Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20435 and IAI47_20440 are separated by 225 nucleotides IAI47_20440 and IAI47_20445 are separated by 86 nucleotides
IAI47_20435: IAI47_20435 - fumarate hydratase, at 330,577 to 332,223
_20435
IAI47_20440: IAI47_20440 - SDR family oxidoreductase, at 332,449 to 333,105
_20440
IAI47_20445: IAI47_20445 - SDR family oxidoreductase, at 333,192 to 334,259
_20445
Position (kb) 332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 331.454 kb on - strand, within IAI47_20435 at 331.545 kb on + strand, within IAI47_20435 at 331.545 kb on + strand, within IAI47_20435 at 331.546 kb on - strand, within IAI47_20435 at 331.546 kb on - strand, within IAI47_20435 at 331.572 kb on + strand, within IAI47_20435 at 331.572 kb on + strand, within IAI47_20435 at 331.573 kb on - strand, within IAI47_20435 at 331.573 kb on - strand, within IAI47_20435 at 331.716 kb on + strand, within IAI47_20435 at 331.758 kb on + strand, within IAI47_20435 at 331.813 kb on + strand, within IAI47_20435 at 331.814 kb on - strand, within IAI47_20435 at 331.834 kb on + strand, within IAI47_20435 at 331.834 kb on + strand, within IAI47_20435 at 331.834 kb on + strand, within IAI47_20435 at 331.835 kb on - strand, within IAI47_20435 at 331.835 kb on - strand, within IAI47_20435 at 331.835 kb on - strand, within IAI47_20435 at 331.835 kb on - strand, within IAI47_20435 at 331.837 kb on + strand, within IAI47_20435 at 331.838 kb on - strand, within IAI47_20435 at 331.915 kb on + strand, within IAI47_20435 at 331.915 kb on + strand, within IAI47_20435 at 331.915 kb on + strand, within IAI47_20435 at 331.915 kb on + strand, within IAI47_20435 at 331.916 kb on - strand, within IAI47_20435 at 331.977 kb on + strand, within IAI47_20435 at 331.978 kb on - strand, within IAI47_20435 at 331.978 kb on - strand, within IAI47_20435 at 331.978 kb on - strand, within IAI47_20435 at 332.001 kb on + strand, within IAI47_20435 at 332.018 kb on - strand, within IAI47_20435 at 332.089 kb on + strand at 332.148 kb on + strand at 332.165 kb on + strand at 332.169 kb on + strand at 332.221 kb on + strand at 332.221 kb on + strand at 332.221 kb on + strand at 332.222 kb on - strand at 332.222 kb on - strand at 332.224 kb on + strand at 332.224 kb on + strand at 332.224 kb on + strand at 332.224 kb on + strand at 332.224 kb on + strand at 332.224 kb on + strand at 332.224 kb on + strand at 332.225 kb on - strand at 332.225 kb on - strand at 332.225 kb on - strand at 332.238 kb on - strand at 332.275 kb on - strand at 332.280 kb on + strand at 332.280 kb on + strand at 332.287 kb on + strand at 332.344 kb on - strand at 332.344 kb on - strand at 332.344 kb on - strand at 332.442 kb on + strand at 332.443 kb on - strand at 332.451 kb on - strand at 332.451 kb on - strand at 332.512 kb on + strand at 332.512 kb on + strand at 332.512 kb on + strand at 332.512 kb on + strand at 332.513 kb on - strand at 332.513 kb on - strand at 332.513 kb on - strand at 332.519 kb on + strand, within IAI47_20440 at 332.523 kb on + strand, within IAI47_20440 at 332.524 kb on - strand, within IAI47_20440 at 332.717 kb on + strand, within IAI47_20440 at 332.717 kb on + strand, within IAI47_20440 at 332.718 kb on - strand, within IAI47_20440 at 332.718 kb on - strand, within IAI47_20440 at 332.718 kb on - strand, within IAI47_20440 at 332.745 kb on + strand, within IAI47_20440 at 332.746 kb on - strand, within IAI47_20440 at 332.844 kb on + strand, within IAI47_20440 at 332.844 kb on + strand, within IAI47_20440 at 332.844 kb on + strand, within IAI47_20440 at 332.844 kb on + strand, within IAI47_20440 at 332.845 kb on - strand, within IAI47_20440 at 332.845 kb on - strand, within IAI47_20440 at 332.845 kb on - strand, within IAI47_20440 at 332.914 kb on + strand, within IAI47_20440 at 332.950 kb on - strand, within IAI47_20440 at 333.142 kb on + strand at 333.142 kb on + strand at 333.142 kb on + strand at 333.142 kb on + strand at 333.143 kb on - strand at 333.144 kb on + strand at 333.144 kb on + strand at 333.144 kb on + strand at 333.144 kb on + strand at 333.144 kb on + strand at 333.144 kb on + strand at 333.145 kb on - strand at 333.145 kb on - strand at 333.145 kb on - strand at 333.145 kb on - strand at 333.145 kb on - strand at 333.145 kb on - strand at 333.145 kb on - strand at 333.239 kb on + strand at 333.240 kb on - strand at 333.240 kb on - strand at 333.290 kb on + strand at 333.290 kb on + strand at 333.290 kb on + strand at 333.290 kb on + strand at 333.290 kb on + strand at 333.290 kb on + strand at 333.290 kb on + strand at 333.291 kb on - strand at 333.296 kb on + strand at 333.296 kb on + strand at 333.296 kb on + strand at 333.296 kb on + strand at 333.297 kb on - strand at 333.302 kb on + strand, within IAI47_20445 at 333.303 kb on - strand, within IAI47_20445 at 333.303 kb on - strand, within IAI47_20445 at 333.303 kb on - strand, within IAI47_20445 at 333.359 kb on + strand, within IAI47_20445 at 333.359 kb on + strand, within IAI47_20445 at 333.359 kb on + strand, within IAI47_20445 at 333.360 kb on - strand, within IAI47_20445 at 333.428 kb on + strand, within IAI47_20445 at 333.461 kb on - strand, within IAI47_20445 at 333.461 kb on - strand, within IAI47_20445 at 333.461 kb on - strand, within IAI47_20445 at 333.484 kb on + strand, within IAI47_20445 at 333.484 kb on + strand, within IAI47_20445 at 333.484 kb on + strand, within IAI47_20445 at 333.485 kb on - strand, within IAI47_20445 at 333.535 kb on - strand, within IAI47_20445 at 333.618 kb on - strand, within IAI47_20445 at 333.618 kb on - strand, within IAI47_20445 at 333.618 kb on - strand, within IAI47_20445 at 333.659 kb on + strand, within IAI47_20445 at 333.659 kb on + strand, within IAI47_20445 at 333.660 kb on - strand, within IAI47_20445 at 333.660 kb on - strand, within IAI47_20445 at 333.717 kb on - strand, within IAI47_20445 at 333.717 kb on - strand, within IAI47_20445 at 333.729 kb on - strand, within IAI47_20445 at 333.732 kb on + strand, within IAI47_20445 at 333.733 kb on - strand, within IAI47_20445 at 333.752 kb on + strand, within IAI47_20445 at 333.753 kb on - strand, within IAI47_20445 at 333.753 kb on - strand, within IAI47_20445 at 333.778 kb on + strand, within IAI47_20445 at 333.779 kb on - strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.784 kb on + strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.785 kb on - strand, within IAI47_20445 at 333.826 kb on + strand, within IAI47_20445 at 333.826 kb on + strand, within IAI47_20445 at 333.826 kb on + strand, within IAI47_20445 at 333.826 kb on + strand, within IAI47_20445 at 333.827 kb on - strand, within IAI47_20445 at 333.827 kb on - strand, within IAI47_20445 at 333.827 kb on - strand, within IAI47_20445 at 333.834 kb on - strand, within IAI47_20445 at 333.896 kb on - strand, within IAI47_20445 at 333.916 kb on + strand, within IAI47_20445 at 333.916 kb on + strand, within IAI47_20445 at 333.916 kb on + strand, within IAI47_20445 at 333.917 kb on - strand, within IAI47_20445 at 333.917 kb on - strand, within IAI47_20445 at 333.917 kb on - strand, within IAI47_20445 at 334.071 kb on + strand, within IAI47_20445 at 334.071 kb on + strand, within IAI47_20445 at 334.071 kb on + strand, within IAI47_20445 at 334.072 kb on - strand, within IAI47_20445
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 331,454 - IAI47_20435 0.53 +0.3 331,545 + IAI47_20435 0.59 -0.2 331,545 + IAI47_20435 0.59 -1.8 331,546 - IAI47_20435 0.59 +0.4 331,546 - IAI47_20435 0.59 -0.6 331,572 + IAI47_20435 0.60 -1.6 331,572 + IAI47_20435 0.60 -1.3 331,573 - IAI47_20435 0.60 +1.3 331,573 - IAI47_20435 0.60 +0.2 331,716 + IAI47_20435 0.69 -1.0 331,758 + IAI47_20435 0.72 +0.0 331,813 + IAI47_20435 0.75 +1.0 331,814 - IAI47_20435 0.75 -0.6 331,834 + IAI47_20435 0.76 -1.6 331,834 + IAI47_20435 0.76 -1.4 331,834 + IAI47_20435 0.76 +0.4 331,835 - IAI47_20435 0.76 -1.6 331,835 - IAI47_20435 0.76 +1.7 331,835 - IAI47_20435 0.76 -0.4 331,835 - IAI47_20435 0.76 -2.3 331,837 + IAI47_20435 0.77 +1.1 331,838 - IAI47_20435 0.77 +1.2 331,915 + IAI47_20435 0.81 -1.4 331,915 + IAI47_20435 0.81 +0.3 331,915 + IAI47_20435 0.81 +0.9 331,915 + IAI47_20435 0.81 -0.3 331,916 - IAI47_20435 0.81 +1.0 331,977 + IAI47_20435 0.85 -0.2 331,978 - IAI47_20435 0.85 +1.2 331,978 - IAI47_20435 0.85 -1.9 331,978 - IAI47_20435 0.85 -2.3 332,001 + IAI47_20435 0.86 +0.0 332,018 - IAI47_20435 0.87 +1.8 332,089 + -0.2 332,148 + +0.4 332,165 + +2.3 332,169 + +0.8 332,221 + -0.6 332,221 + -0.2 332,221 + +0.0 332,222 - -1.6 332,222 - +0.1 332,224 + -0.9 332,224 + -2.2 332,224 + -0.4 332,224 + -3.1 332,224 + +1.2 332,224 + -0.3 332,224 + +1.2 332,225 - +1.9 332,225 - -0.4 332,225 - +1.0 332,238 - +2.5 332,275 - -0.4 332,280 + +2.6 332,280 + +2.2 332,287 + +0.6 332,344 - -0.6 332,344 - +0.3 332,344 - -0.6 332,442 + +0.8 332,443 - +1.2 332,451 - +1.0 332,451 - -3.2 332,512 + -0.6 332,512 + +1.6 332,512 + -0.3 332,512 + -1.9 332,513 - +0.8 332,513 - -0.8 332,513 - -0.5 332,519 + IAI47_20440 0.11 -1.6 332,523 + IAI47_20440 0.11 -0.7 332,524 - IAI47_20440 0.11 -1.2 332,717 + IAI47_20440 0.41 +2.0 332,717 + IAI47_20440 0.41 +1.1 332,718 - IAI47_20440 0.41 +0.7 332,718 - IAI47_20440 0.41 -1.0 332,718 - IAI47_20440 0.41 -1.2 332,745 + IAI47_20440 0.45 +0.8 332,746 - IAI47_20440 0.45 +4.6 332,844 + IAI47_20440 0.60 -0.8 332,844 + IAI47_20440 0.60 +1.0 332,844 + IAI47_20440 0.60 -2.1 332,844 + IAI47_20440 0.60 -0.8 332,845 - IAI47_20440 0.60 -1.0 332,845 - IAI47_20440 0.60 -1.0 332,845 - IAI47_20440 0.60 -0.6 332,914 + IAI47_20440 0.71 -0.9 332,950 - IAI47_20440 0.76 -1.4 333,142 + +1.8 333,142 + -3.0 333,142 + -1.2 333,142 + -1.4 333,143 - +0.2 333,144 + -1.6 333,144 + -2.8 333,144 + +0.6 333,144 + +1.5 333,144 + +1.2 333,144 + -1.2 333,145 - +1.4 333,145 - -2.6 333,145 - +2.0 333,145 - +1.2 333,145 - -0.5 333,145 - -1.6 333,145 - -1.1 333,239 + -0.2 333,240 - -1.7 333,240 - -0.2 333,290 + -1.9 333,290 + +0.9 333,290 + -1.7 333,290 + +0.4 333,290 + +0.2 333,290 + +1.5 333,290 + +1.2 333,291 - +0.4 333,296 + +0.4 333,296 + -0.4 333,296 + +0.0 333,296 + +1.0 333,297 - +0.4 333,302 + IAI47_20445 0.10 +0.8 333,303 - IAI47_20445 0.10 +0.4 333,303 - IAI47_20445 0.10 -1.6 333,303 - IAI47_20445 0.10 -1.2 333,359 + IAI47_20445 0.16 +0.0 333,359 + IAI47_20445 0.16 -1.1 333,359 + IAI47_20445 0.16 -1.3 333,360 - IAI47_20445 0.16 -1.2 333,428 + IAI47_20445 0.22 -1.6 333,461 - IAI47_20445 0.25 -2.4 333,461 - IAI47_20445 0.25 +0.4 333,461 - IAI47_20445 0.25 -0.2 333,484 + IAI47_20445 0.27 -1.2 333,484 + IAI47_20445 0.27 +1.3 333,484 + IAI47_20445 0.27 -0.6 333,485 - IAI47_20445 0.27 +0.0 333,535 - IAI47_20445 0.32 +0.0 333,618 - IAI47_20445 0.40 -1.2 333,618 - IAI47_20445 0.40 +2.4 333,618 - IAI47_20445 0.40 -0.3 333,659 + IAI47_20445 0.44 -1.3 333,659 + IAI47_20445 0.44 -0.9 333,660 - IAI47_20445 0.44 -0.3 333,660 - IAI47_20445 0.44 +1.1 333,717 - IAI47_20445 0.49 -0.2 333,717 - IAI47_20445 0.49 -0.3 333,729 - IAI47_20445 0.50 +0.0 333,732 + IAI47_20445 0.51 +0.7 333,733 - IAI47_20445 0.51 -0.3 333,752 + IAI47_20445 0.52 -2.2 333,753 - IAI47_20445 0.53 +0.4 333,753 - IAI47_20445 0.53 +0.6 333,778 + IAI47_20445 0.55 +0.3 333,779 - IAI47_20445 0.55 -1.3 333,784 + IAI47_20445 0.55 +0.3 333,784 + IAI47_20445 0.55 +0.3 333,784 + IAI47_20445 0.55 +0.7 333,784 + IAI47_20445 0.55 +1.7 333,784 + IAI47_20445 0.55 +0.2 333,784 + IAI47_20445 0.55 +1.3 333,784 + IAI47_20445 0.55 +0.0 333,784 + IAI47_20445 0.55 -2.3 333,784 + IAI47_20445 0.55 -0.2 333,784 + IAI47_20445 0.55 +1.5 333,784 + IAI47_20445 0.55 +3.0 333,785 - IAI47_20445 0.56 +0.6 333,785 - IAI47_20445 0.56 -1.4 333,785 - IAI47_20445 0.56 +0.9 333,785 - IAI47_20445 0.56 -1.5 333,785 - IAI47_20445 0.56 +1.8 333,785 - IAI47_20445 0.56 +1.0 333,785 - IAI47_20445 0.56 +0.3 333,785 - IAI47_20445 0.56 +1.5 333,785 - IAI47_20445 0.56 -1.7 333,826 + IAI47_20445 0.59 -1.0 333,826 + IAI47_20445 0.59 +1.3 333,826 + IAI47_20445 0.59 +1.8 333,826 + IAI47_20445 0.59 +1.4 333,827 - IAI47_20445 0.59 +0.2 333,827 - IAI47_20445 0.59 -0.3 333,827 - IAI47_20445 0.59 +0.5 333,834 - IAI47_20445 0.60 -0.1 333,896 - IAI47_20445 0.66 +1.5 333,916 + IAI47_20445 0.68 -0.6 333,916 + IAI47_20445 0.68 -1.2 333,916 + IAI47_20445 0.68 +1.8 333,917 - IAI47_20445 0.68 +3.6 333,917 - IAI47_20445 0.68 +1.8 333,917 - IAI47_20445 0.68 +2.0 334,071 + IAI47_20445 0.82 -0.8 334,071 + IAI47_20445 0.82 +1.3 334,071 + IAI47_20445 0.82 -0.6 334,072 - IAI47_20445 0.82 -1.6
Or see this region's nucleotide sequence