Experiment: m.b. Sisomicin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4084 and Dshi_4085 overlap by 14 nucleotides Dshi_4085 and Dshi_4086 are separated by 143 nucleotides Dshi_4086 and Dshi_4087 overlap by 14 nucleotides Dshi_4087 and Dshi_4088 are separated by 5 nucleotides Dshi_4088 and Dshi_4089 are separated by 161 nucleotides
Dshi_4084: Dshi_4084 - Resolvase domain (RefSeq), at 19,415 to 19,981
_4084
Dshi_4085: Dshi_4085 - WGR domain protein (RefSeq), at 19,968 to 20,222
_4085
Dshi_4086: Dshi_4086 - hypothetical protein (RefSeq), at 20,366 to 20,572
_4086
Dshi_4087: Dshi_4087 - plasmid stabilization system (RefSeq), at 20,559 to 20,852
_4087
Dshi_4088: Dshi_4088 - hypothetical protein (RefSeq), at 20,858 to 21,250
_4088
Dshi_4089: Dshi_4089 - Integrase catalytic region (RefSeq), at 21,412 to 22,221
_4089
Position (kb) 20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 19.419 kb on + strand at 19.420 kb on + strand at 19.420 kb on + strand at 19.426 kb on - strand at 19.427 kb on - strand at 19.428 kb on - strand at 19.453 kb on + strand at 19.453 kb on + strand at 19.461 kb on - strand at 19.461 kb on - strand at 19.461 kb on - strand at 19.466 kb on - strand at 19.476 kb on - strand, within Dshi_4084 at 19.476 kb on - strand, within Dshi_4084 at 19.491 kb on + strand, within Dshi_4084 at 19.593 kb on + strand, within Dshi_4084 at 19.620 kb on - strand, within Dshi_4084 at 19.728 kb on + strand, within Dshi_4084 at 19.751 kb on - strand, within Dshi_4084 at 19.751 kb on - strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.796 kb on - strand, within Dshi_4084 at 19.796 kb on - strand, within Dshi_4084 at 19.798 kb on - strand, within Dshi_4084 at 19.798 kb on - strand, within Dshi_4084 at 19.833 kb on + strand, within Dshi_4084 at 19.841 kb on - strand, within Dshi_4084 at 19.875 kb on + strand, within Dshi_4084 at 19.885 kb on + strand, within Dshi_4084 at 19.894 kb on - strand, within Dshi_4084 at 19.900 kb on + strand, within Dshi_4084 at 19.900 kb on + strand, within Dshi_4084 at 19.908 kb on - strand, within Dshi_4084 at 19.908 kb on - strand, within Dshi_4084 at 19.961 kb on - strand at 20.012 kb on + strand, within Dshi_4085 at 20.012 kb on + strand, within Dshi_4085 at 20.069 kb on + strand, within Dshi_4085 at 20.069 kb on + strand, within Dshi_4085 at 20.077 kb on - strand, within Dshi_4085 at 20.080 kb on + strand, within Dshi_4085 at 20.121 kb on - strand, within Dshi_4085 at 20.121 kb on - strand, within Dshi_4085 at 20.136 kb on - strand, within Dshi_4085 at 20.145 kb on + strand, within Dshi_4085 at 20.153 kb on - strand, within Dshi_4085 at 20.160 kb on + strand, within Dshi_4085 at 20.180 kb on + strand, within Dshi_4085 at 20.193 kb on - strand, within Dshi_4085 at 20.203 kb on - strand at 20.213 kb on + strand at 20.218 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.228 kb on + strand at 20.228 kb on + strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.233 kb on + strand at 20.236 kb on - strand at 20.236 kb on - strand at 20.236 kb on - strand at 20.241 kb on - strand at 20.253 kb on - strand at 20.300 kb on - strand at 20.302 kb on + strand at 20.551 kb on + strand, within Dshi_4086 at 20.566 kb on + strand at 20.566 kb on + strand at 20.566 kb on + strand at 20.574 kb on - strand at 20.585 kb on + strand at 20.593 kb on - strand, within Dshi_4087 at 20.600 kb on + strand, within Dshi_4087 at 20.640 kb on + strand, within Dshi_4087 at 20.640 kb on + strand, within Dshi_4087 at 20.640 kb on + strand, within Dshi_4087 at 20.677 kb on + strand, within Dshi_4087 at 20.677 kb on + strand, within Dshi_4087 at 20.677 kb on + strand, within Dshi_4087 at 20.678 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on - strand, within Dshi_4087 at 20.685 kb on - strand, within Dshi_4087 at 20.696 kb on - strand, within Dshi_4087 at 20.698 kb on + strand, within Dshi_4087 at 20.698 kb on + strand, within Dshi_4087 at 20.699 kb on - strand, within Dshi_4087 at 20.699 kb on - strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.706 kb on - strand, within Dshi_4087 at 20.716 kb on - strand, within Dshi_4087 at 20.718 kb on - strand, within Dshi_4087 at 20.718 kb on - strand, within Dshi_4087 at 20.718 kb on - strand, within Dshi_4087 at 20.736 kb on + strand, within Dshi_4087 at 20.736 kb on + strand, within Dshi_4087 at 20.736 kb on + strand, within Dshi_4087 at 20.747 kb on - strand, within Dshi_4087 at 20.762 kb on + strand, within Dshi_4087 at 20.771 kb on + strand, within Dshi_4087 at 20.793 kb on + strand, within Dshi_4087 at 20.801 kb on + strand, within Dshi_4087 at 20.816 kb on - strand, within Dshi_4087 at 20.833 kb on - strand at 20.870 kb on - strand at 20.871 kb on + strand at 20.905 kb on - strand, within Dshi_4088 at 20.945 kb on - strand, within Dshi_4088 at 20.946 kb on + strand, within Dshi_4088 at 20.949 kb on - strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.952 kb on + strand, within Dshi_4088 at 20.961 kb on + strand, within Dshi_4088 at 21.000 kb on - strand, within Dshi_4088 at 21.017 kb on + strand, within Dshi_4088 at 21.017 kb on + strand, within Dshi_4088 at 21.024 kb on - strand, within Dshi_4088 at 21.026 kb on + strand, within Dshi_4088 at 21.026 kb on + strand, within Dshi_4088 at 21.047 kb on + strand, within Dshi_4088 at 21.057 kb on + strand, within Dshi_4088 at 21.059 kb on - strand, within Dshi_4088 at 21.065 kb on - strand, within Dshi_4088 at 21.129 kb on - strand, within Dshi_4088 at 21.150 kb on + strand, within Dshi_4088 at 21.152 kb on + strand, within Dshi_4088 at 21.165 kb on + strand, within Dshi_4088 at 21.165 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.203 kb on - strand, within Dshi_4088 at 21.228 kb on + strand at 21.234 kb on + strand at 21.240 kb on + strand at 21.243 kb on - strand at 21.255 kb on + strand at 21.255 kb on + strand at 21.263 kb on - strand at 21.330 kb on + strand at 21.344 kb on + strand at 21.349 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.002 mg/ml remove 19,419 + +0.9 19,420 + -0.5 19,420 + +0.2 19,426 - +1.6 19,427 - -0.1 19,428 - -0.3 19,453 + +0.9 19,453 + +0.5 19,461 - +0.9 19,461 - -1.1 19,461 - +0.9 19,466 - -0.0 19,476 - Dshi_4084 0.11 +2.2 19,476 - Dshi_4084 0.11 +0.5 19,491 + Dshi_4084 0.13 +0.4 19,593 + Dshi_4084 0.31 +0.1 19,620 - Dshi_4084 0.36 +0.4 19,728 + Dshi_4084 0.55 +1.2 19,751 - Dshi_4084 0.59 -0.2 19,751 - Dshi_4084 0.59 +0.9 19,790 + Dshi_4084 0.66 -0.7 19,790 + Dshi_4084 0.66 -0.0 19,790 + Dshi_4084 0.66 -0.0 19,796 - Dshi_4084 0.67 +0.6 19,796 - Dshi_4084 0.67 -2.6 19,798 - Dshi_4084 0.68 +0.6 19,798 - Dshi_4084 0.68 +0.9 19,833 + Dshi_4084 0.74 +0.2 19,841 - Dshi_4084 0.75 -0.2 19,875 + Dshi_4084 0.81 -0.3 19,885 + Dshi_4084 0.83 +0.0 19,894 - Dshi_4084 0.84 -0.5 19,900 + Dshi_4084 0.86 -0.1 19,900 + Dshi_4084 0.86 +0.4 19,908 - Dshi_4084 0.87 +1.6 19,908 - Dshi_4084 0.87 -0.5 19,961 - +1.6 20,012 + Dshi_4085 0.17 -1.3 20,012 + Dshi_4085 0.17 -0.1 20,069 + Dshi_4085 0.40 -1.6 20,069 + Dshi_4085 0.40 -0.6 20,077 - Dshi_4085 0.43 -0.3 20,080 + Dshi_4085 0.44 +1.3 20,121 - Dshi_4085 0.60 -0.1 20,121 - Dshi_4085 0.60 +0.2 20,136 - Dshi_4085 0.66 -1.1 20,145 + Dshi_4085 0.69 -1.4 20,153 - Dshi_4085 0.73 +0.5 20,160 + Dshi_4085 0.75 -0.7 20,180 + Dshi_4085 0.83 -0.9 20,193 - Dshi_4085 0.88 -0.4 20,203 - -1.0 20,213 + -0.9 20,218 + -0.0 20,223 + +1.1 20,223 + +0.4 20,223 + +0.3 20,223 + -0.3 20,223 + -0.1 20,223 + -1.3 20,223 + -0.9 20,223 + -0.7 20,223 + +0.4 20,223 + +1.9 20,228 + -0.4 20,228 + -0.4 20,231 - -1.1 20,231 - +0.1 20,231 - +1.3 20,231 - -1.4 20,231 - +1.7 20,231 - +1.2 20,231 - +2.5 20,231 - -0.3 20,231 - -2.1 20,231 - -0.4 20,231 - +0.2 20,231 - +0.1 20,231 - -0.8 20,233 + +0.1 20,236 - -1.1 20,236 - +1.4 20,236 - -0.3 20,241 - -1.7 20,253 - +1.6 20,300 - +0.6 20,302 + -1.7 20,551 + Dshi_4086 0.89 -0.1 20,566 + +0.6 20,566 + +0.5 20,566 + +0.1 20,574 - +0.9 20,585 + -1.1 20,593 - Dshi_4087 0.12 -0.7 20,600 + Dshi_4087 0.14 +0.3 20,640 + Dshi_4087 0.28 -0.3 20,640 + Dshi_4087 0.28 -0.3 20,640 + Dshi_4087 0.28 +0.7 20,677 + Dshi_4087 0.40 +0.3 20,677 + Dshi_4087 0.40 -1.1 20,677 + Dshi_4087 0.40 -0.2 20,678 + Dshi_4087 0.40 -0.6 20,685 + Dshi_4087 0.43 +0.1 20,685 + Dshi_4087 0.43 +0.3 20,685 + Dshi_4087 0.43 +0.1 20,685 + Dshi_4087 0.43 -1.4 20,685 - Dshi_4087 0.43 -0.3 20,685 - Dshi_4087 0.43 -0.5 20,696 - Dshi_4087 0.47 -1.1 20,698 + Dshi_4087 0.47 +0.6 20,698 + Dshi_4087 0.47 -1.1 20,699 - Dshi_4087 0.48 +0.4 20,699 - Dshi_4087 0.48 +0.9 20,700 + Dshi_4087 0.48 -0.3 20,700 + Dshi_4087 0.48 -0.1 20,700 + Dshi_4087 0.48 +1.9 20,700 + Dshi_4087 0.48 +0.9 20,700 + Dshi_4087 0.48 -0.1 20,700 + Dshi_4087 0.48 +0.2 20,706 - Dshi_4087 0.50 +0.4 20,716 - Dshi_4087 0.53 -1.0 20,718 - Dshi_4087 0.54 -0.3 20,718 - Dshi_4087 0.54 -0.3 20,718 - Dshi_4087 0.54 +0.1 20,736 + Dshi_4087 0.60 -0.1 20,736 + Dshi_4087 0.60 -0.6 20,736 + Dshi_4087 0.60 -0.6 20,747 - Dshi_4087 0.64 +1.9 20,762 + Dshi_4087 0.69 +0.6 20,771 + Dshi_4087 0.72 +0.9 20,793 + Dshi_4087 0.80 +1.0 20,801 + Dshi_4087 0.82 +0.9 20,816 - Dshi_4087 0.87 +0.0 20,833 - +1.5 20,870 - +0.1 20,871 + -2.8 20,905 - Dshi_4088 0.12 -1.7 20,945 - Dshi_4088 0.22 -1.2 20,946 + Dshi_4088 0.22 -0.2 20,949 - Dshi_4088 0.23 +0.3 20,951 + Dshi_4088 0.24 +0.4 20,951 + Dshi_4088 0.24 +0.3 20,951 + Dshi_4088 0.24 -0.5 20,951 + Dshi_4088 0.24 +1.5 20,951 + Dshi_4088 0.24 -1.3 20,951 + Dshi_4088 0.24 +0.6 20,952 + Dshi_4088 0.24 -0.6 20,961 + Dshi_4088 0.26 -0.5 21,000 - Dshi_4088 0.36 -0.7 21,017 + Dshi_4088 0.40 -0.2 21,017 + Dshi_4088 0.40 +0.5 21,024 - Dshi_4088 0.42 +0.2 21,026 + Dshi_4088 0.43 -0.8 21,026 + Dshi_4088 0.43 -0.7 21,047 + Dshi_4088 0.48 -0.8 21,057 + Dshi_4088 0.51 +1.1 21,059 - Dshi_4088 0.51 -1.0 21,065 - Dshi_4088 0.53 -0.7 21,129 - Dshi_4088 0.69 -0.9 21,150 + Dshi_4088 0.74 +0.1 21,152 + Dshi_4088 0.75 +0.7 21,165 + Dshi_4088 0.78 -0.8 21,165 - Dshi_4088 0.78 -0.5 21,173 - Dshi_4088 0.80 -0.7 21,173 - Dshi_4088 0.80 -0.5 21,173 - Dshi_4088 0.80 -0.1 21,173 - Dshi_4088 0.80 -0.3 21,173 - Dshi_4088 0.80 +0.1 21,173 - Dshi_4088 0.80 +0.5 21,173 - Dshi_4088 0.80 +0.3 21,203 - Dshi_4088 0.88 -0.1 21,228 + -0.0 21,234 + +0.3 21,240 + +0.4 21,243 - +0.4 21,255 + -0.1 21,255 + +0.9 21,263 - -0.6 21,330 + -0.2 21,344 + +0.1 21,349 + +0.1
Or see this region's nucleotide sequence