Experiment: Formate with MreB Peturbing Compound A22 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02870 and MMP_RS02875 are separated by 33 nucleotides MMP_RS02875 and MMP_RS02880 are separated by 14 nucleotides MMP_RS02880 and MMP_RS02885 are separated by 26 nucleotides
MMP_RS02870: MMP_RS02870 - hypothetical protein, at 546,548 to 547,150
_RS02870
MMP_RS02875: MMP_RS02875 - chorismate pyruvate-lyase family protein, at 547,184 to 547,699
_RS02875
MMP_RS02880: MMP_RS02880 - AmmeMemoRadiSam system radical SAM enzyme, at 547,714 to 548,724
_RS02880
MMP_RS02885: MMP_RS02885 - molybdopterin biosynthesis protein, at 548,751 to 550,601
_RS02885
Position (kb) 547
548
549 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 546.784 kb on - strand, within MMP_RS02870 at 546.792 kb on + strand, within MMP_RS02870 at 546.865 kb on - strand, within MMP_RS02870 at 547.066 kb on + strand, within MMP_RS02870 at 547.074 kb on - strand, within MMP_RS02870 at 547.075 kb on + strand, within MMP_RS02870 at 547.095 kb on - strand at 547.107 kb on - strand at 547.123 kb on + strand at 547.123 kb on + strand at 547.161 kb on + strand at 547.173 kb on - strand at 547.183 kb on - strand at 547.192 kb on - strand at 547.236 kb on - strand, within MMP_RS02875 at 547.285 kb on - strand, within MMP_RS02875 at 547.289 kb on + strand, within MMP_RS02875 at 547.299 kb on - strand, within MMP_RS02875 at 547.341 kb on + strand, within MMP_RS02875 at 547.363 kb on + strand, within MMP_RS02875 at 547.367 kb on - strand, within MMP_RS02875 at 547.441 kb on + strand, within MMP_RS02875 at 547.454 kb on + strand, within MMP_RS02875 at 547.583 kb on + strand, within MMP_RS02875 at 547.628 kb on - strand, within MMP_RS02875 at 547.634 kb on + strand, within MMP_RS02875 at 547.634 kb on + strand, within MMP_RS02875 at 547.634 kb on + strand, within MMP_RS02875 at 547.635 kb on - strand, within MMP_RS02875 at 547.665 kb on - strand at 547.679 kb on + strand at 547.856 kb on - strand, within MMP_RS02880 at 547.920 kb on - strand, within MMP_RS02880 at 548.098 kb on - strand, within MMP_RS02880 at 548.099 kb on + strand, within MMP_RS02880 at 548.245 kb on + strand, within MMP_RS02880 at 548.246 kb on - strand, within MMP_RS02880 at 548.471 kb on + strand, within MMP_RS02880 at 548.501 kb on - strand, within MMP_RS02880 at 548.501 kb on - strand, within MMP_RS02880 at 548.501 kb on - strand, within MMP_RS02880 at 548.502 kb on + strand, within MMP_RS02880 at 548.555 kb on - strand, within MMP_RS02880 at 548.556 kb on + strand, within MMP_RS02880 at 548.556 kb on + strand, within MMP_RS02880 at 548.558 kb on + strand, within MMP_RS02880 at 548.596 kb on - strand, within MMP_RS02880 at 548.617 kb on + strand, within MMP_RS02880 at 548.665 kb on + strand at 548.718 kb on + strand at 548.718 kb on + strand at 548.737 kb on - strand at 548.738 kb on + strand at 548.757 kb on - strand at 548.758 kb on + strand at 548.761 kb on - strand at 548.762 kb on + strand at 548.762 kb on + strand at 548.786 kb on - strand at 548.876 kb on + strand at 549.091 kb on - strand, within MMP_RS02885 at 549.188 kb on + strand, within MMP_RS02885 at 549.203 kb on - strand, within MMP_RS02885 at 549.204 kb on + strand, within MMP_RS02885 at 549.204 kb on + strand, within MMP_RS02885 at 549.206 kb on + strand, within MMP_RS02885 at 549.224 kb on - strand, within MMP_RS02885 at 549.308 kb on + strand, within MMP_RS02885 at 549.328 kb on - strand, within MMP_RS02885 at 549.329 kb on + strand, within MMP_RS02885 at 549.357 kb on + strand, within MMP_RS02885 at 549.374 kb on - strand, within MMP_RS02885 at 549.689 kb on + strand, within MMP_RS02885 at 549.692 kb on + strand, within MMP_RS02885
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with MreB Peturbing Compound A22 0.25 mM remove 546,784 - MMP_RS02870 0.39 -0.1 546,792 + MMP_RS02870 0.40 +0.0 546,865 - MMP_RS02870 0.53 -0.1 547,066 + MMP_RS02870 0.86 +0.5 547,074 - MMP_RS02870 0.87 -0.0 547,075 + MMP_RS02870 0.87 +0.2 547,095 - +0.3 547,107 - -0.3 547,123 + +0.1 547,123 + +0.4 547,161 + -0.7 547,173 - -2.4 547,183 - -2.1 547,192 - -1.7 547,236 - MMP_RS02875 0.10 -2.5 547,285 - MMP_RS02875 0.20 -3.4 547,289 + MMP_RS02875 0.20 -2.0 547,299 - MMP_RS02875 0.22 -1.3 547,341 + MMP_RS02875 0.30 -2.0 547,363 + MMP_RS02875 0.35 -2.1 547,367 - MMP_RS02875 0.35 -1.8 547,441 + MMP_RS02875 0.50 -2.4 547,454 + MMP_RS02875 0.52 -1.5 547,583 + MMP_RS02875 0.77 -2.0 547,628 - MMP_RS02875 0.86 -2.8 547,634 + MMP_RS02875 0.87 -1.7 547,634 + MMP_RS02875 0.87 -0.8 547,634 + MMP_RS02875 0.87 -3.0 547,635 - MMP_RS02875 0.87 -2.8 547,665 - -3.7 547,679 + -1.5 547,856 - MMP_RS02880 0.14 -1.0 547,920 - MMP_RS02880 0.20 -0.4 548,098 - MMP_RS02880 0.38 -0.1 548,099 + MMP_RS02880 0.38 +0.2 548,245 + MMP_RS02880 0.53 +0.3 548,246 - MMP_RS02880 0.53 -0.8 548,471 + MMP_RS02880 0.75 -0.3 548,501 - MMP_RS02880 0.78 -1.8 548,501 - MMP_RS02880 0.78 -0.4 548,501 - MMP_RS02880 0.78 -1.1 548,502 + MMP_RS02880 0.78 +0.1 548,555 - MMP_RS02880 0.83 -1.1 548,556 + MMP_RS02880 0.83 +0.1 548,556 + MMP_RS02880 0.83 -0.5 548,558 + MMP_RS02880 0.83 -0.5 548,596 - MMP_RS02880 0.87 -0.1 548,617 + MMP_RS02880 0.89 -0.5 548,665 + +0.1 548,718 + -0.3 548,718 + +0.3 548,737 - -0.5 548,738 + +0.3 548,757 - -2.4 548,758 + -1.3 548,761 - -1.6 548,762 + -1.8 548,762 + -0.9 548,786 - -1.4 548,876 + -1.6 549,091 - MMP_RS02885 0.18 -1.7 549,188 + MMP_RS02885 0.24 -1.9 549,203 - MMP_RS02885 0.24 -1.5 549,204 + MMP_RS02885 0.24 -2.3 549,204 + MMP_RS02885 0.24 -0.7 549,206 + MMP_RS02885 0.25 -1.5 549,224 - MMP_RS02885 0.26 -1.2 549,308 + MMP_RS02885 0.30 -1.8 549,328 - MMP_RS02885 0.31 -1.4 549,329 + MMP_RS02885 0.31 -0.6 549,357 + MMP_RS02885 0.33 -1.0 549,374 - MMP_RS02885 0.34 -1.2 549,689 + MMP_RS02885 0.51 -1.0 549,692 + MMP_RS02885 0.51 -2.0
Or see this region's nucleotide sequence