Experiment: Formate with Lithium chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02275 and MMP_RS02280 are separated by 259 nucleotides MMP_RS02280 and MMP_RS02285 are separated by 54 nucleotides MMP_RS02285 and MMP_RS02290 are separated by 62 nucleotides
MMP_RS02275: MMP_RS02275 - Ni-sirohydrochlorin a c-diamide reductive cyclase catalytic subunit, at 429,261 to 430,361
_RS02275
MMP_RS02280: MMP_RS02280 - DUF211 domain-containing protein, at 430,621 to 430,914
_RS02280
MMP_RS02285: MMP_RS02285 - RNase P subunit p30 family protein, at 430,969 to 431,667
_RS02285
MMP_RS02290: MMP_RS02290 - MBL fold metallo-hydrolase, at 431,730 to 433,031
_RS02290
Position (kb)
430
431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 429.829 kb on - strand at 430.009 kb on + strand at 430.018 kb on + strand at 430.021 kb on + strand at 430.155 kb on - strand at 430.378 kb on + strand at 430.405 kb on - strand at 430.413 kb on + strand at 430.458 kb on + strand at 430.459 kb on - strand at 430.485 kb on + strand at 430.506 kb on - strand at 430.506 kb on - strand at 430.509 kb on + strand at 430.510 kb on - strand at 430.511 kb on + strand at 430.511 kb on + strand at 430.516 kb on + strand at 430.530 kb on + strand at 430.530 kb on + strand at 430.531 kb on - strand at 430.532 kb on + strand at 430.532 kb on + strand at 430.532 kb on + strand at 430.535 kb on - strand at 430.537 kb on - strand at 430.542 kb on + strand at 430.542 kb on + strand at 430.544 kb on - strand at 430.545 kb on + strand at 430.602 kb on - strand at 430.602 kb on - strand at 430.603 kb on + strand at 430.819 kb on + strand, within MMP_RS02280 at 430.906 kb on + strand at 430.906 kb on + strand at 430.906 kb on + strand at 430.909 kb on - strand at 430.938 kb on - strand at 430.941 kb on - strand at 431.138 kb on + strand at 431.391 kb on + strand at 431.409 kb on - strand at 431.500 kb on + strand at 431.504 kb on + strand at 431.755 kb on + strand at 431.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Lithium chloride 250 mM remove 429,829 - -0.9 430,009 + -1.3 430,018 + -2.2 430,021 + -1.2 430,155 - -2.3 430,378 + -0.9 430,405 - -0.1 430,413 + -0.2 430,458 + -0.6 430,459 - -0.3 430,485 + +0.5 430,506 - -0.3 430,506 - -0.1 430,509 + +0.1 430,510 - -0.1 430,511 + -0.1 430,511 + -0.0 430,516 + -0.6 430,530 + +0.2 430,530 + +0.6 430,531 - -0.2 430,532 + -0.5 430,532 + +0.4 430,532 + -0.1 430,535 - +0.7 430,537 - +0.1 430,542 + -1.3 430,542 + -0.6 430,544 - -2.3 430,545 + -0.5 430,602 - -1.2 430,602 - -0.2 430,603 + -0.2 430,819 + MMP_RS02280 0.67 -1.1 430,906 + -1.2 430,906 + -1.4 430,906 + -2.3 430,909 - -1.7 430,938 - -0.9 430,941 - -2.2 431,138 + -3.1 431,391 + -3.8 431,409 - -0.9 431,500 + -2.7 431,504 + -1.5 431,755 + -0.2 431,832 - -0.0
Or see this region's nucleotide sequence