Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4536

Experiment: Hydrochloric Acid 0.005 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4535 and Pf6N2E2_4536 overlap by 4 nucleotidesPf6N2E2_4536 and Pf6N2E2_4537 are separated by 9 nucleotidesPf6N2E2_4537 and Pf6N2E2_4538 overlap by 1 nucleotides Pf6N2E2_4535 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 5,269,316 to 5,270,419 _4535 Pf6N2E2_4536 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 5,270,416 to 5,271,069 _4536 Pf6N2E2_4537 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 5,271,079 to 5,271,333 _4537 Pf6N2E2_4538 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 5,271,333 to 5,272,130 _4538 Position (kb) 5270 5271 5272Strain fitness (log2 ratio) -2 -1 0 1at 5269.501 kb on + strand, within Pf6N2E2_4535at 5269.502 kb on - strand, within Pf6N2E2_4535at 5269.502 kb on - strand, within Pf6N2E2_4535at 5269.502 kb on - strand, within Pf6N2E2_4535at 5269.502 kb on - strand, within Pf6N2E2_4535at 5269.645 kb on + strand, within Pf6N2E2_4535at 5269.646 kb on - strand, within Pf6N2E2_4535at 5270.136 kb on - strand, within Pf6N2E2_4535at 5270.330 kb on + strandat 5270.330 kb on + strandat 5270.331 kb on - strandat 5270.494 kb on - strand, within Pf6N2E2_4536at 5270.517 kb on - strand, within Pf6N2E2_4536at 5270.584 kb on + strand, within Pf6N2E2_4536at 5270.585 kb on - strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.902 kb on + strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5270.903 kb on - strand, within Pf6N2E2_4536at 5271.055 kb on + strandat 5271.055 kb on + strandat 5271.055 kb on + strandat 5271.055 kb on + strandat 5271.056 kb on - strandat 5271.056 kb on - strandat 5271.056 kb on - strandat 5271.056 kb on - strandat 5271.056 kb on - strandat 5271.061 kb on + strandat 5271.061 kb on + strandat 5271.061 kb on + strandat 5271.100 kb on - strandat 5271.167 kb on + strand, within Pf6N2E2_4537at 5271.167 kb on + strand, within Pf6N2E2_4537at 5271.328 kb on - strandat 5271.357 kb on + strandat 5271.403 kb on - strandat 5271.414 kb on + strand, within Pf6N2E2_4538at 5271.621 kb on + strand, within Pf6N2E2_4538at 5271.621 kb on + strand, within Pf6N2E2_4538at 5271.621 kb on + strand, within Pf6N2E2_4538at 5271.622 kb on - strand, within Pf6N2E2_4538at 5271.622 kb on - strand, within Pf6N2E2_4538at 5271.798 kb on + strand, within Pf6N2E2_4538at 5271.798 kb on + strand, within Pf6N2E2_4538at 5271.798 kb on + strand, within Pf6N2E2_4538at 5271.798 kb on + strand, within Pf6N2E2_4538at 5271.798 kb on + strand, within Pf6N2E2_4538at 5271.964 kb on - strand, within Pf6N2E2_4538at 5271.964 kb on - strand, within Pf6N2E2_4538at 5271.964 kb on - strand, within Pf6N2E2_4538at 5271.965 kb on + strand, within Pf6N2E2_4538at 5272.039 kb on - strand, within Pf6N2E2_4538

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydrochloric Acid 0.005 M
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5,269,501 + Pf6N2E2_4535 0.17 -0.1
5,269,502 - Pf6N2E2_4535 0.17 -1.6
5,269,502 - Pf6N2E2_4535 0.17 -0.7
5,269,502 - Pf6N2E2_4535 0.17 -0.2
5,269,502 - Pf6N2E2_4535 0.17 +0.0
5,269,645 + Pf6N2E2_4535 0.30 +0.7
5,269,646 - Pf6N2E2_4535 0.30 -0.3
5,270,136 - Pf6N2E2_4535 0.74 -0.1
5,270,330 + -1.1
5,270,330 + +0.1
5,270,331 - -0.3
5,270,494 - Pf6N2E2_4536 0.12 +0.2
5,270,517 - Pf6N2E2_4536 0.15 +0.1
5,270,584 + Pf6N2E2_4536 0.26 +0.1
5,270,585 - Pf6N2E2_4536 0.26 -1.0
5,270,902 + Pf6N2E2_4536 0.74 -2.1
5,270,902 + Pf6N2E2_4536 0.74 +0.0
5,270,902 + Pf6N2E2_4536 0.74 +1.0
5,270,902 + Pf6N2E2_4536 0.74 +0.0
5,270,902 + Pf6N2E2_4536 0.74 +0.1
5,270,902 + Pf6N2E2_4536 0.74 +0.0
5,270,902 + Pf6N2E2_4536 0.74 -0.2
5,270,902 + Pf6N2E2_4536 0.74 -1.3
5,270,903 - Pf6N2E2_4536 0.74 +0.1
5,270,903 - Pf6N2E2_4536 0.74 -1.1
5,270,903 - Pf6N2E2_4536 0.74 +0.3
5,270,903 - Pf6N2E2_4536 0.74 -0.4
5,270,903 - Pf6N2E2_4536 0.74 -0.3
5,270,903 - Pf6N2E2_4536 0.74 -1.5
5,270,903 - Pf6N2E2_4536 0.74 -0.6
5,271,055 + +0.9
5,271,055 + +0.8
5,271,055 + +0.1
5,271,055 + -1.0
5,271,056 - +0.1
5,271,056 - -0.2
5,271,056 - +0.9
5,271,056 - +0.5
5,271,056 - +0.2
5,271,061 + +0.2
5,271,061 + +0.2
5,271,061 + -0.1
5,271,100 - -0.1
5,271,167 + Pf6N2E2_4537 0.35 +0.0
5,271,167 + Pf6N2E2_4537 0.35 -0.1
5,271,328 - +0.1
5,271,357 + -1.4
5,271,403 - +0.9
5,271,414 + Pf6N2E2_4538 0.10 -0.4
5,271,621 + Pf6N2E2_4538 0.36 +0.3
5,271,621 + Pf6N2E2_4538 0.36 +0.6
5,271,621 + Pf6N2E2_4538 0.36 +0.4
5,271,622 - Pf6N2E2_4538 0.36 -0.5
5,271,622 - Pf6N2E2_4538 0.36 +0.2
5,271,798 + Pf6N2E2_4538 0.58 +0.7
5,271,798 + Pf6N2E2_4538 0.58 -0.4
5,271,798 + Pf6N2E2_4538 0.58 -1.1
5,271,798 + Pf6N2E2_4538 0.58 +1.1
5,271,798 + Pf6N2E2_4538 0.58 +1.3
5,271,964 - Pf6N2E2_4538 0.79 +0.4
5,271,964 - Pf6N2E2_4538 0.79 -1.2
5,271,964 - Pf6N2E2_4538 0.79 +0.9
5,271,965 + Pf6N2E2_4538 0.79 +0.3
5,272,039 - Pf6N2E2_4538 0.88 +0.5

Or see this region's nucleotide sequence