Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS21590

Experiment: Temperature shift 30_to_4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_4
4,868,006	+		PFLU_RS21585	0.58	-0.3
4,868,006	+		PFLU_RS21585	0.58	+0.7
4,868,006	+		PFLU_RS21585	0.58	+0.6
4,868,006	+		PFLU_RS21585	0.58	-1.1
4,868,006	+		PFLU_RS21585	0.58	-0.6
4,868,006	+		PFLU_RS21585	0.58	+0.8
4,868,006	+		PFLU_RS21585	0.58	-0.9
4,868,007	-		PFLU_RS21585	0.58	-0.1
4,868,007	-		PFLU_RS21585	0.58	+2.1
4,868,007	-		PFLU_RS21585	0.58	+1.1
4,868,007	-		PFLU_RS21585	0.58	-0.5
4,868,007	-		PFLU_RS21585	0.58	+0.3
4,868,110	-		PFLU_RS21585	0.69	+2.4
4,868,111	+		PFLU_RS21585	0.69	-1.1
4,868,111	+		PFLU_RS21585	0.69	+1.9
4,868,111	+		PFLU_RS21585	0.69	-0.2
4,868,111	+		PFLU_RS21585	0.69	+0.6
4,868,112	-		PFLU_RS21585	0.69	+0.3
4,868,112	-		PFLU_RS21585	0.69	-0.4
4,868,377	+				-0.1
4,868,377	+				+0.6
4,868,377	+				-2.0
4,868,377	+				-0.0
4,868,377	+				-1.6
4,868,377	+				+1.2
4,868,377	+				+0.6
4,868,378	-				+1.0
4,868,378	-				-0.6
4,868,378	-				+0.9
4,868,402	-				+0.4
4,868,440	+				+0.4
4,868,440	+				+0.3
4,868,440	+				-0.9
4,868,440	+				-0.6
4,868,440	+				+0.4
4,868,440	+				-0.4
4,868,440	+				-2.8
4,868,440	+				-0.5
4,868,440	+				-0.2
4,868,440	+				+0.4
4,868,440	+				-0.8
4,868,440	+				-0.4
4,868,440	+				-1.3
4,868,440	+				-0.1
4,868,440	+				-0.3
4,868,440	+				+0.6
4,868,440	+				+0.4
4,868,441	-				+1.4
4,868,441	-				-1.0
4,868,441	-				-0.3
4,868,441	-				+1.8
4,868,441	-				-0.6
4,868,441	-				-1.0
4,868,441	-				-0.2
4,868,441	-				+0.3
4,868,441	-				-0.7
4,868,441	-				-0.2
4,868,441	-				-0.1
4,868,441	-				-0.4
4,868,441	-				-1.5
4,868,494	+				-1.2
4,868,494	+				+0.6
4,868,495	-				+0.1
4,868,495	-				-1.8
4,868,495	-				-0.6
4,868,495	-				+0.2
4,868,527	+				-0.2
4,868,528	-				-0.1
4,868,528	-				-0.8
4,868,556	+				+0.4
4,868,557	-				+0.2
4,868,648	-				+0.2
4,868,651	+				+0.6
4,868,651	+				-1.1
4,868,651	+				+0.4
4,868,651	+				-0.6
4,868,651	+				-1.1
4,868,651	+				-1.6
4,868,651	+				-0.0
4,868,651	+				-0.0
4,868,651	+				-0.6
4,868,651	+				+1.8
4,868,651	+				+0.0
4,868,651	+				+1.1
4,868,651	+				+1.4
4,868,651	+				+1.0
4,868,651	+				+0.6
4,868,652	-				-1.0
4,868,652	-				-0.5
4,868,652	-				-1.0
4,868,652	-				+0.4
4,868,652	-				+0.5
4,868,652	-				-0.2
4,868,652	-				+0.8
4,868,652	-				+0.3
4,868,652	-				-0.0
4,868,652	-				+0.4
4,868,652	-				+1.4
4,868,652	-				+1.4
4,868,652	-				-0.6
4,868,652	-				-2.0
4,868,654	+				+0.5
4,868,655	-				-1.6
4,868,661	+				+0.8
4,868,662	-				-0.2
4,868,662	-				-1.6
4,868,818	+				-2.0
4,868,818	+				-0.6
4,868,818	+				-0.6
4,868,818	+				+0.4
4,868,819	-				-0.2
4,868,819	-				-0.0
4,868,819	-				-1.0
4,868,819	-				-0.2
4,868,821	+				+1.1
4,868,821	+				+1.2
4,868,831	-				-0.6
4,868,831	-				-0.2
4,868,867	+				-2.2
4,868,867	+				-0.6
4,868,868	-				+1.2
4,868,878	+				+0.8
4,868,878	+				+0.1
4,868,879	-				-0.1
4,868,879	-				-0.3
4,868,879	-				+0.3
4,868,879	-				+0.4
4,869,153	+		PFLU_RS21590	0.47	-0.2
4,869,160	+		PFLU_RS21590	0.48	+0.9
4,869,160	+		PFLU_RS21590	0.48	+0.7
4,869,161	-		PFLU_RS21590	0.49	-0.6
4,869,323	+		PFLU_RS21590	0.85	-0.1
4,869,323	+		PFLU_RS21590	0.85	+0.4
4,869,324	-		PFLU_RS21590	0.85	-0.8
4,869,324	-		PFLU_RS21590	0.85	+0.4
4,869,324	-		PFLU_RS21590	0.85	+0.8
4,869,333	+		PFLU_RS21590	0.87	+0.4
4,869,333	+		PFLU_RS21590	0.87	+0.8
4,869,333	+		PFLU_RS21590	0.87	+0.4
4,869,334	-		PFLU_RS21590	0.87	-1.1
4,869,334	-		PFLU_RS21590	0.87	+0.5
4,869,334	-		PFLU_RS21590	0.87	-0.1
4,869,334	-		PFLU_RS21590	0.87	-0.5
4,869,334	-		PFLU_RS21590	0.87	+0.9
4,869,374	+				-0.9
4,869,374	+				+0.4
4,869,374	+				-0.6
4,869,374	+				-1.0
4,869,374	+				+1.7
4,869,374	+				-0.7
4,869,374	+				+1.8
4,869,375	-				+0.9
4,869,375	-				-0.1
4,869,375	-				-0.0
4,869,396	+				-2.3
4,869,406	+				+1.1
4,869,407	-				-1.3
4,869,407	-				+0.2
4,869,410	+				-0.5
4,869,410	+				-1.0
4,869,411	-				+0.3
4,869,423	-				+2.6
4,869,437	+				+0.0
4,869,437	+				-0.5
4,869,437	+				+0.2
4,869,437	+				-0.5
4,869,437	+				-1.4
4,869,437	+				+0.3
4,869,437	+				+0.7
4,869,437	+				-1.0
4,869,437	+				-0.4
4,869,437	+				-1.3
4,869,438	-				-0.2
4,869,438	-				-1.0
4,869,438	-				-0.4
4,869,438	-				-0.6
4,869,438	-				-0.5
4,869,438	-				+0.8
4,869,441	+				-0.3
4,869,441	+				+1.4
4,869,441	+				+1.4
4,869,441	+				-1.0
4,869,441	+				-0.8
4,869,441	+				-2.1
4,869,442	-				+0.5
4,869,442	-				+0.2
4,869,442	-				-0.4
4,869,442	-				+0.1
4,869,442	-				-2.1
4,869,442	-				+0.8
4,869,442	-				-0.1
4,869,442	-				-0.1
4,869,442	-				+0.7
4,869,442	-				+0.4
4,869,442	-				-1.1
4,869,444	+				-1.2
4,869,445	-				+0.1
4,869,445	-				-1.0
4,869,447	+				-2.8
4,869,447	+				+0.7
4,869,447	+				-0.5
4,869,447	+				+0.1
4,869,447	+				+0.6
4,869,447	+				-2.0
4,869,447	+				-0.1
4,869,447	+				-1.3
4,869,447	+				+0.2
4,869,447	+				+0.5
4,869,447	+				-1.1
4,869,447	+				+0.9
4,869,447	+				+0.0
4,869,447	+				-0.1
4,869,448	-				-0.4
4,869,448	-				+1.6
4,869,448	-				-0.5
4,869,448	-				-1.1
4,869,448	-				-0.9
4,869,448	-				+1.0
4,869,448	-				+0.8
4,869,448	-				-1.2
4,869,448	-				+0.5
4,869,448	-				+0.5
4,869,448	-				-0.0
4,869,448	-				+0.8
4,869,448	-				-0.2
4,869,448	-				-0.6
4,869,448	-				+0.9
4,869,448	-				-0.2
4,869,448	-				-1.2
4,869,448	-				-0.1
4,869,448	-				+1.4
4,869,448	-				+1.1
4,869,448	-				-0.3
4,869,448	-				-1.9
4,869,448	-				+1.8
4,869,448	-				-1.4
4,869,451	+				-0.5
4,869,451	+				-0.1
4,869,451	+				+0.4
4,869,451	+				-1.5
4,869,451	+				-2.0
4,869,451	+				+0.0
4,869,451	+				-1.0
4,869,451	+				+0.0
4,869,451	+				+0.0
4,869,451	+				-1.4
4,869,451	+				+1.9
4,869,451	+				+0.0
4,869,451	+				-0.6
4,869,451	+				-0.1
4,869,451	+				+2.4
4,869,451	+				-0.3
4,869,451	+				-2.8
4,869,452	-				+1.4
4,869,452	-				+1.8
4,869,452	-				-0.8
4,869,452	-				-1.3
4,869,452	-				+0.2
4,869,452	-				-0.5
4,869,452	-				+0.0
4,869,452	-				+0.5
4,869,452	-				-0.7
4,869,452	-				-0.1
4,869,452	-				+1.4
4,869,452	-				+0.8
4,869,452	-				+1.0
4,869,452	-				+0.7
4,869,452	-				+2.1
4,869,452	-				-2.0
4,869,452	-				-0.4
4,869,452	-				+0.1
4,869,452	-				+0.0
4,869,499	+				-0.3
4,869,499	+				+1.8
4,869,500	-				-1.2
4,869,500	-				-0.3
4,869,500	-				+0.4
4,869,505	+				+0.9
4,869,505	+				-0.2
4,869,505	+				+0.8
4,869,505	+				-0.5
4,869,505	+				-1.1
4,869,505	+				+1.0
4,869,505	+				+0.6
4,869,505	+				-0.1
4,869,506	-				-0.5
4,869,506	-				-0.6
4,869,506	-				+0.9
4,869,506	-				-0.5
4,869,506	-				-1.3
4,869,506	-				+0.4
4,869,506	-				+0.8
4,869,506	-				+0.4
4,869,506	-				+0.7
4,869,506	-				-0.5
4,869,506	-				+1.8
4,869,506	-				+1.4
4,869,506	-				+1.2
4,869,521	+				-0.3
4,869,521	+				+0.1
4,869,524	+				+0.8
4,869,524	+				+1.4
4,869,524	+				-1.0
4,869,524	+				-0.2
4,869,524	+				+1.4
4,869,525	-				+0.6
4,869,525	-				-0.2
4,869,525	-				-1.0
4,869,525	-				-0.3
4,869,526	+				-0.3
4,869,526	+				-0.5
4,869,526	+				-0.1
4,869,526	+				+0.8
4,869,526	+				-0.6
4,869,526	+				-0.2
4,869,526	+				+1.7
4,869,526	+				+1.0
4,869,526	+				+0.2
4,869,527	-				-0.8
4,869,527	-				-1.1
4,869,527	-				-0.1
4,869,527	-				-0.2
4,869,527	-				+1.4
4,869,527	-				+0.9
4,869,527	-				+0.9
4,869,527	-				-0.4
4,869,527	-				+1.4
4,869,527	-				+0.6
4,869,527	-				+0.2
4,869,543	-				+0.2
4,869,543	-				+0.6
4,869,556	-				+0.1
4,869,580	-				+0.2
4,869,617	+				+0.1
4,869,620	+				-0.1
4,869,620	+				+2.6
4,869,621	-				-0.3
4,869,621	-				-1.3
4,869,629	+				+0.0
4,869,629	+				-0.3
4,869,629	+				-1.2
4,869,629	+				-1.6
4,869,629	+				+0.0
4,869,630	-				+0.6
4,869,630	-				-1.6
4,869,630	-				-0.9
4,869,632	+				+0.5
4,869,632	+				-0.1
4,869,632	+				-0.3
4,869,632	+				-0.3
4,869,632	+				+1.4
4,869,632	+				+0.2
4,869,632	+				-0.9
4,869,632	+				-0.0
4,869,633	-				-0.1
4,869,633	-				-0.6
4,869,633	-				-1.0
4,869,633	-				+0.4
4,869,633	-				-0.2
4,869,633	-				-1.0
4,869,633	-				-1.2
4,869,633	-				-1.2
4,869,668	+				-0.3
4,869,668	+				+0.7
4,869,668	+				-0.5
4,869,668	+				+0.0
4,869,668	+				+0.5
4,869,668	+				-1.2
4,869,668	+				+0.2
4,869,668	+				+0.4
4,869,669	-				-1.8
4,869,669	-				+0.5
4,869,669	-				+0.9
4,869,669	-				-0.2
4,869,669	-				+0.7
4,869,669	-				+2.4
4,869,669	-				+0.4
4,869,669	-				-0.5
4,869,669	-				-0.7
4,869,669	-				-0.6
4,869,669	-				-1.2
4,869,672	+				+1.9
4,869,672	+				-0.1
4,869,672	+				+2.4
4,869,672	+				+1.1
4,869,672	+				-1.5
4,869,673	-				-1.0
4,869,692	+				+0.1
4,869,692	+				+0.5
4,869,693	-				+0.4
4,869,758	+				-1.4
4,869,759	-				-1.2
4,869,759	-				+0.8
4,869,759	-				-0.3
4,869,759	-				+0.1
4,869,759	-				-0.2
4,869,759	-				+0.8
4,869,762	+				+0.2
4,869,762	+				+1.7
4,869,762	+				-0.6
4,869,762	+				-0.2
4,869,762	+				+0.5
4,869,762	+				-2.3
4,869,762	+				-0.3
4,869,762	+				+0.5
4,869,762	+				+1.4
4,869,762	+				-0.6
4,869,762	+				+1.4
4,869,762	+				+0.2
4,869,762	+				-0.6
4,869,763	-				-1.0
4,869,763	-				-0.2
4,869,763	-				-2.0
4,869,763	-				+0.1
4,869,763	-				+0.6
4,869,763	-				-1.8
4,869,763	-				-0.6
4,869,763	-				+0.4
4,869,763	-				+1.1
4,869,763	-				-0.1
4,869,763	-				-0.5
4,869,763	-				+0.8
4,869,763	-				+1.2
4,869,763	-				+0.5
4,869,763	-				+0.9
4,869,763	-				+0.2
4,869,763	-				+0.7
4,869,763	-				+0.0
4,869,806	+				-2.9
4,869,806	+				+0.7
4,869,806	+				-1.1
4,869,806	+				+0.3
4,869,807	-				-1.0
4,869,807	-				-0.5
4,869,867	+		PFLU_RS21595	0.12	-0.2
4,869,867	+		PFLU_RS21595	0.12	+0.8
4,869,867	+		PFLU_RS21595	0.12	+0.4
4,869,868	-		PFLU_RS21595	0.12	-1.3
4,869,868	-		PFLU_RS21595	0.12	-0.7
4,869,868	-		PFLU_RS21595	0.12	+2.4
4,869,868	-		PFLU_RS21595	0.12	-0.6
4,869,869	+		PFLU_RS21595	0.12	+1.1
4,870,210	+		PFLU_RS21595	0.50	+0.0
4,870,210	+		PFLU_RS21595	0.50	-0.6
4,870,211	-		PFLU_RS21595	0.51	-2.1