Experiment: m.b. Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2132 and Dshi_2133 overlap by 4 nucleotides Dshi_2133 and Dshi_2134 are separated by 209 nucleotides Dshi_2134 and Dshi_2135 are separated by 122 nucleotides Dshi_2135 and Dshi_2136 are separated by 99 nucleotides
Dshi_2132: Dshi_2132 - amidophosphoribosyltransferase (RefSeq), at 2,262,378 to 2,263,904
_2132
Dshi_2133: Dshi_2133 - protein of unknown function DUF1284 (RefSeq), at 2,263,901 to 2,264,419
_2133
Dshi_2134: Dshi_2134 - BioY protein (RefSeq), at 2,264,629 to 2,265,222
_2134
Dshi_2135: Dshi_2135 - short-chain dehydrogenase/reductase SDR (RefSeq), at 2,265,345 to 2,266,010
_2135
Dshi_2136: Dshi_2136 - stationary-phase survival protein SurE (RefSeq), at 2,266,110 to 2,266,895
_2136
Position (kb)
2264
2265
2266 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2263.637 kb on - strand, within Dshi_2132 at 2263.657 kb on + strand, within Dshi_2132 at 2263.722 kb on + strand, within Dshi_2132 at 2263.765 kb on - strand at 2263.863 kb on - strand at 2263.876 kb on + strand at 2263.942 kb on - strand at 2263.962 kb on - strand, within Dshi_2133 at 2264.056 kb on - strand, within Dshi_2133 at 2264.076 kb on + strand, within Dshi_2133 at 2264.178 kb on + strand, within Dshi_2133 at 2264.317 kb on - strand, within Dshi_2133 at 2264.449 kb on + strand at 2264.449 kb on + strand at 2264.569 kb on + strand at 2264.813 kb on + strand, within Dshi_2134 at 2265.205 kb on - strand at 2265.323 kb on - strand at 2265.329 kb on + strand at 2265.455 kb on + strand, within Dshi_2135 at 2266.051 kb on + strand at 2266.089 kb on + strand at 2266.101 kb on + strand at 2266.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.004 mg/ml remove 2,263,637 - Dshi_2132 0.82 +0.2 2,263,657 + Dshi_2132 0.84 +0.1 2,263,722 + Dshi_2132 0.88 +0.6 2,263,765 - -0.4 2,263,863 - +0.7 2,263,876 + -0.3 2,263,942 - +0.5 2,263,962 - Dshi_2133 0.12 -0.4 2,264,056 - Dshi_2133 0.30 -0.2 2,264,076 + Dshi_2133 0.34 +0.4 2,264,178 + Dshi_2133 0.53 +0.2 2,264,317 - Dshi_2133 0.80 -0.2 2,264,449 + +0.7 2,264,449 + -0.3 2,264,569 + -0.4 2,264,813 + Dshi_2134 0.31 -0.2 2,265,205 - -2.9 2,265,323 - +0.0 2,265,329 + -0.0 2,265,455 + Dshi_2135 0.17 +0.5 2,266,051 + -1.9 2,266,089 + +1.0 2,266,101 + -1.0 2,266,106 + -1.5
Or see this region's nucleotide sequence