Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS14205

Experiment: Temperature shift 30_to_4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_4
3,176,391	+		PFLU_RS14200	0.23	-0.1
3,176,391	+		PFLU_RS14200	0.23	+1.8
3,176,391	+		PFLU_RS14200	0.23	+1.5
3,176,391	+		PFLU_RS14200	0.23	+0.7
3,176,391	+		PFLU_RS14200	0.23	+0.7
3,176,391	+		PFLU_RS14200	0.23	-1.7
3,176,391	+		PFLU_RS14200	0.23	+0.5
3,176,392	-		PFLU_RS14200	0.23	+0.1
3,176,392	-		PFLU_RS14200	0.23	+0.4
3,176,392	-		PFLU_RS14200	0.23	-1.4
3,176,392	-		PFLU_RS14200	0.23	-0.6
3,176,462	+		PFLU_RS14200	0.29	-1.2
3,176,462	+		PFLU_RS14200	0.29	-0.3
3,176,462	+		PFLU_RS14200	0.29	+0.5
3,176,462	+		PFLU_RS14200	0.29	-0.4
3,176,462	+		PFLU_RS14200	0.29	-0.7
3,176,463	-		PFLU_RS14200	0.29	+3.1
3,176,463	-		PFLU_RS14200	0.29	+1.1
3,176,463	-		PFLU_RS14200	0.29	+0.8
3,176,463	-		PFLU_RS14200	0.29	+0.5
3,176,639	+		PFLU_RS14200	0.44	+0.9
3,176,640	-		PFLU_RS14200	0.44	+0.0
3,176,640	-		PFLU_RS14200	0.44	+0.3
3,176,723	+		PFLU_RS14200	0.52	+1.1
3,176,723	+		PFLU_RS14200	0.52	+1.2
3,176,724	-		PFLU_RS14200	0.52	+0.0
3,176,912	+		PFLU_RS14200	0.68	-1.0
3,176,912	+		PFLU_RS14200	0.68	-0.4
3,176,912	+		PFLU_RS14200	0.68	+0.5
3,176,912	+		PFLU_RS14200	0.68	+0.4
3,176,912	+		PFLU_RS14200	0.68	+1.2
3,176,912	+		PFLU_RS14200	0.68	+0.7
3,176,912	+		PFLU_RS14200	0.68	+0.9
3,176,913	-		PFLU_RS14200	0.68	-1.7
3,176,913	-		PFLU_RS14200	0.68	-0.9
3,176,913	-		PFLU_RS14200	0.68	-0.5
3,176,913	-		PFLU_RS14200	0.68	+0.1
3,176,913	-		PFLU_RS14200	0.68	-0.0
3,176,913	-		PFLU_RS14200	0.68	+0.1
3,176,913	-		PFLU_RS14200	0.68	-0.5
3,176,945	+		PFLU_RS14200	0.71	-0.4
3,176,945	+		PFLU_RS14200	0.71	-0.0
3,176,946	-		PFLU_RS14200	0.71	-0.5
3,176,946	-		PFLU_RS14200	0.71	-0.1
3,177,031	+		PFLU_RS14200	0.78	+0.5
3,177,040	+		PFLU_RS14200	0.79	+0.5
3,177,050	+		PFLU_RS14200	0.80	-0.9
3,177,050	+		PFLU_RS14200	0.80	+0.5
3,177,051	-		PFLU_RS14200	0.80	+0.5
3,177,051	-		PFLU_RS14200	0.80	-0.7
3,177,224	+				+0.1
3,177,224	+				+1.5
3,177,224	+				+0.3
3,177,224	+				+0.4
3,177,224	+				+0.1
3,177,224	+				-0.2
3,177,224	+				-1.2
3,177,224	+				+0.6
3,177,224	+				-0.5
3,177,224	+				+0.7
3,177,224	+				-1.7
3,177,224	+				+0.4
3,177,225	-				+0.1
3,177,225	-				-1.5
3,177,225	-				-0.3
3,177,225	-				-0.0
3,177,225	-				-0.5
3,177,225	-				+1.7
3,177,225	-				-0.5
3,177,225	-				-1.3
3,177,225	-				-1.3
3,177,225	-				+0.4
3,177,225	-				+0.1
3,177,258	-				+0.1
3,177,353	+				-1.3
3,177,353	+				-1.5
3,177,353	+				+1.7
3,177,354	-				-0.1
3,177,509	+		PFLU_RS14205	0.33	-0.9
3,177,509	+		PFLU_RS14205	0.33	+0.7
3,177,509	+		PFLU_RS14205	0.33	+0.5
3,177,510	-		PFLU_RS14205	0.33	+0.6
3,177,548	+		PFLU_RS14205	0.41	-0.5
3,177,548	+		PFLU_RS14205	0.41	-0.8
3,177,548	+		PFLU_RS14205	0.41	+0.4
3,177,548	+		PFLU_RS14205	0.41	-0.5
3,177,548	+		PFLU_RS14205	0.41	-1.2
3,177,549	-		PFLU_RS14205	0.41	+0.3
3,177,549	-		PFLU_RS14205	0.41	+0.9
3,177,554	+		PFLU_RS14205	0.42	-0.2
3,177,555	-		PFLU_RS14205	0.42	-0.5
3,177,665	+		PFLU_RS14205	0.65	+0.1
3,177,665	+		PFLU_RS14205	0.65	+1.8
3,177,707	+		PFLU_RS14205	0.74	+0.7
3,177,707	+		PFLU_RS14205	0.74	+1.1
3,177,708	-		PFLU_RS14205	0.74	+0.5
3,177,708	-		PFLU_RS14205	0.74	-0.3
3,177,708	-		PFLU_RS14205	0.74	+1.1
3,177,708	-		PFLU_RS14205	0.74	+0.1
3,177,852	+				+1.1
3,177,852	+				+2.3
3,177,853	-				+0.5
3,177,853	-				+0.9
3,178,052	-		PFLU_RS14210	0.29	+1.3
3,178,134	-		PFLU_RS14210	0.44	-0.7
3,178,387	+		PFLU_RS14210	0.88	+1.9
3,178,387	+		PFLU_RS14210	0.88	+1.1
3,178,387	+		PFLU_RS14210	0.88	+1.1
3,178,388	-		PFLU_RS14210	0.89	-1.2
3,178,442	+				+0.1
3,178,443	-				-1.2
3,178,443	-				+1.7
3,178,534	+				+0.5
3,178,534	+				+0.1
3,178,534	+				-0.5
3,178,534	+				-1.0
3,178,534	+				+0.6
3,178,535	-				+0.7
3,178,535	-				+1.6
3,178,535	-				+0.9
3,178,574	+				+1.9
3,178,574	+				+0.9
3,178,574	+				+3.9
3,178,575	-				+0.2
3,178,712	+		PFLU_RS14215	0.10	-1.3
3,178,712	+		PFLU_RS14215	0.10	-0.6
3,178,712	+		PFLU_RS14215	0.10	-0.6
3,178,712	+		PFLU_RS14215	0.10	-1.3
3,178,712	+		PFLU_RS14215	0.10	+1.3
3,178,712	+		PFLU_RS14215	0.10	-0.8
3,178,712	+		PFLU_RS14215	0.10	-0.2
3,178,712	+		PFLU_RS14215	0.10	+0.5
3,178,713	-		PFLU_RS14215	0.10	+1.7
3,178,716	+		PFLU_RS14215	0.10	-1.7
3,178,716	+		PFLU_RS14215	0.10	-0.9
3,178,716	+		PFLU_RS14215	0.10	+1.5
3,178,716	+		PFLU_RS14215	0.10	-0.5
3,178,716	+		PFLU_RS14215	0.10	+1.8
3,178,716	+		PFLU_RS14215	0.10	-0.5
3,178,716	+		PFLU_RS14215	0.10	-0.7
3,178,716	+		PFLU_RS14215	0.10	-1.2
3,178,716	+		PFLU_RS14215	0.10	-1.7
3,178,717	-		PFLU_RS14215	0.11	+0.9
3,178,717	-		PFLU_RS14215	0.11	-0.1
3,178,717	-		PFLU_RS14215	0.11	+0.7
3,178,717	-		PFLU_RS14215	0.11	-0.6
3,178,717	-		PFLU_RS14215	0.11	-0.2
3,178,717	-		PFLU_RS14215	0.11	-0.9
3,178,717	-		PFLU_RS14215	0.11	+0.1
3,178,717	-		PFLU_RS14215	0.11	-0.7
3,178,717	-		PFLU_RS14215	0.11	+1.1
3,178,717	-		PFLU_RS14215	0.11	-0.0
3,178,732	-		PFLU_RS14215	0.12	-0.1
3,178,764	+		PFLU_RS14215	0.14	+2.1
3,178,764	+		PFLU_RS14215	0.14	-0.6
3,178,764	+		PFLU_RS14215	0.14	-0.1
3,178,764	+		PFLU_RS14215	0.14	+0.1
3,178,764	+		PFLU_RS14215	0.14	+0.4
3,178,764	+		PFLU_RS14215	0.14	-0.2
3,178,765	-		PFLU_RS14215	0.14	+0.3
3,178,765	-		PFLU_RS14215	0.14	+0.6
3,178,765	-		PFLU_RS14215	0.14	+1.4
3,178,765	-		PFLU_RS14215	0.14	-1.3
3,178,765	-		PFLU_RS14215	0.14	-1.0
3,178,765	-		PFLU_RS14215	0.14	+0.8
3,178,765	-		PFLU_RS14215	0.14	+0.4
3,178,765	-		PFLU_RS14215	0.14	+0.1
3,178,765	-		PFLU_RS14215	0.14	+0.9