6N2E2_1702 at 2011.018 kb on - strand, within Pf6N2E2_1702 at 2011.121 kb on + strand, within Pf6N2E2_1702 at 2011.122 kb on - strand, within Pf6N2E2_1702 at 2011.122 kb on - strand, within Pf6N2E2_1702 at 2011.241 kb on + strand, within Pf6N2E2_1702 at 2011.242 kb on - strand, within Pf6N2E2_1702 at 2011.242 kb on - strand, within Pf6N2E2_1702 at 2011.269 kb on - strand, within Pf6N2E2_1702 at 2011.269 kb on - strand, within Pf6N2E2_1702 at 2011.389 kb on - strand, within Pf6N2E2_1702 at 2011.416 kb on - strand, within Pf6N2E2_1702 at 2011.460 kb on + strand, within Pf6N2E2_1702 at 2011.460 kb on + strand, within Pf6N2E2_1702 at 2011.553 kb on + strand, within Pf6N2E2_1702 at 2011.563 kb on - strand, within Pf6N2E2_1702 at 2011.604 kb on + strand at 2011.714 kb on - strand at 2011.714 kb on - strand at 2011.714 kb on - strand at 2012.364 kb on - strand, within Pf6N2E2_1703 at 2012.438 kb on + strand, within Pf6N2E2_1703 at 2012.438 kb on + strand, within Pf6N2E2_1703 at 2012.438 kb on + strand, within Pf6N2E2_1703 at 2012.439 kb on - strand, within Pf6N2E2_1703 at 2012.439 kb on - strand, within Pf6N2E2_1703 at 2012.439 kb on - strand, within Pf6N2E2_1703 at 2012.444 kb on + strand, within Pf6N2E2_1703 at 2012.445 kb on - strand at 2012.513 kb on + strand, within Pf6N2E2_1703 at 2012.513 kb on + strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.634 kb on - strand, within Pf6N2E2_1703 at 2012.693 kb on + strand, within Pf6N2E2_1703
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,009,504 - -1.4 2,009,658 + Pf6N2E2_1701 0.13 +1.5 2,009,664 + Pf6N2E2_1701 0.14 +0.3 2,009,665 - Pf6N2E2_1701 0.14 +3.2 2,009,922 + Pf6N2E2_1701 0.47 -1.2 2,009,926 + Pf6N2E2_1701 0.47 +1.4 2,009,926 + Pf6N2E2_1701 0.47 +0.9 2,010,401 + +0.1 2,010,761 + Pf6N2E2_1702 0.27 +2.8 2,010,761 + Pf6N2E2_1702 0.27 -0.3 2,010,762 - Pf6N2E2_1702 0.27 -1.9 2,010,762 - Pf6N2E2_1702 0.27 +0.7 2,010,821 + Pf6N2E2_1702 0.32 -0.4 2,010,822 - Pf6N2E2_1702 0.32 +0.4 2,010,884 + Pf6N2E2_1702 0.36 -0.6 2,010,884 + Pf6N2E2_1702 0.36 -1.4 2,011,017 + Pf6N2E2_1702 0.46 -0.3 2,011,018 - Pf6N2E2_1702 0.46 +0.4 2,011,121 + Pf6N2E2_1702 0.54 -2.4 2,011,122 - Pf6N2E2_1702 0.54 -0.7 2,011,122 - Pf6N2E2_1702 0.54 +0.1 2,011,241 + Pf6N2E2_1702 0.63 +0.4 2,011,242 - Pf6N2E2_1702 0.63 +0.1 2,011,242 - Pf6N2E2_1702 0.63 -0.7 2,011,269 - Pf6N2E2_1702 0.65 +1.6 2,011,269 - Pf6N2E2_1702 0.65 +0.4 2,011,389 - Pf6N2E2_1702 0.74 -1.2 2,011,416 - Pf6N2E2_1702 0.76 -1.5 2,011,460 + Pf6N2E2_1702 0.79 -0.1 2,011,460 + Pf6N2E2_1702 0.79 -1.2 2,011,553 + Pf6N2E2_1702 0.86 -0.2 2,011,563 - Pf6N2E2_1702 0.87 -0.2 2,011,604 + +0.1 2,011,714 - +0.7 2,011,714 - -0.2 2,011,714 - +0.9 2,012,364 - Pf6N2E2_1703 0.49 -0.1 2,012,438 + Pf6N2E2_1703 0.56 +0.4 2,012,438 + Pf6N2E2_1703 0.56 +0.6 2,012,438 + Pf6N2E2_1703 0.56 +1.6 2,012,439 - Pf6N2E2_1703 0.56 +0.8 2,012,439 - Pf6N2E2_1703 0.56 -0.7 2,012,439 - Pf6N2E2_1703 0.56 -1.1 2,012,444 + Pf6N2E2_1703 0.57 -0.2 2,012,445 - +0.6 2,012,513 + Pf6N2E2_1703 0.63 +0.5 2,012,513 + Pf6N2E2_1703 0.63 -0.4 2,012,514 - Pf6N2E2_1703 0.63 +0.4 2,012,514 - Pf6N2E2_1703 0.63 -0.1 2,012,514 - Pf6N2E2_1703 0.63 -0.7 2,012,514 - Pf6N2E2_1703 0.63 +1.2 2,012,634 - Pf6N2E2_1703 0.75 +2.3 2,012,693 + Pf6N2E2_1703 0.80 +0.5
Or see this region's nucleotide sequence