Experiment: m.b. Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4198 and Dshi_4199 are separated by 65 nucleotides Dshi_4199 and Dshi_4200 are separated by 145 nucleotides Dshi_4200 and Dshi_4201 overlap by 4 nucleotides
Dshi_4198: Dshi_4198 - transcriptional regulator, LysR family (RefSeq), at 44,976 to 45,887
_4198
Dshi_4199: Dshi_4199 - transcriptional regulator, TetR family (RefSeq), at 45,953 to 46,597
_4199
Dshi_4200: Dshi_4200 - Carbon-monoxide dehydrogenase (acceptor) (RefSeq), at 46,743 to 47,576
_4200
Dshi_4201: Dshi_4201 - (2Fe-2S)-binding domain protein (RefSeq), at 47,573 to 48,073
_4201
Position (kb) 45
46
47 Strain fitness (log2 ratio) -1
0
1 at 45.146 kb on - strand, within Dshi_4198 at 45.189 kb on + strand, within Dshi_4198 at 45.225 kb on + strand, within Dshi_4198 at 45.278 kb on - strand, within Dshi_4198 at 45.470 kb on - strand, within Dshi_4198 at 45.820 kb on + strand at 45.882 kb on + strand at 46.188 kb on - strand, within Dshi_4199 at 46.299 kb on - strand, within Dshi_4199 at 46.322 kb on + strand, within Dshi_4199 at 46.771 kb on + strand at 46.982 kb on + strand, within Dshi_4200 at 47.297 kb on + strand, within Dshi_4200 at 47.316 kb on - strand, within Dshi_4200 at 47.331 kb on + strand, within Dshi_4200 at 47.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.004 mg/ml remove 45,146 - Dshi_4198 0.19 +0.1 45,189 + Dshi_4198 0.23 +1.3 45,225 + Dshi_4198 0.27 -0.8 45,278 - Dshi_4198 0.33 -0.3 45,470 - Dshi_4198 0.54 -0.6 45,820 + -1.1 45,882 + -0.7 46,188 - Dshi_4199 0.36 -0.2 46,299 - Dshi_4199 0.54 -1.1 46,322 + Dshi_4199 0.57 +0.4 46,771 + -0.9 46,982 + Dshi_4200 0.29 -0.1 47,297 + Dshi_4200 0.66 -1.3 47,316 - Dshi_4200 0.69 -0.5 47,331 + Dshi_4200 0.71 -0.7 47,589 - -0.0
Or see this region's nucleotide sequence