Experiment: m.b. Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3531 and Dshi_3532 are separated by 42 nucleotides Dshi_3532 and Dshi_3533 are separated by 257 nucleotides Dshi_3533 and Dshi_3534 overlap by 4 nucleotides
Dshi_3531: Dshi_3531 - transcriptional regulator, Fis family (RefSeq), at 3,711,054 to 3,712,484
_3531
Dshi_3532: Dshi_3532 - cobalamin B12-binding domain protein (RefSeq), at 3,712,527 to 3,713,387
_3532
Dshi_3533: Dshi_3533 - 2-vinyl bacteriochlorophyllide hydratase (RefSeq), at 3,713,645 to 3,714,133
_3533
Dshi_3534: Dshi_3534 - light-independent protochlorophyllide reductase, N subunit (RefSeq), at 3,714,130 to 3,715,413
_3534
Position (kb)
3712
3713
3714 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3711.769 kb on - strand, within Dshi_3531 at 3711.820 kb on + strand, within Dshi_3531 at 3711.856 kb on - strand, within Dshi_3531 at 3712.361 kb on - strand at 3712.643 kb on - strand, within Dshi_3532 at 3712.643 kb on - strand, within Dshi_3532 at 3712.743 kb on - strand, within Dshi_3532 at 3712.768 kb on - strand, within Dshi_3532 at 3712.775 kb on + strand, within Dshi_3532 at 3712.792 kb on + strand, within Dshi_3532 at 3712.868 kb on + strand, within Dshi_3532 at 3712.878 kb on + strand, within Dshi_3532 at 3713.013 kb on + strand, within Dshi_3532 at 3713.029 kb on + strand, within Dshi_3532 at 3713.049 kb on - strand, within Dshi_3532 at 3713.123 kb on - strand, within Dshi_3532 at 3713.166 kb on - strand, within Dshi_3532 at 3713.294 kb on + strand, within Dshi_3532 at 3713.302 kb on - strand at 3713.302 kb on - strand at 3713.327 kb on + strand at 3713.342 kb on + strand at 3713.350 kb on - strand at 3713.360 kb on - strand at 3713.360 kb on - strand at 3713.371 kb on - strand at 3713.417 kb on - strand at 3713.455 kb on + strand at 3713.538 kb on + strand at 3713.544 kb on + strand at 3713.555 kb on + strand at 3713.601 kb on - strand at 3713.676 kb on + strand at 3713.694 kb on - strand, within Dshi_3533 at 3713.821 kb on - strand, within Dshi_3533 at 3713.826 kb on - strand, within Dshi_3533 at 3713.856 kb on - strand, within Dshi_3533 at 3713.863 kb on + strand, within Dshi_3533 at 3713.871 kb on - strand, within Dshi_3533 at 3713.946 kb on - strand, within Dshi_3533 at 3713.948 kb on - strand, within Dshi_3533 at 3713.989 kb on + strand, within Dshi_3533 at 3714.017 kb on - strand, within Dshi_3533 at 3714.132 kb on + strand at 3714.183 kb on - strand at 3714.187 kb on - strand at 3714.250 kb on - strand at 3714.329 kb on - strand, within Dshi_3534 at 3714.370 kb on - strand, within Dshi_3534
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.004 mg/ml remove 3,711,769 - Dshi_3531 0.50 -4.7 3,711,820 + Dshi_3531 0.54 -1.0 3,711,856 - Dshi_3531 0.56 -1.8 3,712,361 - -2.7 3,712,643 - Dshi_3532 0.13 +0.0 3,712,643 - Dshi_3532 0.13 +1.3 3,712,743 - Dshi_3532 0.25 +1.3 3,712,768 - Dshi_3532 0.28 +0.2 3,712,775 + Dshi_3532 0.29 -2.8 3,712,792 + Dshi_3532 0.31 +0.6 3,712,868 + Dshi_3532 0.40 -0.6 3,712,878 + Dshi_3532 0.41 +2.3 3,713,013 + Dshi_3532 0.56 +0.1 3,713,029 + Dshi_3532 0.58 -0.5 3,713,049 - Dshi_3532 0.61 +2.4 3,713,123 - Dshi_3532 0.69 +0.3 3,713,166 - Dshi_3532 0.74 -0.1 3,713,294 + Dshi_3532 0.89 -0.0 3,713,302 - +0.4 3,713,302 - +1.0 3,713,327 + -0.8 3,713,342 + +0.6 3,713,350 - +1.0 3,713,360 - +0.3 3,713,360 - -0.1 3,713,371 - +0.5 3,713,417 - +0.2 3,713,455 + +0.1 3,713,538 + -0.7 3,713,544 + +0.2 3,713,555 + +0.0 3,713,601 - +1.0 3,713,676 + -0.8 3,713,694 - Dshi_3533 0.10 -1.0 3,713,821 - Dshi_3533 0.36 -0.5 3,713,826 - Dshi_3533 0.37 -0.8 3,713,856 - Dshi_3533 0.43 -1.8 3,713,863 + Dshi_3533 0.45 +0.7 3,713,871 - Dshi_3533 0.46 -0.7 3,713,946 - Dshi_3533 0.62 -1.2 3,713,948 - Dshi_3533 0.62 -0.3 3,713,989 + Dshi_3533 0.70 -0.1 3,714,017 - Dshi_3533 0.76 -1.2 3,714,132 + +0.2 3,714,183 - -0.8 3,714,187 - +0.7 3,714,250 - -0.6 3,714,329 - Dshi_3534 0.15 +0.7 3,714,370 - Dshi_3534 0.19 -0.2
Or see this region's nucleotide sequence