Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_10130 and IAI47_10135 are separated by 10 nucleotides IAI47_10135 and IAI47_10140 overlap by 26 nucleotides IAI47_10140 and IAI47_10145 are separated by 14 nucleotides
IAI47_10130: IAI47_10130 - MFS transporter, at 2,144,301 to 2,145,605
_10130
IAI47_10135: IAI47_10135 - substrate-binding domain-containing protein, at 2,145,616 to 2,146,425
_10135
IAI47_10140: IAI47_10140 - LysR family transcriptional regulator, at 2,146,400 to 2,147,299
_10140
IAI47_10145: IAI47_10145 - winged helix-turn-helix transcriptional regulator, at 2,147,314 to 2,147,808
_10145
Position (kb)
2145
2146
2147 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2144.622 kb on + strand, within IAI47_10130 at 2144.623 kb on - strand, within IAI47_10130 at 2144.675 kb on - strand, within IAI47_10130 at 2144.718 kb on + strand, within IAI47_10130 at 2144.718 kb on + strand, within IAI47_10130 at 2144.719 kb on - strand, within IAI47_10130 at 2144.757 kb on + strand, within IAI47_10130 at 2144.757 kb on + strand, within IAI47_10130 at 2144.757 kb on + strand, within IAI47_10130 at 2144.758 kb on - strand, within IAI47_10130 at 2144.758 kb on - strand, within IAI47_10130 at 2144.796 kb on - strand, within IAI47_10130 at 2144.796 kb on - strand, within IAI47_10130 at 2144.796 kb on - strand, within IAI47_10130 at 2144.796 kb on - strand, within IAI47_10130 at 2144.820 kb on - strand, within IAI47_10130 at 2144.820 kb on - strand, within IAI47_10130 at 2144.871 kb on - strand, within IAI47_10130 at 2144.906 kb on + strand, within IAI47_10130 at 2144.906 kb on + strand, within IAI47_10130 at 2144.906 kb on + strand, within IAI47_10130 at 2144.907 kb on - strand, within IAI47_10130 at 2144.914 kb on + strand, within IAI47_10130 at 2144.914 kb on + strand, within IAI47_10130 at 2144.914 kb on + strand, within IAI47_10130 at 2144.915 kb on - strand, within IAI47_10130 at 2144.915 kb on - strand, within IAI47_10130 at 2144.915 kb on - strand, within IAI47_10130 at 2144.915 kb on - strand, within IAI47_10130 at 2144.915 kb on - strand, within IAI47_10130 at 2144.915 kb on - strand, within IAI47_10130 at 2145.002 kb on + strand, within IAI47_10130 at 2145.002 kb on + strand, within IAI47_10130 at 2145.003 kb on - strand, within IAI47_10130 at 2145.003 kb on - strand, within IAI47_10130 at 2145.075 kb on + strand, within IAI47_10130 at 2145.076 kb on - strand, within IAI47_10130 at 2145.145 kb on + strand, within IAI47_10130 at 2145.145 kb on + strand, within IAI47_10130 at 2145.146 kb on - strand, within IAI47_10130 at 2145.162 kb on - strand, within IAI47_10130 at 2145.206 kb on + strand, within IAI47_10130 at 2145.207 kb on - strand, within IAI47_10130 at 2145.214 kb on + strand, within IAI47_10130 at 2145.215 kb on - strand, within IAI47_10130 at 2145.215 kb on - strand, within IAI47_10130 at 2145.215 kb on - strand, within IAI47_10130 at 2145.252 kb on + strand, within IAI47_10130 at 2145.252 kb on + strand, within IAI47_10130 at 2145.252 kb on + strand, within IAI47_10130 at 2145.253 kb on - strand, within IAI47_10130 at 2145.253 kb on - strand, within IAI47_10130 at 2145.253 kb on - strand, within IAI47_10130 at 2145.339 kb on + strand, within IAI47_10130 at 2145.339 kb on + strand, within IAI47_10130 at 2145.374 kb on + strand, within IAI47_10130 at 2145.414 kb on + strand, within IAI47_10130 at 2145.414 kb on + strand, within IAI47_10130 at 2145.414 kb on + strand, within IAI47_10130 at 2145.414 kb on + strand, within IAI47_10130 at 2145.416 kb on + strand, within IAI47_10130 at 2145.416 kb on + strand, within IAI47_10130 at 2145.417 kb on - strand, within IAI47_10130 at 2145.417 kb on - strand, within IAI47_10130 at 2145.446 kb on + strand, within IAI47_10130 at 2145.549 kb on + strand at 2145.549 kb on + strand at 2145.549 kb on + strand at 2145.549 kb on + strand at 2145.549 kb on + strand at 2145.549 kb on + strand at 2145.550 kb on - strand at 2145.550 kb on - strand at 2145.550 kb on - strand at 2145.550 kb on - strand at 2145.550 kb on - strand at 2145.555 kb on + strand at 2145.556 kb on - strand at 2145.600 kb on - strand at 2145.640 kb on + strand at 2145.640 kb on + strand at 2145.640 kb on + strand at 2145.640 kb on + strand at 2145.641 kb on - strand at 2145.641 kb on - strand at 2145.641 kb on - strand at 2145.657 kb on + strand at 2145.658 kb on - strand at 2145.658 kb on - strand at 2145.701 kb on + strand, within IAI47_10135 at 2145.720 kb on + strand, within IAI47_10135 at 2145.735 kb on + strand, within IAI47_10135 at 2145.804 kb on + strand, within IAI47_10135 at 2145.960 kb on + strand, within IAI47_10135 at 2145.961 kb on - strand, within IAI47_10135 at 2145.982 kb on - strand, within IAI47_10135 at 2146.008 kb on + strand, within IAI47_10135 at 2146.009 kb on - strand, within IAI47_10135 at 2146.009 kb on - strand, within IAI47_10135 at 2146.040 kb on - strand, within IAI47_10135 at 2146.063 kb on + strand, within IAI47_10135 at 2146.063 kb on + strand, within IAI47_10135 at 2146.063 kb on + strand, within IAI47_10135 at 2146.063 kb on + strand, within IAI47_10135 at 2146.063 kb on + strand, within IAI47_10135 at 2146.063 kb on + strand, within IAI47_10135 at 2146.064 kb on - strand, within IAI47_10135 at 2146.064 kb on - strand, within IAI47_10135 at 2146.064 kb on - strand, within IAI47_10135 at 2146.064 kb on - strand, within IAI47_10135 at 2146.064 kb on - strand, within IAI47_10135 at 2146.064 kb on - strand, within IAI47_10135 at 2146.124 kb on + strand, within IAI47_10135 at 2146.167 kb on + strand, within IAI47_10135 at 2146.171 kb on + strand, within IAI47_10135 at 2146.171 kb on + strand, within IAI47_10135 at 2146.171 kb on + strand, within IAI47_10135 at 2146.171 kb on + strand, within IAI47_10135 at 2146.172 kb on - strand, within IAI47_10135 at 2146.172 kb on - strand, within IAI47_10135 at 2146.172 kb on - strand, within IAI47_10135 at 2146.255 kb on + strand, within IAI47_10135 at 2146.255 kb on + strand, within IAI47_10135 at 2146.255 kb on + strand, within IAI47_10135 at 2146.255 kb on + strand, within IAI47_10135 at 2146.256 kb on - strand, within IAI47_10135 at 2146.256 kb on - strand, within IAI47_10135 at 2146.256 kb on - strand, within IAI47_10135 at 2146.256 kb on - strand, within IAI47_10135 at 2146.256 kb on - strand, within IAI47_10135 at 2146.256 kb on - strand, within IAI47_10135 at 2146.413 kb on + strand at 2146.413 kb on + strand at 2146.413 kb on + strand at 2146.414 kb on - strand at 2146.414 kb on - strand at 2146.414 kb on - strand at 2146.414 kb on - strand at 2146.414 kb on - strand at 2146.450 kb on + strand at 2146.451 kb on - strand at 2146.545 kb on + strand, within IAI47_10140 at 2146.545 kb on + strand, within IAI47_10140 at 2146.545 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.609 kb on + strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.610 kb on - strand, within IAI47_10140 at 2146.654 kb on + strand, within IAI47_10140 at 2146.654 kb on + strand, within IAI47_10140 at 2146.655 kb on - strand, within IAI47_10140 at 2146.655 kb on - strand, within IAI47_10140 at 2146.685 kb on - strand, within IAI47_10140 at 2146.685 kb on - strand, within IAI47_10140 at 2146.702 kb on + strand, within IAI47_10140 at 2146.728 kb on + strand, within IAI47_10140 at 2146.729 kb on - strand, within IAI47_10140 at 2146.763 kb on + strand, within IAI47_10140 at 2146.794 kb on + strand, within IAI47_10140 at 2146.794 kb on + strand, within IAI47_10140 at 2146.794 kb on + strand, within IAI47_10140 at 2146.794 kb on + strand, within IAI47_10140 at 2146.794 kb on + strand, within IAI47_10140 at 2146.795 kb on - strand, within IAI47_10140 at 2146.795 kb on - strand, within IAI47_10140 at 2146.795 kb on - strand, within IAI47_10140 at 2146.890 kb on + strand, within IAI47_10140 at 2146.890 kb on + strand, within IAI47_10140 at 2146.891 kb on - strand, within IAI47_10140 at 2146.891 kb on - strand, within IAI47_10140 at 2146.891 kb on - strand, within IAI47_10140 at 2146.977 kb on + strand, within IAI47_10140 at 2146.977 kb on + strand, within IAI47_10140 at 2146.977 kb on + strand, within IAI47_10140 at 2146.978 kb on - strand, within IAI47_10140 at 2146.978 kb on - strand, within IAI47_10140 at 2146.980 kb on + strand, within IAI47_10140 at 2146.980 kb on + strand, within IAI47_10140 at 2146.981 kb on - strand, within IAI47_10140 at 2146.981 kb on - strand, within IAI47_10140 at 2146.981 kb on - strand, within IAI47_10140 at 2147.009 kb on - strand, within IAI47_10140 at 2147.152 kb on + strand, within IAI47_10140 at 2147.153 kb on - strand, within IAI47_10140 at 2147.153 kb on - strand, within IAI47_10140 at 2147.153 kb on - strand, within IAI47_10140 at 2147.153 kb on - strand, within IAI47_10140 at 2147.153 kb on - strand, within IAI47_10140 at 2147.153 kb on - strand, within IAI47_10140 at 2147.156 kb on - strand, within IAI47_10140
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,144,622 + IAI47_10130 0.25 +0.7 2,144,623 - IAI47_10130 0.25 -0.6 2,144,675 - IAI47_10130 0.29 -1.1 2,144,718 + IAI47_10130 0.32 -0.2 2,144,718 + IAI47_10130 0.32 -0.2 2,144,719 - IAI47_10130 0.32 -1.6 2,144,757 + IAI47_10130 0.35 +1.1 2,144,757 + IAI47_10130 0.35 +0.2 2,144,757 + IAI47_10130 0.35 +0.3 2,144,758 - IAI47_10130 0.35 +0.5 2,144,758 - IAI47_10130 0.35 -0.2 2,144,796 - IAI47_10130 0.38 -2.2 2,144,796 - IAI47_10130 0.38 -0.8 2,144,796 - IAI47_10130 0.38 +0.1 2,144,796 - IAI47_10130 0.38 -1.5 2,144,820 - IAI47_10130 0.40 +1.4 2,144,820 - IAI47_10130 0.40 +0.1 2,144,871 - IAI47_10130 0.44 +0.8 2,144,906 + IAI47_10130 0.46 +0.9 2,144,906 + IAI47_10130 0.46 +0.1 2,144,906 + IAI47_10130 0.46 +0.1 2,144,907 - IAI47_10130 0.46 +0.1 2,144,914 + IAI47_10130 0.47 +1.0 2,144,914 + IAI47_10130 0.47 -2.0 2,144,914 + IAI47_10130 0.47 -1.1 2,144,915 - IAI47_10130 0.47 +0.5 2,144,915 - IAI47_10130 0.47 +0.4 2,144,915 - IAI47_10130 0.47 +0.2 2,144,915 - IAI47_10130 0.47 +1.8 2,144,915 - IAI47_10130 0.47 +0.3 2,144,915 - IAI47_10130 0.47 -0.9 2,145,002 + IAI47_10130 0.54 -0.6 2,145,002 + IAI47_10130 0.54 -1.2 2,145,003 - IAI47_10130 0.54 +1.1 2,145,003 - IAI47_10130 0.54 -1.8 2,145,075 + IAI47_10130 0.59 -0.0 2,145,076 - IAI47_10130 0.59 -0.5 2,145,145 + IAI47_10130 0.65 +1.9 2,145,145 + IAI47_10130 0.65 -1.8 2,145,146 - IAI47_10130 0.65 -1.1 2,145,162 - IAI47_10130 0.66 -0.2 2,145,206 + IAI47_10130 0.69 +1.4 2,145,207 - IAI47_10130 0.69 +1.3 2,145,214 + IAI47_10130 0.70 +1.7 2,145,215 - IAI47_10130 0.70 +1.8 2,145,215 - IAI47_10130 0.70 -0.5 2,145,215 - IAI47_10130 0.70 -0.2 2,145,252 + IAI47_10130 0.73 +1.9 2,145,252 + IAI47_10130 0.73 +2.5 2,145,252 + IAI47_10130 0.73 +0.4 2,145,253 - IAI47_10130 0.73 +0.1 2,145,253 - IAI47_10130 0.73 +1.4 2,145,253 - IAI47_10130 0.73 -0.1 2,145,339 + IAI47_10130 0.80 -1.8 2,145,339 + IAI47_10130 0.80 +1.5 2,145,374 + IAI47_10130 0.82 +0.4 2,145,414 + IAI47_10130 0.85 +0.8 2,145,414 + IAI47_10130 0.85 +0.5 2,145,414 + IAI47_10130 0.85 -0.2 2,145,414 + IAI47_10130 0.85 -2.5 2,145,416 + IAI47_10130 0.85 -0.5 2,145,416 + IAI47_10130 0.85 +1.3 2,145,417 - IAI47_10130 0.86 +0.5 2,145,417 - IAI47_10130 0.86 -0.5 2,145,446 + IAI47_10130 0.88 +1.1 2,145,549 + -0.2 2,145,549 + +1.2 2,145,549 + +0.0 2,145,549 + -1.3 2,145,549 + -0.9 2,145,549 + -1.2 2,145,550 - -1.1 2,145,550 - -1.6 2,145,550 - -1.2 2,145,550 - -0.9 2,145,550 - +0.3 2,145,555 + +0.7 2,145,556 - +0.4 2,145,600 - -0.2 2,145,640 + +0.1 2,145,640 + -2.5 2,145,640 + -0.5 2,145,640 + -0.0 2,145,641 - +2.1 2,145,641 - -1.8 2,145,641 - -1.6 2,145,657 + -1.7 2,145,658 - -0.4 2,145,658 - +0.5 2,145,701 + IAI47_10135 0.10 +2.1 2,145,720 + IAI47_10135 0.13 +1.0 2,145,735 + IAI47_10135 0.15 +1.5 2,145,804 + IAI47_10135 0.23 -0.9 2,145,960 + IAI47_10135 0.42 -1.9 2,145,961 - IAI47_10135 0.43 +1.3 2,145,982 - IAI47_10135 0.45 -0.8 2,146,008 + IAI47_10135 0.48 +1.8 2,146,009 - IAI47_10135 0.49 +3.4 2,146,009 - IAI47_10135 0.49 +1.8 2,146,040 - IAI47_10135 0.52 +0.4 2,146,063 + IAI47_10135 0.55 -0.2 2,146,063 + IAI47_10135 0.55 +1.0 2,146,063 + IAI47_10135 0.55 +1.1 2,146,063 + IAI47_10135 0.55 -0.9 2,146,063 + IAI47_10135 0.55 -0.7 2,146,063 + IAI47_10135 0.55 +2.3 2,146,064 - IAI47_10135 0.55 -0.2 2,146,064 - IAI47_10135 0.55 +0.5 2,146,064 - IAI47_10135 0.55 +0.4 2,146,064 - IAI47_10135 0.55 +0.9 2,146,064 - IAI47_10135 0.55 -0.1 2,146,064 - IAI47_10135 0.55 +1.1 2,146,124 + IAI47_10135 0.63 +2.9 2,146,167 + IAI47_10135 0.68 -0.2 2,146,171 + IAI47_10135 0.69 -1.6 2,146,171 + IAI47_10135 0.69 -0.2 2,146,171 + IAI47_10135 0.69 +1.6 2,146,171 + IAI47_10135 0.69 -1.1 2,146,172 - IAI47_10135 0.69 +1.8 2,146,172 - IAI47_10135 0.69 -1.2 2,146,172 - IAI47_10135 0.69 +1.1 2,146,255 + IAI47_10135 0.79 -1.1 2,146,255 + IAI47_10135 0.79 -0.9 2,146,255 + IAI47_10135 0.79 +0.8 2,146,255 + IAI47_10135 0.79 +1.4 2,146,256 - IAI47_10135 0.79 +0.6 2,146,256 - IAI47_10135 0.79 +2.7 2,146,256 - IAI47_10135 0.79 +1.7 2,146,256 - IAI47_10135 0.79 +0.7 2,146,256 - IAI47_10135 0.79 -0.9 2,146,256 - IAI47_10135 0.79 -0.9 2,146,413 + +0.5 2,146,413 + -0.2 2,146,413 + -2.0 2,146,414 - +0.3 2,146,414 - -1.3 2,146,414 - -0.2 2,146,414 - +4.0 2,146,414 - +2.7 2,146,450 + -1.3 2,146,451 - +2.1 2,146,545 + IAI47_10140 0.16 -0.7 2,146,545 + IAI47_10140 0.16 +1.4 2,146,545 + IAI47_10140 0.16 +0.5 2,146,609 + IAI47_10140 0.23 -0.7 2,146,609 + IAI47_10140 0.23 -1.7 2,146,609 + IAI47_10140 0.23 -1.1 2,146,609 + IAI47_10140 0.23 -1.5 2,146,609 + IAI47_10140 0.23 -1.6 2,146,609 + IAI47_10140 0.23 -0.8 2,146,609 + IAI47_10140 0.23 +1.4 2,146,609 + IAI47_10140 0.23 +3.1 2,146,609 + IAI47_10140 0.23 +0.1 2,146,609 + IAI47_10140 0.23 -0.3 2,146,609 + IAI47_10140 0.23 -0.2 2,146,609 + IAI47_10140 0.23 -0.7 2,146,609 + IAI47_10140 0.23 -2.0 2,146,610 - IAI47_10140 0.23 +0.9 2,146,610 - IAI47_10140 0.23 +0.3 2,146,610 - IAI47_10140 0.23 -0.9 2,146,610 - IAI47_10140 0.23 -0.2 2,146,610 - IAI47_10140 0.23 +0.4 2,146,610 - IAI47_10140 0.23 +0.5 2,146,610 - IAI47_10140 0.23 +1.5 2,146,610 - IAI47_10140 0.23 -1.5 2,146,654 + IAI47_10140 0.28 +0.1 2,146,654 + IAI47_10140 0.28 -1.1 2,146,655 - IAI47_10140 0.28 -0.9 2,146,655 - IAI47_10140 0.28 -0.3 2,146,685 - IAI47_10140 0.32 -0.5 2,146,685 - IAI47_10140 0.32 -0.5 2,146,702 + IAI47_10140 0.34 -0.5 2,146,728 + IAI47_10140 0.36 -0.1 2,146,729 - IAI47_10140 0.37 +1.3 2,146,763 + IAI47_10140 0.40 -1.0 2,146,794 + IAI47_10140 0.44 -0.2 2,146,794 + IAI47_10140 0.44 +1.8 2,146,794 + IAI47_10140 0.44 +0.3 2,146,794 + IAI47_10140 0.44 +1.5 2,146,794 + IAI47_10140 0.44 +1.9 2,146,795 - IAI47_10140 0.44 -2.3 2,146,795 - IAI47_10140 0.44 -0.9 2,146,795 - IAI47_10140 0.44 +1.1 2,146,890 + IAI47_10140 0.54 -0.9 2,146,890 + IAI47_10140 0.54 -1.7 2,146,891 - IAI47_10140 0.55 +0.5 2,146,891 - IAI47_10140 0.55 +0.9 2,146,891 - IAI47_10140 0.55 +1.5 2,146,977 + IAI47_10140 0.64 -0.5 2,146,977 + IAI47_10140 0.64 +1.3 2,146,977 + IAI47_10140 0.64 +3.1 2,146,978 - IAI47_10140 0.64 +0.4 2,146,978 - IAI47_10140 0.64 +1.1 2,146,980 + IAI47_10140 0.64 -0.9 2,146,980 + IAI47_10140 0.64 -0.9 2,146,981 - IAI47_10140 0.65 +0.2 2,146,981 - IAI47_10140 0.65 -0.9 2,146,981 - IAI47_10140 0.65 +0.0 2,147,009 - IAI47_10140 0.68 +0.3 2,147,152 + IAI47_10140 0.84 +0.9 2,147,153 - IAI47_10140 0.84 -1.3 2,147,153 - IAI47_10140 0.84 -1.3 2,147,153 - IAI47_10140 0.84 +0.1 2,147,153 - IAI47_10140 0.84 -2.0 2,147,153 - IAI47_10140 0.84 -1.6 2,147,153 - IAI47_10140 0.84 -0.5 2,147,156 - IAI47_10140 0.84 +0.9
Or see this region's nucleotide sequence