Experiment: Hydrochloric Acid 0.005 M 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_5544 and Pf6N2E2_5545 are separated by 166 nucleotides Pf6N2E2_5545 and Pf6N2E2_5546 are separated by 1 nucleotides Pf6N2E2_5546 and Pf6N2E2_6157 are separated by 63 nucleotides Pf6N2E2_6157 and Pf6N2E2_5547 are separated by 152 nucleotides 
        Pf6N2E2_5544 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 6,358,942 to 6,359,706 
        _5544 
         
        
        Pf6N2E2_5545 - Queuosine Biosynthesis QueE Radical SAM, at 6,359,873 to 6,360,520 
        _5545 
         
        
        Pf6N2E2_5546 - Queuosine Biosynthesis QueC ATPase, at 6,360,522 to 6,361,214 
        _5546 
         
        
        Pf6N2E2_6157 - tRNA-Lys-TTT, at 6,361,278 to 6,361,350 
        _6157 
         
        
        Pf6N2E2_5547 - hypothetical protein, at 6,361,503 to 6,362,933 
        _5547 
         Position (kb) 6360 
6361 
6362 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.575 kb on + strand, within Pf6N2E2_5544 at 6359.591 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.705 kb on - strand at 6359.705 kb on - strand at 6359.777 kb on - strand at 6359.850 kb on + strand at 6359.857 kb on + strand at 6359.858 kb on - strand at 6359.873 kb on - strand at 6359.949 kb on + strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.987 kb on + strand, within Pf6N2E2_5545 at 6360.063 kb on - strand, within Pf6N2E2_5545 at 6360.074 kb on + strand, within Pf6N2E2_5545 at 6360.075 kb on - strand, within Pf6N2E2_5545 at 6360.188 kb on - strand, within Pf6N2E2_5545 at 6360.362 kb on + strand, within Pf6N2E2_5545 at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.488 kb on - strand at 6360.488 kb on - strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.492 kb on - strand at 6360.521 kb on + strand at 6360.521 kb on + strand at 6360.522 kb on - strand at 6360.522 kb on - strand at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.649 kb on - strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.841 kb on + strand, within Pf6N2E2_5546 at 6360.842 kb on - strand, within Pf6N2E2_5546 at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.425 kb on - strand at 6361.668 kb on - strand, within Pf6N2E2_5547 at 6361.746 kb on + strand, within Pf6N2E2_5547 at 6361.746 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.769 kb on - strand, within Pf6N2E2_5547 at 6361.769 kb on - strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.852 kb on - strand, within Pf6N2E2_5547 at 6361.852 kb on - strand, within Pf6N2E2_5547 at 6361.918 kb on + strand, within Pf6N2E2_5547 at 6361.919 kb on - strand, within Pf6N2E2_5547 at 6361.950 kb on + strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6362.008 kb on + strand, within Pf6N2E2_5547 at 6362.008 kb on + strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.034 kb on + strand, within Pf6N2E2_5547 at 6362.034 kb on + strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.089 kb on + strand, within Pf6N2E2_5547 at 6362.089 kb on + strand, within Pf6N2E2_5547 at 6362.090 kb on - strand, within Pf6N2E2_5547 at 6362.090 kb on - strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.175 kb on + strand, within Pf6N2E2_5547  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Hydrochloric Acid 0.005 M remove 6,359,543 -  Pf6N2E2_5544 0.79  +1.0 6,359,543 -  Pf6N2E2_5544 0.79  +0.4 6,359,543 -  Pf6N2E2_5544 0.79  +0.1 6,359,543 -  Pf6N2E2_5544 0.79  -0.1 6,359,575 +  Pf6N2E2_5544 0.83  +1.0 6,359,591 +  Pf6N2E2_5544 0.85  +0.3 6,359,621 +  Pf6N2E2_5544 0.89  -0.2 6,359,621 +  Pf6N2E2_5544 0.89  +0.4 6,359,621 +  Pf6N2E2_5544 0.89  -0.2 6,359,621 +  Pf6N2E2_5544 0.89  -0.5 6,359,622 -  Pf6N2E2_5544 0.89  +0.3 6,359,622 -  Pf6N2E2_5544 0.89  +2.0 6,359,622 -  Pf6N2E2_5544 0.89  +0.9 6,359,704 +  -0.2 6,359,704 +  -0.0 6,359,704 +  +0.5 6,359,704 +  -0.2 6,359,705 -  -0.3 6,359,705 -  +0.8 6,359,777 -  +0.2 6,359,850 +  -0.7 6,359,857 +  +0.2 6,359,858 -  +0.3 6,359,873 -  -0.1 6,359,949 +  Pf6N2E2_5545 0.12  +0.3 6,359,950 -  Pf6N2E2_5545 0.12  +1.5 6,359,950 -  Pf6N2E2_5545 0.12  +0.2 6,359,987 +  Pf6N2E2_5545 0.18  -0.2 6,360,063 -  Pf6N2E2_5545 0.29  +1.4 6,360,074 +  Pf6N2E2_5545 0.31  +0.4 6,360,075 -  Pf6N2E2_5545 0.31  -0.3 6,360,188 -  Pf6N2E2_5545 0.49  +0.0 6,360,362 +  Pf6N2E2_5545 0.75  +0.3 6,360,487 +  +0.2 6,360,487 +  -0.2 6,360,487 +  +0.6 6,360,488 -  +0.5 6,360,488 -  -0.4 6,360,491 +  -0.3 6,360,491 +  -0.0 6,360,491 +  -0.5 6,360,492 -  +0.0 6,360,521 +  -1.1 6,360,521 +  +0.2 6,360,522 -  -0.5 6,360,522 -  -0.4 6,360,631 -  Pf6N2E2_5546 0.16  +0.3 6,360,631 -  Pf6N2E2_5546 0.16  -0.3 6,360,631 -  Pf6N2E2_5546 0.16  +0.0 6,360,649 -  Pf6N2E2_5546 0.18  +1.0 6,360,819 +  Pf6N2E2_5546 0.43  -1.4 6,360,819 +  Pf6N2E2_5546 0.43  -1.4 6,360,819 +  Pf6N2E2_5546 0.43  -1.5 6,360,819 +  Pf6N2E2_5546 0.43  -0.3 6,360,819 +  Pf6N2E2_5546 0.43  -0.5 6,360,819 +  Pf6N2E2_5546 0.43  +1.0 6,360,819 +  Pf6N2E2_5546 0.43  -1.4 6,360,819 +  Pf6N2E2_5546 0.43  -0.5 6,360,819 +  Pf6N2E2_5546 0.43  +0.7 6,360,819 +  Pf6N2E2_5546 0.43  -0.3 6,360,819 +  Pf6N2E2_5546 0.43  +0.2 6,360,820 -  Pf6N2E2_5546 0.43  -0.2 6,360,820 -  Pf6N2E2_5546 0.43  -1.4 6,360,820 -  Pf6N2E2_5546 0.43  -1.9 6,360,820 -  Pf6N2E2_5546 0.43  -0.4 6,360,820 -  Pf6N2E2_5546 0.43  -0.1 6,360,820 -  Pf6N2E2_5546 0.43  +0.4 6,360,833 +  Pf6N2E2_5546 0.45  -0.6 6,360,833 +  Pf6N2E2_5546 0.45  -1.3 6,360,841 +  Pf6N2E2_5546 0.46  -0.5 6,360,842 -  Pf6N2E2_5546 0.46  -0.5 6,361,424 +  +0.8 6,361,424 +  -0.7 6,361,424 +  -0.0 6,361,425 -  +0.9 6,361,668 -  Pf6N2E2_5547 0.12  +0.0 6,361,746 +  Pf6N2E2_5547 0.17  -0.4 6,361,746 +  Pf6N2E2_5547 0.17  +0.5 6,361,752 +  Pf6N2E2_5547 0.17  -0.9 6,361,752 +  Pf6N2E2_5547 0.17  -0.1 6,361,752 +  Pf6N2E2_5547 0.17  -1.0 6,361,752 +  Pf6N2E2_5547 0.17  -0.5 6,361,752 +  Pf6N2E2_5547 0.17  +0.0 6,361,753 -  Pf6N2E2_5547 0.17  -0.2 6,361,753 -  Pf6N2E2_5547 0.17  +1.7 6,361,753 -  Pf6N2E2_5547 0.17  -1.1 6,361,753 -  Pf6N2E2_5547 0.17  +1.5 6,361,753 -  Pf6N2E2_5547 0.17  -0.7 6,361,753 -  Pf6N2E2_5547 0.17  -0.5 6,361,769 -  Pf6N2E2_5547 0.19  +0.5 6,361,769 -  Pf6N2E2_5547 0.19  +0.2 6,361,851 +  Pf6N2E2_5547 0.24  -0.9 6,361,851 +  Pf6N2E2_5547 0.24  -0.3 6,361,851 +  Pf6N2E2_5547 0.24  -0.4 6,361,851 +  Pf6N2E2_5547 0.24  +0.2 6,361,852 -  Pf6N2E2_5547 0.24  -0.3 6,361,852 -  Pf6N2E2_5547 0.24  +1.2 6,361,918 +  Pf6N2E2_5547 0.29  -1.2 6,361,919 -  Pf6N2E2_5547 0.29  -0.0 6,361,950 +  Pf6N2E2_5547 0.31  +0.0 6,361,951 -  Pf6N2E2_5547 0.31  -0.9 6,361,951 -  Pf6N2E2_5547 0.31  -1.0 6,361,951 -  Pf6N2E2_5547 0.31  +0.7 6,362,008 +  Pf6N2E2_5547 0.35  -1.5 6,362,008 +  Pf6N2E2_5547 0.35  -0.3 6,362,009 -  Pf6N2E2_5547 0.35  -1.3 6,362,009 -  Pf6N2E2_5547 0.35  +0.5 6,362,009 -  Pf6N2E2_5547 0.35  -0.5 6,362,009 -  Pf6N2E2_5547 0.35  -1.1 6,362,009 -  Pf6N2E2_5547 0.35  +1.5 6,362,034 +  Pf6N2E2_5547 0.37  -0.4 6,362,034 +  Pf6N2E2_5547 0.37  +0.1 6,362,035 -  Pf6N2E2_5547 0.37  -0.3 6,362,035 -  Pf6N2E2_5547 0.37  -0.4 6,362,035 -  Pf6N2E2_5547 0.37  +0.7 6,362,089 +  Pf6N2E2_5547 0.41  +0.5 6,362,089 +  Pf6N2E2_5547 0.41  -1.3 6,362,090 -  Pf6N2E2_5547 0.41  -0.3 6,362,090 -  Pf6N2E2_5547 0.41  +0.0 6,362,122 +  Pf6N2E2_5547 0.43  -2.1 6,362,122 +  Pf6N2E2_5547 0.43  -2.0 6,362,122 +  Pf6N2E2_5547 0.43  -0.7 6,362,122 +  Pf6N2E2_5547 0.43  -0.1 6,362,122 +  Pf6N2E2_5547 0.43  -0.1 6,362,122 +  Pf6N2E2_5547 0.43  +1.2 6,362,123 -  Pf6N2E2_5547 0.43  +0.4 6,362,123 -  Pf6N2E2_5547 0.43  +1.0 6,362,123 -  Pf6N2E2_5547 0.43  -0.1 6,362,123 -  Pf6N2E2_5547 0.43  +0.6 6,362,123 -  Pf6N2E2_5547 0.43  +1.2 6,362,123 -  Pf6N2E2_5547 0.43  -0.2 6,362,123 -  Pf6N2E2_5547 0.43  -1.0 6,362,123 -  Pf6N2E2_5547 0.43  -1.0 6,362,130 +  Pf6N2E2_5547 0.44  -0.7 6,362,130 +  Pf6N2E2_5547 0.44  -0.4 6,362,130 +  Pf6N2E2_5547 0.44  +1.5 6,362,130 +  Pf6N2E2_5547 0.44  -0.3 6,362,131 -  Pf6N2E2_5547 0.44  +0.4 6,362,131 -  Pf6N2E2_5547 0.44  -0.7 6,362,131 -  Pf6N2E2_5547 0.44  -1.0 6,362,175 +  Pf6N2E2_5547 0.47  +1.0 
Or see this region's nucleotide sequence