Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3340 and Shew_3341 are separated by 75 nucleotides Shew_3341 and Shew_3342 are separated by 200 nucleotides Shew_3342 and Shew_3343 overlap by 4 nucleotides
Shew_3340: Shew_3340 - TonB-dependent receptor (RefSeq), at 3,983,615 to 3,985,672
_3340
Shew_3341: Shew_3341 - diguanylate cyclase with GAF sensor (RefSeq), at 3,985,748 to 3,986,728
_3341
Shew_3342: Shew_3342 - two component Fis family transcriptional regulator (RefSeq), at 3,986,929 to 3,987,465
_3342
Shew_3343: Shew_3343 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 3,987,462 to 3,988,709
_3343
Position (kb)
3985
3986
3987 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3984.940 kb on + strand, within Shew_3340 at 3984.951 kb on + strand, within Shew_3340 at 3985.440 kb on - strand, within Shew_3340 at 3985.488 kb on + strand at 3985.488 kb on + strand at 3985.693 kb on + strand at 3985.778 kb on + strand at 3985.788 kb on - strand at 3985.887 kb on + strand, within Shew_3341 at 3986.541 kb on + strand, within Shew_3341 at 3986.588 kb on + strand, within Shew_3341 at 3986.711 kb on - strand at 3986.836 kb on - strand at 3986.836 kb on - strand at 3986.949 kb on - strand at 3987.043 kb on + strand, within Shew_3342 at 3987.352 kb on - strand, within Shew_3342 at 3987.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 3,984,940 + Shew_3340 0.64 +0.5 3,984,951 + Shew_3340 0.65 -0.8 3,985,440 - Shew_3340 0.89 +0.1 3,985,488 + -0.2 3,985,488 + -0.0 3,985,693 + +0.5 3,985,778 + -1.3 3,985,788 - -1.2 3,985,887 + Shew_3341 0.14 +0.5 3,986,541 + Shew_3341 0.81 +1.1 3,986,588 + Shew_3341 0.86 -0.0 3,986,711 - -0.2 3,986,836 - -3.2 3,986,836 - +0.1 3,986,949 - +1.1 3,987,043 + Shew_3342 0.21 +0.4 3,987,352 - Shew_3342 0.79 +0.3 3,987,523 - +0.0
Or see this region's nucleotide sequence