Experiment: LB_Aerobic_180MIN_ZN14B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_01620 and CSW01_01625 are separated by 94 nucleotides CSW01_01625 and CSW01_01630 are separated by 12 nucleotides CSW01_01630 and CSW01_01635 overlap by 4 nucleotides
CSW01_01620: CSW01_01620 - phosphatidylserine synthase, at 328,451 to 329,791
_01620
CSW01_01625: CSW01_01625 - N-acetyltransferase, at 329,886 to 330,332
_01625
CSW01_01630: CSW01_01630 - UDP-N-acetylmuramate dehydrogenase, at 330,345 to 331,418
_01630
CSW01_01635: CSW01_01635 - bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA, at 331,415 to 332,377
_01635
Position (kb) 330
331
332 Strain fitness (log2 ratio) -1
0
1 at 329.811 kb on - strand at 329.916 kb on + strand at 329.935 kb on - strand, within CSW01_01625 at 329.938 kb on + strand, within CSW01_01625 at 330.024 kb on + strand, within CSW01_01625 at 330.032 kb on - strand, within CSW01_01625 at 330.055 kb on + strand, within CSW01_01625 at 330.108 kb on + strand, within CSW01_01625 at 330.108 kb on + strand, within CSW01_01625 at 330.116 kb on - strand, within CSW01_01625 at 330.116 kb on - strand, within CSW01_01625 at 330.130 kb on - strand, within CSW01_01625 at 330.175 kb on - strand, within CSW01_01625 at 330.210 kb on - strand, within CSW01_01625 at 330.246 kb on - strand, within CSW01_01625 at 330.246 kb on - strand, within CSW01_01625 at 331.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Aerobic_180MIN_ZN14B remove 329,811 - -0.6 329,916 + -0.0 329,935 - CSW01_01625 0.11 +0.7 329,938 + CSW01_01625 0.12 -0.0 330,024 + CSW01_01625 0.31 -1.0 330,032 - CSW01_01625 0.33 +0.8 330,055 + CSW01_01625 0.38 +0.2 330,108 + CSW01_01625 0.50 -0.6 330,108 + CSW01_01625 0.50 +0.8 330,116 - CSW01_01625 0.51 +1.0 330,116 - CSW01_01625 0.51 +0.8 330,130 - CSW01_01625 0.55 -0.2 330,175 - CSW01_01625 0.65 -0.2 330,210 - CSW01_01625 0.72 -1.5 330,246 - CSW01_01625 0.81 +1.0 330,246 - CSW01_01625 0.81 -1.2 331,423 + -0.3
Or see this region's nucleotide sequence