Experiment: m.b. Sisomicin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2711 and Dshi_2712 are separated by 60 nucleotides Dshi_2712 and Dshi_2713 are separated by 16 nucleotides Dshi_2713 and Dshi_2714 overlap by 4 nucleotides
Dshi_2711: Dshi_2711 - cobalt chelatase, pCobS small subunit (RefSeq), at 2,873,040 to 2,874,023
_2711
Dshi_2712: Dshi_2712 - Phosphatidylethanolamine N-methyltransferase (RefSeq), at 2,874,084 to 2,874,764
_2712
Dshi_2713: Dshi_2713 - CDP-diacylglycerol--serineO-phosphatidyltransferase (RefSeq), at 2,874,781 to 2,875,530
_2713
Dshi_2714: Dshi_2714 - phosphatidylserine decarboxylase related protein (RefSeq), at 2,875,527 to 2,876,204
_2714
Position (kb)
2874
2875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2874.070 kb on - strand at 2874.216 kb on + strand, within Dshi_2712 at 2874.427 kb on + strand, within Dshi_2712 at 2874.432 kb on + strand, within Dshi_2712 at 2874.432 kb on + strand, within Dshi_2712 at 2874.526 kb on + strand, within Dshi_2712 at 2874.534 kb on - strand, within Dshi_2712 at 2874.630 kb on + strand, within Dshi_2712 at 2874.722 kb on - strand at 2874.742 kb on - strand at 2874.794 kb on - strand at 2874.836 kb on - strand at 2874.850 kb on - strand at 2875.009 kb on - strand, within Dshi_2713 at 2875.044 kb on - strand, within Dshi_2713 at 2875.078 kb on - strand, within Dshi_2713 at 2875.157 kb on + strand, within Dshi_2713 at 2875.246 kb on + strand, within Dshi_2713 at 2875.363 kb on + strand, within Dshi_2713 at 2875.433 kb on + strand, within Dshi_2713 at 2875.435 kb on + strand, within Dshi_2713 at 2875.461 kb on + strand at 2875.623 kb on - strand, within Dshi_2714
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.002 mg/ml remove 2,874,070 - -0.3 2,874,216 + Dshi_2712 0.19 +0.3 2,874,427 + Dshi_2712 0.50 +0.0 2,874,432 + Dshi_2712 0.51 -1.0 2,874,432 + Dshi_2712 0.51 -0.5 2,874,526 + Dshi_2712 0.65 -0.6 2,874,534 - Dshi_2712 0.66 -1.9 2,874,630 + Dshi_2712 0.80 -1.3 2,874,722 - -0.2 2,874,742 - -1.0 2,874,794 - +0.6 2,874,836 - -0.1 2,874,850 - -3.4 2,875,009 - Dshi_2713 0.30 -1.6 2,875,044 - Dshi_2713 0.35 -2.1 2,875,078 - Dshi_2713 0.40 -3.0 2,875,157 + Dshi_2713 0.50 -2.8 2,875,246 + Dshi_2713 0.62 -3.1 2,875,363 + Dshi_2713 0.78 -4.5 2,875,433 + Dshi_2713 0.87 -0.5 2,875,435 + Dshi_2713 0.87 -1.9 2,875,461 + -2.9 2,875,623 - Dshi_2714 0.14 -2.5
Or see this region's nucleotide sequence