Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_0227 and Ga0059261_0228 are separated by 62 nucleotides Ga0059261_0228 and Ga0059261_0229 are separated by 120 nucleotides Ga0059261_0229 and Ga0059261_0230 overlap by 4 nucleotides
Ga0059261_0227: Ga0059261_0227 - hypothetical protein, at 217,675 to 218,949
_0227
Ga0059261_0228: Ga0059261_0228 - phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 219,012 to 220,106
_0228
Ga0059261_0229: Ga0059261_0229 - phosphoribosylglycinamide formyltransferase, formyltetrahydrofolate-dependent, at 220,227 to 220,793
_0229
Ga0059261_0230: Ga0059261_0230 - Uncharacterized protein conserved in bacteria, at 220,790 to 221,167
_0230
Position (kb) 219
220
221 Strain fitness (log2 ratio) -2
-1
0
1
2 at 218.115 kb on - strand, within Ga0059261_0227 at 218.115 kb on - strand at 218.115 kb on - strand, within Ga0059261_0227 at 218.406 kb on - strand, within Ga0059261_0227 at 218.406 kb on - strand, within Ga0059261_0227 at 218.938 kb on - strand at 218.938 kb on - strand at 220.097 kb on + strand at 220.097 kb on + strand at 220.097 kb on + strand at 220.097 kb on + strand at 220.097 kb on + strand at 220.098 kb on - strand at 220.098 kb on - strand at 220.098 kb on - strand at 220.111 kb on + strand at 220.111 kb on + strand at 220.112 kb on - strand at 220.315 kb on - strand, within Ga0059261_0229 at 220.428 kb on + strand, within Ga0059261_0229 at 220.429 kb on - strand, within Ga0059261_0229 at 220.429 kb on - strand, within Ga0059261_0229 at 220.429 kb on - strand, within Ga0059261_0229 at 220.491 kb on + strand, within Ga0059261_0229 at 220.491 kb on + strand, within Ga0059261_0229 at 220.491 kb on + strand, within Ga0059261_0229 at 220.493 kb on + strand at 220.493 kb on + strand, within Ga0059261_0229 at 220.494 kb on - strand, within Ga0059261_0229 at 220.494 kb on - strand, within Ga0059261_0229 at 220.547 kb on + strand, within Ga0059261_0229 at 220.572 kb on + strand, within Ga0059261_0229 at 220.572 kb on + strand at 220.573 kb on - strand, within Ga0059261_0229 at 220.573 kb on - strand at 220.573 kb on - strand, within Ga0059261_0229 at 220.573 kb on - strand, within Ga0059261_0229 at 220.573 kb on - strand at 220.573 kb on - strand, within Ga0059261_0229 at 220.573 kb on - strand, within Ga0059261_0229 at 220.759 kb on - strand at 220.916 kb on + strand, within Ga0059261_0230 at 220.917 kb on - strand, within Ga0059261_0230 at 221.018 kb on + strand, within Ga0059261_0230 at 221.018 kb on + strand, within Ga0059261_0230 at 221.018 kb on + strand, within Ga0059261_0230 at 221.019 kb on - strand, within Ga0059261_0230 at 221.019 kb on - strand, within Ga0059261_0230 at 221.019 kb on - strand, within Ga0059261_0230 at 221.021 kb on + strand, within Ga0059261_0230 at 221.022 kb on - strand, within Ga0059261_0230 at 221.033 kb on + strand, within Ga0059261_0230 at 221.033 kb on + strand, within Ga0059261_0230 at 221.033 kb on + strand, within Ga0059261_0230
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 218,115 - Ga0059261_0227 0.35 -0.6 218,115 - +1.3 218,115 - Ga0059261_0227 0.35 -2.2 218,406 - Ga0059261_0227 0.57 -1.9 218,406 - Ga0059261_0227 0.57 -1.3 218,938 - +0.7 218,938 - -1.2 220,097 + -0.5 220,097 + -0.7 220,097 + +0.3 220,097 + +0.3 220,097 + +0.3 220,098 - -0.7 220,098 - -0.6 220,098 - -2.6 220,111 + +1.3 220,111 + +2.3 220,112 - -0.3 220,315 - Ga0059261_0229 0.16 -0.4 220,428 + Ga0059261_0229 0.35 +0.4 220,429 - Ga0059261_0229 0.36 -0.0 220,429 - Ga0059261_0229 0.36 -0.0 220,429 - Ga0059261_0229 0.36 -2.0 220,491 + Ga0059261_0229 0.47 -0.2 220,491 + Ga0059261_0229 0.47 -0.3 220,491 + Ga0059261_0229 0.47 -2.4 220,493 + +0.7 220,493 + Ga0059261_0229 0.47 +2.0 220,494 - Ga0059261_0229 0.47 +0.7 220,494 - Ga0059261_0229 0.47 -0.3 220,547 + Ga0059261_0229 0.56 +1.2 220,572 + Ga0059261_0229 0.61 +0.7 220,572 + +0.7 220,573 - Ga0059261_0229 0.61 -0.7 220,573 - +0.3 220,573 - Ga0059261_0229 0.61 -0.5 220,573 - Ga0059261_0229 0.61 -1.7 220,573 - +0.3 220,573 - Ga0059261_0229 0.61 -1.4 220,573 - Ga0059261_0229 0.61 -0.5 220,759 - +0.7 220,916 + Ga0059261_0230 0.33 -0.3 220,917 - Ga0059261_0230 0.34 -1.5 221,018 + Ga0059261_0230 0.60 -1.3 221,018 + Ga0059261_0230 0.60 +2.0 221,018 + Ga0059261_0230 0.60 +0.8 221,019 - Ga0059261_0230 0.61 +2.3 221,019 - Ga0059261_0230 0.61 -0.2 221,019 - Ga0059261_0230 0.61 -1.4 221,021 + Ga0059261_0230 0.61 -1.3 221,022 - Ga0059261_0230 0.61 +1.4 221,033 + Ga0059261_0230 0.64 +1.6 221,033 + Ga0059261_0230 0.64 -2.0 221,033 + Ga0059261_0230 0.64 -1.2
Or see this region's nucleotide sequence