Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_3449 and Ga0059261_3450 are separated by 22 nucleotides Ga0059261_3450 and Ga0059261_3451 overlap by 4 nucleotides Ga0059261_3451 and Ga0059261_3452 are separated by 134 nucleotides Ga0059261_3452 and Ga0059261_3453 are separated by 63 nucleotides Ga0059261_3453 and Ga0059261_3454 are separated by 350 nucleotides
Ga0059261_3449: Ga0059261_3449 - G/U mismatch-specific uracil-DNA glycosylase (EC 3.2.2.-), at 3,575,010 to 3,575,513
_3449
Ga0059261_3450: Ga0059261_3450 - Predicted membrane protein, at 3,575,536 to 3,575,862
_3450
Ga0059261_3451: Ga0059261_3451 - hypothetical protein, at 3,575,859 to 3,576,281
_3451
Ga0059261_3452: Ga0059261_3452 - Cysteine synthase, at 3,576,416 to 3,577,408
_3452
Ga0059261_3453: Ga0059261_3453 - hypothetical protein, at 3,577,472 to 3,577,777
_3453
Ga0059261_3454: Ga0059261_3454 - Uncharacterized protein conserved in bacteria, at 3,578,128 to 3,578,637
_3454
Position (kb)
3576
3577
3578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3575.524 kb on + strand at 3575.524 kb on + strand at 3575.524 kb on + strand at 3575.524 kb on + strand at 3575.524 kb on + strand at 3575.524 kb on + strand at 3575.525 kb on - strand at 3575.525 kb on - strand at 3575.525 kb on - strand at 3575.525 kb on - strand at 3575.525 kb on - strand at 3575.557 kb on + strand at 3575.558 kb on - strand at 3575.575 kb on + strand, within Ga0059261_3450 at 3575.575 kb on + strand, within Ga0059261_3450 at 3575.576 kb on - strand, within Ga0059261_3450 at 3575.576 kb on - strand, within Ga0059261_3450 at 3575.576 kb on - strand, within Ga0059261_3450 at 3575.578 kb on + strand, within Ga0059261_3450 at 3575.578 kb on + strand, within Ga0059261_3450 at 3575.578 kb on + strand, within Ga0059261_3450 at 3575.578 kb on + strand, within Ga0059261_3450 at 3575.579 kb on - strand, within Ga0059261_3450 at 3575.579 kb on - strand, within Ga0059261_3450 at 3575.579 kb on - strand, within Ga0059261_3450 at 3575.579 kb on - strand, within Ga0059261_3450 at 3575.620 kb on + strand, within Ga0059261_3450 at 3575.620 kb on + strand, within Ga0059261_3450 at 3575.620 kb on + strand, within Ga0059261_3450 at 3575.620 kb on + strand, within Ga0059261_3450 at 3575.621 kb on - strand, within Ga0059261_3450 at 3575.621 kb on - strand, within Ga0059261_3450 at 3575.621 kb on - strand, within Ga0059261_3450 at 3575.621 kb on - strand at 3575.621 kb on - strand, within Ga0059261_3450 at 3575.767 kb on + strand, within Ga0059261_3450 at 3575.767 kb on + strand, within Ga0059261_3450 at 3575.768 kb on - strand, within Ga0059261_3450 at 3575.768 kb on - strand, within Ga0059261_3450 at 3575.768 kb on - strand, within Ga0059261_3450 at 3575.794 kb on + strand, within Ga0059261_3450 at 3575.794 kb on + strand, within Ga0059261_3450 at 3575.795 kb on - strand, within Ga0059261_3450 at 3575.861 kb on - strand at 3576.106 kb on + strand, within Ga0059261_3451 at 3576.107 kb on - strand, within Ga0059261_3451 at 3576.107 kb on - strand, within Ga0059261_3451 at 3576.265 kb on + strand at 3576.266 kb on - strand at 3576.266 kb on - strand at 3576.272 kb on + strand at 3576.272 kb on + strand at 3576.272 kb on + strand at 3576.272 kb on + strand at 3576.273 kb on - strand at 3576.273 kb on - strand at 3576.273 kb on - strand at 3576.506 kb on + strand at 3576.506 kb on + strand at 3576.507 kb on - strand at 3576.507 kb on - strand at 3576.507 kb on - strand at 3576.862 kb on + strand at 3577.055 kb on + strand, within Ga0059261_3452 at 3577.055 kb on + strand, within Ga0059261_3452 at 3577.055 kb on + strand, within Ga0059261_3452 at 3577.055 kb on + strand, within Ga0059261_3452 at 3577.058 kb on + strand at 3577.123 kb on + strand at 3577.346 kb on + strand at 3577.347 kb on - strand at 3577.347 kb on - strand at 3577.417 kb on + strand at 3577.417 kb on + strand at 3577.452 kb on + strand at 3577.452 kb on + strand at 3577.757 kb on + strand at 3577.758 kb on - strand at 3577.890 kb on + strand at 3577.890 kb on + strand at 3577.890 kb on + strand at 3577.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,575,524 + -1.6 3,575,524 + -0.0 3,575,524 + -0.9 3,575,524 + -0.1 3,575,524 + -0.0 3,575,524 + +1.1 3,575,525 - +0.7 3,575,525 - +0.5 3,575,525 - -0.3 3,575,525 - +0.7 3,575,525 - -0.7 3,575,557 + -1.6 3,575,558 - +1.7 3,575,575 + Ga0059261_3450 0.12 +2.0 3,575,575 + Ga0059261_3450 0.12 +0.2 3,575,576 - Ga0059261_3450 0.12 +0.5 3,575,576 - Ga0059261_3450 0.12 +0.1 3,575,576 - Ga0059261_3450 0.12 +0.4 3,575,578 + Ga0059261_3450 0.13 +1.0 3,575,578 + Ga0059261_3450 0.13 +0.0 3,575,578 + Ga0059261_3450 0.13 -0.7 3,575,578 + Ga0059261_3450 0.13 +2.1 3,575,579 - Ga0059261_3450 0.13 -0.5 3,575,579 - Ga0059261_3450 0.13 +1.1 3,575,579 - Ga0059261_3450 0.13 +0.9 3,575,579 - Ga0059261_3450 0.13 -0.3 3,575,620 + Ga0059261_3450 0.26 -2.5 3,575,620 + Ga0059261_3450 0.26 -1.6 3,575,620 + Ga0059261_3450 0.26 -1.5 3,575,620 + Ga0059261_3450 0.26 +0.3 3,575,621 - Ga0059261_3450 0.26 -1.6 3,575,621 - Ga0059261_3450 0.26 +0.8 3,575,621 - Ga0059261_3450 0.26 -1.2 3,575,621 - +1.0 3,575,621 - Ga0059261_3450 0.26 +0.2 3,575,767 + Ga0059261_3450 0.71 +1.5 3,575,767 + Ga0059261_3450 0.71 +0.3 3,575,768 - Ga0059261_3450 0.71 +0.1 3,575,768 - Ga0059261_3450 0.71 +0.4 3,575,768 - Ga0059261_3450 0.71 +2.0 3,575,794 + Ga0059261_3450 0.79 +0.5 3,575,794 + Ga0059261_3450 0.79 -0.9 3,575,795 - Ga0059261_3450 0.79 +2.0 3,575,861 - +0.1 3,576,106 + Ga0059261_3451 0.58 -1.7 3,576,107 - Ga0059261_3451 0.59 +0.7 3,576,107 - Ga0059261_3451 0.59 -0.4 3,576,265 + +0.7 3,576,266 - +0.7 3,576,266 - -0.0 3,576,272 + +0.3 3,576,272 + +1.4 3,576,272 + +1.3 3,576,272 + -0.3 3,576,273 - -1.8 3,576,273 - +1.3 3,576,273 - -0.2 3,576,506 + +0.3 3,576,506 + -0.0 3,576,507 - +0.3 3,576,507 - -0.3 3,576,507 - -0.0 3,576,862 + +1.3 3,577,055 + Ga0059261_3452 0.64 -1.3 3,577,055 + Ga0059261_3452 0.64 +1.3 3,577,055 + Ga0059261_3452 0.64 -0.3 3,577,055 + Ga0059261_3452 0.64 -1.0 3,577,058 + +0.7 3,577,123 + +1.3 3,577,346 + +0.3 3,577,347 - -1.9 3,577,347 - -0.7 3,577,417 + -3.4 3,577,417 + +5.6 3,577,452 + -0.7 3,577,452 + +0.4 3,577,757 + +1.0 3,577,758 - +2.1 3,577,890 + +0.3 3,577,890 + +0.3 3,577,890 + +0.7 3,577,922 - +6.4
Or see this region's nucleotide sequence