Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_0625 and Ga0059261_0626 overlap by 4 nucleotides Ga0059261_0626 and Ga0059261_0627 overlap by 4 nucleotides Ga0059261_0627 and Ga0059261_0628 overlap by 4 nucleotides
Ga0059261_0625: Ga0059261_0625 - nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 665,151 to 666,164
_0625
Ga0059261_0626: Ga0059261_0626 - Fructose-2,6-bisphosphatase, at 666,161 to 666,730
_0626
Ga0059261_0627: Ga0059261_0627 - cobalamin-5'-phosphate synthase (EC 2.7.8.26), at 666,727 to 667,443
_0627
Ga0059261_0628: Ga0059261_0628 - L-threonine O-3-phosphate decarboxylase (EC 4.1.1.81), at 667,440 to 668,399
_0628
Position (kb) 666
667 Strain fitness (log2 ratio) -2
-1
0
1
2 at 665.648 kb on + strand, within Ga0059261_0625 at 665.648 kb on + strand, within Ga0059261_0625 at 665.648 kb on + strand, within Ga0059261_0625 at 665.648 kb on + strand, within Ga0059261_0625 at 665.648 kb on + strand, within Ga0059261_0625 at 665.649 kb on - strand, within Ga0059261_0625 at 665.649 kb on - strand, within Ga0059261_0625 at 665.738 kb on + strand, within Ga0059261_0625 at 666.179 kb on + strand at 666.179 kb on + strand at 666.180 kb on - strand at 666.180 kb on - strand at 666.180 kb on - strand at 666.180 kb on - strand at 666.180 kb on - strand at 666.180 kb on - strand at 666.256 kb on + strand, within Ga0059261_0626 at 666.257 kb on - strand, within Ga0059261_0626 at 666.257 kb on - strand, within Ga0059261_0626 at 666.656 kb on + strand, within Ga0059261_0626 at 666.656 kb on + strand, within Ga0059261_0626 at 666.657 kb on - strand, within Ga0059261_0626 at 666.885 kb on + strand, within Ga0059261_0627 at 667.635 kb on + strand, within Ga0059261_0628 at 667.636 kb on - strand at 667.728 kb on + strand, within Ga0059261_0628
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 665,648 + Ga0059261_0625 0.49 -1.1 665,648 + Ga0059261_0625 0.49 +2.0 665,648 + Ga0059261_0625 0.49 +0.5 665,648 + Ga0059261_0625 0.49 +0.2 665,648 + Ga0059261_0625 0.49 +1.4 665,649 - Ga0059261_0625 0.49 -1.4 665,649 - Ga0059261_0625 0.49 +0.6 665,738 + Ga0059261_0625 0.58 -0.2 666,179 + -1.1 666,179 + +1.6 666,180 - -0.6 666,180 - +0.6 666,180 - -0.2 666,180 - -0.9 666,180 - -0.8 666,180 - +1.4 666,256 + Ga0059261_0626 0.17 -1.8 666,257 - Ga0059261_0626 0.17 -0.9 666,257 - Ga0059261_0626 0.17 +0.9 666,656 + Ga0059261_0626 0.87 +1.3 666,656 + Ga0059261_0626 0.87 +0.1 666,657 - Ga0059261_0626 0.87 -0.4 666,885 + Ga0059261_0627 0.22 -1.3 667,635 + Ga0059261_0628 0.20 -0.3 667,636 - +2.2 667,728 + Ga0059261_0628 0.30 +0.7
Or see this region's nucleotide sequence