Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_1526 and Ga0059261_1527 are separated by 20 nucleotides Ga0059261_1527 and Ga0059261_1528 are separated by 201 nucleotides Ga0059261_1528 and Ga0059261_1529 overlap by 4 nucleotides Ga0059261_1529 and Ga0059261_1530 overlap by 1 nucleotides Ga0059261_1530 and Ga0059261_1531 are separated by 186 nucleotides
Ga0059261_1526: Ga0059261_1526 - phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 1,606,025 to 1,606,807
_1526
Ga0059261_1527: Ga0059261_1527 - hypothetical protein, at 1,606,828 to 1,606,977
_1527
Ga0059261_1528: Ga0059261_1528 - phosphoribosylformylglycinamidine synthase, purS protein, at 1,607,179 to 1,607,424
_1528
Ga0059261_1529: Ga0059261_1529 - phosphoribosylformylglycinamidine synthase I, at 1,607,421 to 1,608,086
_1529
Ga0059261_1530: Ga0059261_1530 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 1,608,086 to 1,608,784
_1530
Ga0059261_1531: Ga0059261_1531 - hypothetical protein, at 1,608,971 to 1,609,375
_1531
Position (kb)
1607
1608
1609 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1606.805 kb on + strand at 1606.805 kb on + strand at 1606.805 kb on + strand at 1606.805 kb on + strand at 1606.805 kb on + strand at 1606.805 kb on + strand at 1606.805 kb on + strand at 1606.806 kb on - strand at 1606.806 kb on - strand at 1606.806 kb on - strand at 1606.806 kb on - strand at 1606.806 kb on - strand at 1606.896 kb on + strand, within Ga0059261_1527 at 1606.896 kb on + strand, within Ga0059261_1527 at 1606.897 kb on - strand, within Ga0059261_1527 at 1606.897 kb on - strand, within Ga0059261_1527 at 1606.980 kb on + strand at 1606.980 kb on + strand at 1606.980 kb on + strand at 1606.980 kb on + strand at 1606.981 kb on - strand at 1606.981 kb on - strand at 1607.052 kb on - strand at 1607.147 kb on + strand at 1608.203 kb on + strand, within Ga0059261_1530 at 1608.205 kb on + strand, within Ga0059261_1530 at 1608.205 kb on + strand, within Ga0059261_1530 at 1608.205 kb on + strand, within Ga0059261_1530 at 1608.206 kb on - strand, within Ga0059261_1530 at 1608.206 kb on - strand, within Ga0059261_1530 at 1608.206 kb on - strand, within Ga0059261_1530 at 1608.206 kb on - strand, within Ga0059261_1530 at 1608.747 kb on + strand at 1608.747 kb on + strand at 1608.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,606,805 + +0.5 1,606,805 + +0.5 1,606,805 + -0.3 1,606,805 + -2.1 1,606,805 + -2.8 1,606,805 + -1.1 1,606,805 + -0.3 1,606,806 - -1.5 1,606,806 - +0.2 1,606,806 - -2.3 1,606,806 - -0.3 1,606,806 - -0.8 1,606,896 + Ga0059261_1527 0.45 +0.3 1,606,896 + Ga0059261_1527 0.45 +0.5 1,606,897 - Ga0059261_1527 0.46 +0.6 1,606,897 - Ga0059261_1527 0.46 +0.8 1,606,980 + -0.1 1,606,980 + -1.1 1,606,980 + +1.1 1,606,980 + -1.4 1,606,981 - +2.1 1,606,981 - +0.3 1,607,052 - -1.5 1,607,147 + +1.5 1,608,203 + Ga0059261_1530 0.17 -1.9 1,608,205 + Ga0059261_1530 0.17 -0.2 1,608,205 + Ga0059261_1530 0.17 -1.0 1,608,205 + Ga0059261_1530 0.17 -2.0 1,608,206 - Ga0059261_1530 0.17 +1.2 1,608,206 - Ga0059261_1530 0.17 -2.4 1,608,206 - Ga0059261_1530 0.17 -2.0 1,608,206 - Ga0059261_1530 0.17 -1.0 1,608,747 + +2.2 1,608,747 + +0.7 1,608,748 - -2.1
Or see this region's nucleotide sequence