Experiment: Formate with Lithium chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01315 and MMP_RS01320 are separated by 1 nucleotides MMP_RS01320 and MMP_RS01325 are separated by 12 nucleotides MMP_RS01325 and MMP_RS01330 overlap by 8 nucleotides MMP_RS01330 and MMP_RS01335 are separated by 37 nucleotides MMP_RS01335 and MMP_RS01340 are separated by 57 nucleotides MMP_RS01340 and MMP_RS01345 are separated by 7 nucleotides
MMP_RS01315: MMP_RS01315 - DUF2341 domain-containing protein, at 246,337 to 248,100
_RS01315
MMP_RS01320: MMP_RS01320 - hypothetical protein, at 248,102 to 248,371
_RS01320
MMP_RS01325: MMP_RS01325 - dihydroneopterin aldolase family protein, at 248,384 to 248,758
_RS01325
MMP_RS01330: MMP_RS01330 - DHH family phosphoesterase, at 248,751 to 249,767
_RS01330
MMP_RS01335: MMP_RS01335 - prefoldin subunit beta, at 249,805 to 250,146
_RS01335
MMP_RS01340: MMP_RS01340 - KEOPS complex Pcc1-like subunit, at 250,204 to 250,455
_RS01340
MMP_RS01345: MMP_RS01345 - rRNA maturation protein, at 250,463 to 250,957
_RS01345
Position (kb)
248
249
250 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 247.757 kb on - strand, within MMP_RS01315 at 247.782 kb on - strand, within MMP_RS01315 at 247.786 kb on - strand, within MMP_RS01315 at 247.786 kb on - strand, within MMP_RS01315 at 247.790 kb on - strand, within MMP_RS01315 at 247.791 kb on + strand, within MMP_RS01315 at 247.816 kb on - strand, within MMP_RS01315 at 247.819 kb on - strand, within MMP_RS01315 at 247.819 kb on - strand, within MMP_RS01315 at 247.820 kb on + strand, within MMP_RS01315 at 247.822 kb on - strand, within MMP_RS01315 at 247.822 kb on - strand, within MMP_RS01315 at 247.822 kb on - strand, within MMP_RS01315 at 247.822 kb on - strand, within MMP_RS01315 at 247.822 kb on - strand, within MMP_RS01315 at 247.823 kb on + strand, within MMP_RS01315 at 247.823 kb on + strand, within MMP_RS01315 at 247.823 kb on + strand, within MMP_RS01315 at 247.823 kb on + strand, within MMP_RS01315 at 247.823 kb on + strand, within MMP_RS01315 at 247.852 kb on - strand, within MMP_RS01315 at 247.852 kb on - strand, within MMP_RS01315 at 247.852 kb on - strand, within MMP_RS01315 at 247.853 kb on + strand, within MMP_RS01315 at 247.904 kb on - strand, within MMP_RS01315 at 247.905 kb on + strand, within MMP_RS01315 at 247.908 kb on - strand, within MMP_RS01315 at 247.909 kb on + strand, within MMP_RS01315 at 247.925 kb on + strand at 247.927 kb on - strand at 247.927 kb on - strand at 247.929 kb on - strand at 247.929 kb on - strand at 247.929 kb on - strand at 247.930 kb on + strand at 247.931 kb on - strand at 247.931 kb on - strand at 247.931 kb on - strand at 247.932 kb on + strand at 247.932 kb on + strand at 247.992 kb on - strand at 248.018 kb on - strand at 248.047 kb on - strand at 248.081 kb on - strand at 248.143 kb on - strand, within MMP_RS01320 at 248.218 kb on - strand, within MMP_RS01320 at 248.259 kb on + strand, within MMP_RS01320 at 248.261 kb on - strand, within MMP_RS01320 at 248.272 kb on - strand, within MMP_RS01320 at 248.303 kb on - strand, within MMP_RS01320 at 248.371 kb on - strand at 248.400 kb on - strand at 248.400 kb on - strand at 248.405 kb on - strand at 248.435 kb on - strand, within MMP_RS01325 at 248.469 kb on + strand, within MMP_RS01325 at 248.480 kb on - strand, within MMP_RS01325 at 248.486 kb on - strand, within MMP_RS01325 at 248.793 kb on + strand at 248.973 kb on - strand, within MMP_RS01330 at 248.987 kb on - strand, within MMP_RS01330 at 249.032 kb on - strand, within MMP_RS01330 at 249.033 kb on + strand, within MMP_RS01330 at 249.036 kb on - strand, within MMP_RS01330 at 249.036 kb on - strand, within MMP_RS01330 at 249.090 kb on + strand, within MMP_RS01330 at 249.094 kb on - strand, within MMP_RS01330 at 249.094 kb on - strand, within MMP_RS01330 at 249.094 kb on - strand, within MMP_RS01330 at 249.095 kb on + strand, within MMP_RS01330 at 249.095 kb on + strand, within MMP_RS01330 at 249.098 kb on - strand, within MMP_RS01330 at 249.126 kb on + strand, within MMP_RS01330 at 249.181 kb on - strand, within MMP_RS01330 at 249.201 kb on - strand, within MMP_RS01330 at 249.201 kb on - strand, within MMP_RS01330 at 249.202 kb on + strand, within MMP_RS01330 at 249.202 kb on + strand, within MMP_RS01330 at 249.203 kb on - strand, within MMP_RS01330 at 249.203 kb on - strand, within MMP_RS01330 at 249.225 kb on - strand, within MMP_RS01330 at 249.230 kb on - strand, within MMP_RS01330 at 249.231 kb on + strand, within MMP_RS01330 at 249.267 kb on + strand, within MMP_RS01330 at 249.267 kb on + strand, within MMP_RS01330 at 249.271 kb on - strand, within MMP_RS01330 at 249.437 kb on - strand, within MMP_RS01330 at 249.449 kb on - strand, within MMP_RS01330 at 249.450 kb on + strand, within MMP_RS01330 at 249.534 kb on - strand, within MMP_RS01330 at 249.534 kb on - strand, within MMP_RS01330 at 249.534 kb on - strand, within MMP_RS01330 at 249.555 kb on - strand, within MMP_RS01330 at 249.588 kb on - strand, within MMP_RS01330 at 249.604 kb on + strand, within MMP_RS01330 at 249.714 kb on - strand at 249.790 kb on - strand at 249.806 kb on - strand at 249.854 kb on - strand, within MMP_RS01335 at 249.891 kb on - strand, within MMP_RS01335 at 250.181 kb on + strand at 250.192 kb on + strand at 250.202 kb on - strand at 250.206 kb on + strand at 250.226 kb on - strand at 250.226 kb on - strand at 250.227 kb on + strand at 250.260 kb on + strand at 250.265 kb on - strand at 250.458 kb on - strand at 250.603 kb on + strand at 250.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Lithium chloride 250 mM remove 247,757 - MMP_RS01315 0.80 +1.3 247,782 - MMP_RS01315 0.82 +0.2 247,786 - MMP_RS01315 0.82 +0.7 247,786 - MMP_RS01315 0.82 +0.4 247,790 - MMP_RS01315 0.82 +0.1 247,791 + MMP_RS01315 0.82 +0.4 247,816 - MMP_RS01315 0.84 +0.0 247,819 - MMP_RS01315 0.84 +0.3 247,819 - MMP_RS01315 0.84 +0.5 247,820 + MMP_RS01315 0.84 -0.0 247,822 - MMP_RS01315 0.84 -0.0 247,822 - MMP_RS01315 0.84 +0.4 247,822 - MMP_RS01315 0.84 +0.4 247,822 - MMP_RS01315 0.84 -1.0 247,822 - MMP_RS01315 0.84 -0.1 247,823 + MMP_RS01315 0.84 +0.3 247,823 + MMP_RS01315 0.84 +0.5 247,823 + MMP_RS01315 0.84 -0.9 247,823 + MMP_RS01315 0.84 +0.3 247,823 + MMP_RS01315 0.84 +0.7 247,852 - MMP_RS01315 0.86 -0.3 247,852 - MMP_RS01315 0.86 -0.3 247,852 - MMP_RS01315 0.86 +0.4 247,853 + MMP_RS01315 0.86 +0.5 247,904 - MMP_RS01315 0.89 +0.9 247,905 + MMP_RS01315 0.89 +0.0 247,908 - MMP_RS01315 0.89 +0.1 247,909 + MMP_RS01315 0.89 -0.0 247,925 + +0.1 247,927 - -0.7 247,927 - +0.3 247,929 - +0.5 247,929 - -0.6 247,929 - +0.1 247,930 + +0.0 247,931 - +0.1 247,931 - -0.5 247,931 - -0.3 247,932 + -0.3 247,932 + +0.3 247,992 - +0.2 248,018 - +0.2 248,047 - -0.2 248,081 - -0.4 248,143 - MMP_RS01320 0.15 +0.4 248,218 - MMP_RS01320 0.43 +0.0 248,259 + MMP_RS01320 0.58 +1.3 248,261 - MMP_RS01320 0.59 +0.0 248,272 - MMP_RS01320 0.63 +0.1 248,303 - MMP_RS01320 0.74 -0.4 248,371 - -0.5 248,400 - -2.4 248,400 - -1.2 248,405 - -1.7 248,435 - MMP_RS01325 0.14 -1.4 248,469 + MMP_RS01325 0.23 -1.0 248,480 - MMP_RS01325 0.26 -4.0 248,486 - MMP_RS01325 0.27 -1.2 248,793 + -1.3 248,973 - MMP_RS01330 0.22 -0.5 248,987 - MMP_RS01330 0.23 -1.1 249,032 - MMP_RS01330 0.28 -0.5 249,033 + MMP_RS01330 0.28 -1.6 249,036 - MMP_RS01330 0.28 -1.0 249,036 - MMP_RS01330 0.28 -0.5 249,090 + MMP_RS01330 0.33 -1.1 249,094 - MMP_RS01330 0.34 -1.0 249,094 - MMP_RS01330 0.34 -0.0 249,094 - MMP_RS01330 0.34 +0.0 249,095 + MMP_RS01330 0.34 -1.0 249,095 + MMP_RS01330 0.34 -1.5 249,098 - MMP_RS01330 0.34 -0.6 249,126 + MMP_RS01330 0.37 -0.1 249,181 - MMP_RS01330 0.42 -0.1 249,201 - MMP_RS01330 0.44 -0.5 249,201 - MMP_RS01330 0.44 -0.7 249,202 + MMP_RS01330 0.44 -1.1 249,202 + MMP_RS01330 0.44 -4.1 249,203 - MMP_RS01330 0.44 -1.1 249,203 - MMP_RS01330 0.44 +0.1 249,225 - MMP_RS01330 0.47 -0.7 249,230 - MMP_RS01330 0.47 -0.2 249,231 + MMP_RS01330 0.47 -0.7 249,267 + MMP_RS01330 0.51 -1.0 249,267 + MMP_RS01330 0.51 -0.8 249,271 - MMP_RS01330 0.51 -1.0 249,437 - MMP_RS01330 0.67 -0.2 249,449 - MMP_RS01330 0.69 -1.4 249,450 + MMP_RS01330 0.69 -0.8 249,534 - MMP_RS01330 0.77 -0.6 249,534 - MMP_RS01330 0.77 -1.0 249,534 - MMP_RS01330 0.77 -0.6 249,555 - MMP_RS01330 0.79 -0.6 249,588 - MMP_RS01330 0.82 -1.2 249,604 + MMP_RS01330 0.84 -0.6 249,714 - -0.5 249,790 - -0.7 249,806 - -0.4 249,854 - MMP_RS01335 0.14 -1.0 249,891 - MMP_RS01335 0.25 -0.6 250,181 + +0.2 250,192 + -0.3 250,202 - -0.1 250,206 + -1.1 250,226 - -1.9 250,226 - -1.5 250,227 + -2.1 250,260 + -2.6 250,265 - -3.2 250,458 - -2.2 250,603 + -1.1 250,606 - +0.4
Or see this region's nucleotide sequence