Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20650 and IAI47_20655 are separated by 9 nucleotides IAI47_20655 and IAI47_20660 overlap by 14 nucleotides
IAI47_20650: IAI47_20650 - polyprenyl synthetase family protein, at 379,733 to 380,641
_20650
IAI47_20655: IAI47_20655 - glycosyltransferase family 1 protein, at 380,651 to 381,946
_20655
IAI47_20660: IAI47_20660 - lycopene beta-cyclase CrtY, at 381,933 to 383,093
_20660
Position (kb) 380
381
382 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 379.715 kb on + strand at 379.715 kb on + strand at 379.716 kb on - strand at 379.716 kb on - strand at 379.720 kb on - strand at 379.720 kb on - strand at 379.720 kb on - strand at 379.824 kb on + strand, within IAI47_20650 at 379.905 kb on + strand, within IAI47_20650 at 379.906 kb on - strand, within IAI47_20650 at 380.075 kb on + strand, within IAI47_20650 at 380.076 kb on - strand, within IAI47_20650 at 380.119 kb on + strand, within IAI47_20650 at 380.202 kb on + strand, within IAI47_20650 at 380.202 kb on + strand, within IAI47_20650 at 380.203 kb on - strand, within IAI47_20650 at 380.206 kb on + strand, within IAI47_20650 at 380.206 kb on + strand, within IAI47_20650 at 380.207 kb on - strand, within IAI47_20650 at 380.207 kb on - strand, within IAI47_20650 at 380.207 kb on - strand, within IAI47_20650 at 380.296 kb on + strand, within IAI47_20650 at 380.296 kb on + strand, within IAI47_20650 at 380.296 kb on + strand, within IAI47_20650 at 380.297 kb on - strand, within IAI47_20650 at 380.297 kb on - strand, within IAI47_20650 at 380.428 kb on + strand, within IAI47_20650 at 380.428 kb on + strand, within IAI47_20650 at 380.428 kb on + strand, within IAI47_20650 at 380.476 kb on + strand, within IAI47_20650 at 380.477 kb on - strand, within IAI47_20650 at 380.582 kb on + strand at 380.639 kb on + strand at 380.640 kb on - strand at 380.640 kb on - strand at 380.661 kb on - strand at 380.661 kb on - strand at 380.684 kb on + strand at 380.684 kb on + strand at 380.685 kb on - strand at 380.686 kb on + strand at 380.713 kb on + strand at 380.714 kb on - strand at 380.714 kb on - strand at 380.724 kb on + strand at 380.746 kb on + strand at 380.746 kb on + strand at 380.747 kb on - strand at 380.747 kb on - strand at 380.747 kb on - strand at 380.923 kb on + strand, within IAI47_20655 at 380.924 kb on - strand, within IAI47_20655 at 380.924 kb on - strand, within IAI47_20655 at 381.009 kb on + strand, within IAI47_20655 at 381.010 kb on - strand, within IAI47_20655 at 381.010 kb on - strand, within IAI47_20655 at 381.107 kb on + strand, within IAI47_20655 at 381.108 kb on - strand, within IAI47_20655 at 381.108 kb on - strand, within IAI47_20655 at 381.220 kb on - strand, within IAI47_20655 at 381.278 kb on - strand, within IAI47_20655 at 381.380 kb on + strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.454 kb on + strand, within IAI47_20655 at 381.496 kb on - strand, within IAI47_20655 at 381.655 kb on + strand, within IAI47_20655 at 381.758 kb on - strand, within IAI47_20655 at 381.758 kb on - strand, within IAI47_20655 at 381.758 kb on - strand, within IAI47_20655 at 381.812 kb on + strand, within IAI47_20655 at 381.812 kb on + strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.919 kb on + strand at 381.919 kb on + strand at 381.919 kb on + strand at 381.919 kb on + strand at 381.919 kb on + strand at 381.920 kb on - strand at 381.920 kb on - strand at 381.920 kb on - strand at 381.932 kb on + strand at 381.932 kb on + strand at 381.932 kb on + strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.950 kb on + strand at 381.951 kb on - strand at 381.951 kb on - strand at 381.951 kb on - strand at 381.951 kb on - strand at 382.030 kb on + strand at 382.030 kb on + strand at 382.031 kb on - strand at 382.316 kb on - strand, within IAI47_20660 at 382.350 kb on + strand, within IAI47_20660 at 382.350 kb on + strand, within IAI47_20660 at 382.374 kb on - strand, within IAI47_20660 at 382.489 kb on - strand, within IAI47_20660 at 382.489 kb on - strand, within IAI47_20660 at 382.512 kb on - strand, within IAI47_20660 at 382.529 kb on + strand, within IAI47_20660 at 382.530 kb on - strand, within IAI47_20660 at 382.530 kb on - strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.538 kb on + strand, within IAI47_20660 at 382.538 kb on + strand, within IAI47_20660 at 382.538 kb on + strand, within IAI47_20660 at 382.539 kb on - strand, within IAI47_20660 at 382.539 kb on - strand, within IAI47_20660 at 382.581 kb on - strand, within IAI47_20660 at 382.590 kb on + strand, within IAI47_20660 at 382.590 kb on + strand, within IAI47_20660 at 382.591 kb on - strand, within IAI47_20660 at 382.625 kb on - strand, within IAI47_20660 at 382.625 kb on - strand, within IAI47_20660 at 382.625 kb on - strand, within IAI47_20660 at 382.656 kb on - strand, within IAI47_20660 at 382.656 kb on - strand, within IAI47_20660 at 382.700 kb on - strand, within IAI47_20660 at 382.736 kb on + strand, within IAI47_20660 at 382.736 kb on + strand, within IAI47_20660 at 382.737 kb on - strand, within IAI47_20660 at 382.746 kb on + strand, within IAI47_20660 at 382.747 kb on - strand, within IAI47_20660 at 382.803 kb on - strand, within IAI47_20660 at 382.880 kb on - strand, within IAI47_20660 at 382.928 kb on + strand, within IAI47_20660 at 382.941 kb on + strand, within IAI47_20660
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 379,715 + -2.8 379,715 + +2.1 379,716 - -0.5 379,716 - +0.5 379,720 - +2.5 379,720 - +1.0 379,720 - -2.2 379,824 + IAI47_20650 0.10 -0.1 379,905 + IAI47_20650 0.19 +0.2 379,906 - IAI47_20650 0.19 +0.5 380,075 + IAI47_20650 0.38 -1.8 380,076 - IAI47_20650 0.38 +2.3 380,119 + IAI47_20650 0.42 -1.8 380,202 + IAI47_20650 0.52 -1.7 380,202 + IAI47_20650 0.52 -0.9 380,203 - IAI47_20650 0.52 -2.5 380,206 + IAI47_20650 0.52 -3.0 380,206 + IAI47_20650 0.52 -0.8 380,207 - IAI47_20650 0.52 -1.3 380,207 - IAI47_20650 0.52 +0.6 380,207 - IAI47_20650 0.52 +1.7 380,296 + IAI47_20650 0.62 -1.8 380,296 + IAI47_20650 0.62 +0.3 380,296 + IAI47_20650 0.62 +1.7 380,297 - IAI47_20650 0.62 +0.2 380,297 - IAI47_20650 0.62 -0.1 380,428 + IAI47_20650 0.76 -1.1 380,428 + IAI47_20650 0.76 -1.8 380,428 + IAI47_20650 0.76 -1.3 380,476 + IAI47_20650 0.82 -2.1 380,477 - IAI47_20650 0.82 -1.3 380,582 + -1.7 380,639 + -0.8 380,640 - -1.1 380,640 - -2.0 380,661 - +3.6 380,661 - +0.6 380,684 + -1.7 380,684 + -2.0 380,685 - +0.3 380,686 + +0.1 380,713 + +0.6 380,714 - +0.5 380,714 - +1.3 380,724 + +0.3 380,746 + -1.5 380,746 + +1.1 380,747 - -0.5 380,747 - +0.9 380,747 - -1.5 380,923 + IAI47_20655 0.21 +0.6 380,924 - IAI47_20655 0.21 +2.0 380,924 - IAI47_20655 0.21 -2.6 381,009 + IAI47_20655 0.28 +2.3 381,010 - IAI47_20655 0.28 +0.5 381,010 - IAI47_20655 0.28 -0.3 381,107 + IAI47_20655 0.35 +2.4 381,108 - IAI47_20655 0.35 -1.2 381,108 - IAI47_20655 0.35 -0.5 381,220 - IAI47_20655 0.44 -2.0 381,278 - IAI47_20655 0.48 -2.2 381,380 + IAI47_20655 0.56 -0.5 381,381 - IAI47_20655 0.56 -0.3 381,381 - IAI47_20655 0.56 +1.3 381,381 - IAI47_20655 0.56 +0.1 381,454 + IAI47_20655 0.62 -1.5 381,496 - IAI47_20655 0.65 -1.1 381,655 + IAI47_20655 0.77 -1.7 381,758 - IAI47_20655 0.85 -0.4 381,758 - IAI47_20655 0.85 -0.4 381,758 - IAI47_20655 0.85 +1.2 381,812 + IAI47_20655 0.90 +2.6 381,812 + IAI47_20655 0.90 -0.1 381,813 - IAI47_20655 0.90 -0.6 381,813 - IAI47_20655 0.90 +1.2 381,813 - IAI47_20655 0.90 +1.6 381,813 - IAI47_20655 0.90 -2.4 381,813 - IAI47_20655 0.90 -1.1 381,813 - IAI47_20655 0.90 +0.3 381,813 - IAI47_20655 0.90 -1.3 381,919 + +1.3 381,919 + -0.5 381,919 + +0.5 381,919 + -0.5 381,919 + +3.8 381,920 - +0.3 381,920 - -1.4 381,920 - +0.9 381,932 + +2.1 381,932 + -1.4 381,932 + -2.0 381,933 - +0.7 381,933 - -1.3 381,933 - +1.5 381,933 - -2.2 381,933 - -2.4 381,933 - +0.8 381,950 + +0.2 381,951 - -0.3 381,951 - +0.6 381,951 - +0.0 381,951 - -0.8 382,030 + +0.4 382,030 + +0.3 382,031 - +1.2 382,316 - IAI47_20660 0.33 -0.4 382,350 + IAI47_20660 0.36 -0.8 382,350 + IAI47_20660 0.36 +0.8 382,374 - IAI47_20660 0.38 +0.5 382,489 - IAI47_20660 0.48 -3.0 382,489 - IAI47_20660 0.48 +0.7 382,512 - IAI47_20660 0.50 -1.7 382,529 + IAI47_20660 0.51 +0.9 382,530 - IAI47_20660 0.51 -0.5 382,530 - IAI47_20660 0.51 +0.3 382,536 + IAI47_20660 0.52 -1.5 382,536 + IAI47_20660 0.52 -1.2 382,536 + IAI47_20660 0.52 -0.0 382,536 + IAI47_20660 0.52 -0.1 382,536 + IAI47_20660 0.52 -1.5 382,536 + IAI47_20660 0.52 -1.5 382,536 + IAI47_20660 0.52 +2.3 382,536 + IAI47_20660 0.52 +2.2 382,536 + IAI47_20660 0.52 -2.0 382,537 - IAI47_20660 0.52 -1.3 382,537 - IAI47_20660 0.52 -0.3 382,537 - IAI47_20660 0.52 -0.5 382,537 - IAI47_20660 0.52 -1.1 382,537 - IAI47_20660 0.52 -0.8 382,537 - IAI47_20660 0.52 +0.0 382,538 + IAI47_20660 0.52 -0.4 382,538 + IAI47_20660 0.52 +0.8 382,538 + IAI47_20660 0.52 -0.9 382,539 - IAI47_20660 0.52 +2.2 382,539 - IAI47_20660 0.52 -0.2 382,581 - IAI47_20660 0.56 -0.3 382,590 + IAI47_20660 0.57 +2.4 382,590 + IAI47_20660 0.57 +0.1 382,591 - IAI47_20660 0.57 +2.1 382,625 - IAI47_20660 0.60 -1.8 382,625 - IAI47_20660 0.60 -1.4 382,625 - IAI47_20660 0.60 +2.5 382,656 - IAI47_20660 0.62 -1.6 382,656 - IAI47_20660 0.62 -0.7 382,700 - IAI47_20660 0.66 +0.6 382,736 + IAI47_20660 0.69 -2.4 382,736 + IAI47_20660 0.69 +1.3 382,737 - IAI47_20660 0.69 -0.8 382,746 + IAI47_20660 0.70 +1.7 382,747 - IAI47_20660 0.70 +0.5 382,803 - IAI47_20660 0.75 +2.1 382,880 - IAI47_20660 0.82 -0.7 382,928 + IAI47_20660 0.86 +3.2 382,941 + IAI47_20660 0.87 +0.3
Or see this region's nucleotide sequence