Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2257 and Shew_2258 are separated by 93 nucleotides Shew_2258 and Shew_2259 are separated by 159 nucleotides Shew_2259 and Shew_2260 are separated by 386 nucleotides Shew_2260 and Shew_2261 overlap by 1 nucleotides
Shew_2257: Shew_2257 - HAD family hydrolase (RefSeq), at 2,637,166 to 2,637,789
_2257
Shew_2258: Shew_2258 - NADH:ubiquinone oxidoreductase complex I intermediate-associated protein 30 (RefSeq), at 2,637,883 to 2,638,413
_2258
Shew_2259: Shew_2259 - metal dependent phosphohydrolase (RefSeq), at 2,638,573 to 2,639,775
_2259
Shew_2260: Shew_2260 - intracellular septation protein A (RefSeq), at 2,640,162 to 2,640,707
_2260
Shew_2261: Shew_2261 - YciI-like protein (RefSeq), at 2,640,707 to 2,641,006
_2261
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2637.749 kb on - strand at 2637.761 kb on - strand at 2637.761 kb on - strand at 2637.778 kb on - strand at 2637.845 kb on + strand at 2637.853 kb on - strand at 2638.223 kb on + strand, within Shew_2258 at 2638.787 kb on + strand, within Shew_2259 at 2638.883 kb on - strand, within Shew_2259 at 2639.353 kb on - strand, within Shew_2259 at 2639.580 kb on - strand, within Shew_2259 at 2639.658 kb on - strand at 2640.032 kb on - strand at 2640.671 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 2,637,749 - +1.6 2,637,761 - -0.4 2,637,761 - -0.3 2,637,778 - +0.1 2,637,845 + -3.6 2,637,853 - -0.8 2,638,223 + Shew_2258 0.64 -0.2 2,638,787 + Shew_2259 0.18 -1.2 2,638,883 - Shew_2259 0.26 -2.2 2,639,353 - Shew_2259 0.65 -1.4 2,639,580 - Shew_2259 0.84 -1.4 2,639,658 - -1.1 2,640,032 - -0.6 2,640,671 + -0.0
Or see this region's nucleotide sequence