Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0228 and rnpB are separated by 109 nucleotides rnpB and Shew_0229 are separated by 96 nucleotides Shew_0229 and Shew_0230 are separated by 69 nucleotides
Shew_0228: Shew_0228 - uroporphyrin-III C/tetrapyrrole methyltransferase (RefSeq), at 241,543 to 242,385
_0228
Shew_R0013: rnpB - RNA component of RNaseP (NCBI), at 242,495 to 242,800
rnpB
Shew_0229: Shew_0229 - extracellular solute-binding protein (RefSeq), at 242,897 to 243,706
_0229
Shew_0230: Shew_0230 - hypothetical protein (RefSeq), at 243,776 to 245,485
_0230
Position (kb) 242
243
244 Strain fitness (log2 ratio) -1
0
1 at 241.921 kb on - strand, within Shew_0228 at 242.158 kb on - strand, within Shew_0228 at 242.233 kb on + strand, within Shew_0228 at 242.331 kb on - strand at 242.416 kb on + strand at 242.904 kb on + strand at 243.030 kb on - strand, within Shew_0229 at 243.590 kb on + strand, within Shew_0229 at 243.598 kb on - strand, within Shew_0229 at 243.762 kb on + strand at 243.770 kb on - strand at 243.845 kb on + strand at 243.858 kb on - strand at 243.867 kb on - strand at 244.107 kb on - strand, within Shew_0230 at 244.165 kb on + strand, within Shew_0230 at 244.173 kb on - strand, within Shew_0230 at 244.607 kb on + strand, within Shew_0230
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 241,921 - Shew_0228 0.45 -0.1 242,158 - Shew_0228 0.73 +1.7 242,233 + Shew_0228 0.82 -0.6 242,331 - +0.4 242,416 + +0.2 242,904 + +0.1 243,030 - Shew_0229 0.16 +1.0 243,590 + Shew_0229 0.86 +0.6 243,598 - Shew_0229 0.87 +1.2 243,762 + -0.7 243,770 - -0.2 243,845 + -0.8 243,858 - -0.5 243,867 - +0.3 244,107 - Shew_0230 0.19 +0.3 244,165 + Shew_0230 0.23 -0.2 244,173 - Shew_0230 0.23 +0.4 244,607 + Shew_0230 0.49 -0.8
Or see this region's nucleotide sequence