Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_05300 and IAI47_05305 are separated by 1 nucleotides IAI47_05305 and IAI47_05310 are separated by 580 nucleotides
IAI47_05300: IAI47_05300 - succinyl-diaminopimelate desuccinylase, at 1,119,480 to 1,120,607
_05300
IAI47_05305: IAI47_05305 - ArsC family reductase, at 1,120,609 to 1,120,977
_05305
IAI47_05310: IAI47_05310 - multidrug efflux RND transporter permease AcrD, at 1,121,558 to 1,124,674
_05310
Position (kb)
1120
1121 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1120.608 kb on - strand at 1120.608 kb on - strand at 1120.609 kb on - strand at 1120.611 kb on - strand at 1120.611 kb on - strand at 1120.611 kb on - strand at 1120.638 kb on - strand at 1120.698 kb on + strand, within IAI47_05305 at 1120.698 kb on + strand, within IAI47_05305 at 1120.698 kb on + strand, within IAI47_05305 at 1120.780 kb on - strand, within IAI47_05305 at 1120.803 kb on + strand, within IAI47_05305 at 1120.803 kb on + strand, within IAI47_05305 at 1120.803 kb on + strand, within IAI47_05305 at 1120.803 kb on + strand, within IAI47_05305 at 1120.804 kb on - strand, within IAI47_05305 at 1120.804 kb on - strand, within IAI47_05305 at 1120.804 kb on - strand, within IAI47_05305 at 1120.804 kb on - strand, within IAI47_05305 at 1120.804 kb on - strand, within IAI47_05305 at 1120.804 kb on - strand, within IAI47_05305 at 1120.817 kb on + strand, within IAI47_05305 at 1120.818 kb on - strand, within IAI47_05305 at 1120.875 kb on - strand, within IAI47_05305 at 1120.889 kb on + strand, within IAI47_05305 at 1120.890 kb on - strand, within IAI47_05305 at 1120.930 kb on + strand, within IAI47_05305 at 1120.989 kb on + strand at 1121.038 kb on + strand at 1121.038 kb on + strand at 1121.038 kb on + strand at 1121.039 kb on - strand at 1121.039 kb on - strand at 1121.039 kb on - strand at 1121.039 kb on - strand at 1121.043 kb on + strand at 1121.043 kb on + strand at 1121.044 kb on - strand at 1121.044 kb on - strand at 1121.044 kb on - strand at 1121.197 kb on + strand at 1121.197 kb on + strand at 1121.198 kb on - strand at 1121.202 kb on + strand at 1121.202 kb on + strand at 1121.202 kb on + strand at 1121.203 kb on - strand at 1121.203 kb on - strand at 1121.203 kb on - strand at 1121.203 kb on - strand at 1121.219 kb on + strand at 1121.224 kb on + strand at 1121.225 kb on - strand at 1121.229 kb on + strand at 1121.298 kb on + strand at 1121.318 kb on - strand at 1121.318 kb on - strand at 1121.318 kb on - strand at 1121.328 kb on + strand at 1121.328 kb on + strand at 1121.328 kb on + strand at 1121.328 kb on + strand at 1121.329 kb on - strand at 1121.329 kb on - strand at 1121.329 kb on - strand at 1121.334 kb on + strand at 1121.334 kb on + strand at 1121.335 kb on - strand at 1121.335 kb on - strand at 1121.359 kb on + strand at 1121.376 kb on + strand at 1121.376 kb on + strand at 1121.376 kb on + strand at 1121.376 kb on + strand at 1121.376 kb on + strand at 1121.376 kb on + strand at 1121.376 kb on + strand at 1121.377 kb on - strand at 1121.377 kb on - strand at 1121.377 kb on - strand at 1121.377 kb on - strand at 1121.377 kb on - strand at 1121.439 kb on + strand at 1121.439 kb on + strand at 1121.450 kb on + strand at 1121.450 kb on + strand at 1121.450 kb on + strand at 1121.450 kb on + strand at 1121.451 kb on - strand at 1121.463 kb on + strand at 1121.464 kb on - strand at 1121.464 kb on - strand at 1121.497 kb on - strand at 1121.511 kb on + strand at 1121.511 kb on + strand at 1121.511 kb on + strand at 1121.512 kb on - strand at 1121.543 kb on + strand at 1121.738 kb on - strand at 1121.738 kb on - strand at 1121.819 kb on - strand at 1121.819 kb on - strand at 1121.824 kb on + strand at 1121.904 kb on + strand, within IAI47_05310 at 1121.904 kb on + strand, within IAI47_05310 at 1121.904 kb on + strand, within IAI47_05310 at 1121.904 kb on + strand, within IAI47_05310 at 1121.905 kb on - strand, within IAI47_05310 at 1121.905 kb on - strand, within IAI47_05310
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,120,608 - -2.0 1,120,608 - -1.1 1,120,609 - +0.0 1,120,611 - -4.1 1,120,611 - -2.1 1,120,611 - -1.6 1,120,638 - +1.3 1,120,698 + IAI47_05305 0.24 -2.3 1,120,698 + IAI47_05305 0.24 -2.7 1,120,698 + IAI47_05305 0.24 -1.3 1,120,780 - IAI47_05305 0.46 -0.6 1,120,803 + IAI47_05305 0.53 -2.3 1,120,803 + IAI47_05305 0.53 -0.6 1,120,803 + IAI47_05305 0.53 -1.4 1,120,803 + IAI47_05305 0.53 -1.6 1,120,804 - IAI47_05305 0.53 -0.1 1,120,804 - IAI47_05305 0.53 +1.0 1,120,804 - IAI47_05305 0.53 +0.7 1,120,804 - IAI47_05305 0.53 +1.5 1,120,804 - IAI47_05305 0.53 +1.4 1,120,804 - IAI47_05305 0.53 -2.3 1,120,817 + IAI47_05305 0.56 -2.0 1,120,818 - IAI47_05305 0.57 -1.5 1,120,875 - IAI47_05305 0.72 -1.4 1,120,889 + IAI47_05305 0.76 -1.5 1,120,890 - IAI47_05305 0.76 +0.7 1,120,930 + IAI47_05305 0.87 -1.1 1,120,989 + -2.9 1,121,038 + -1.2 1,121,038 + -0.2 1,121,038 + -0.6 1,121,039 - -0.0 1,121,039 - +2.9 1,121,039 - +4.2 1,121,039 - +0.4 1,121,043 + +1.3 1,121,043 + -1.2 1,121,044 - +3.2 1,121,044 - -0.1 1,121,044 - -0.3 1,121,197 + -2.9 1,121,197 + -0.6 1,121,198 - +0.9 1,121,202 + +1.2 1,121,202 + +1.0 1,121,202 + +1.1 1,121,203 - -1.7 1,121,203 - +0.1 1,121,203 - +0.5 1,121,203 - -0.8 1,121,219 + -0.8 1,121,224 + -1.3 1,121,225 - +0.6 1,121,229 + -0.9 1,121,298 + +0.2 1,121,318 - +0.7 1,121,318 - -2.0 1,121,318 - -0.5 1,121,328 + -1.0 1,121,328 + -1.1 1,121,328 + -0.2 1,121,328 + +0.2 1,121,329 - +0.9 1,121,329 - -1.4 1,121,329 - -0.3 1,121,334 + -0.3 1,121,334 + +0.2 1,121,335 - -0.4 1,121,335 - +1.5 1,121,359 + +1.5 1,121,376 + -3.5 1,121,376 + -0.4 1,121,376 + -2.1 1,121,376 + +2.0 1,121,376 + +1.0 1,121,376 + -0.1 1,121,376 + -1.5 1,121,377 - +0.2 1,121,377 - -0.2 1,121,377 - -2.0 1,121,377 - +1.4 1,121,377 - -0.2 1,121,439 + -0.3 1,121,439 + -0.7 1,121,450 + -0.3 1,121,450 + +2.0 1,121,450 + -0.3 1,121,450 + -1.0 1,121,451 - -0.6 1,121,463 + -0.8 1,121,464 - -2.2 1,121,464 - -0.5 1,121,497 - -1.4 1,121,511 + +2.3 1,121,511 + -1.4 1,121,511 + +3.1 1,121,512 - +0.7 1,121,543 + +0.2 1,121,738 - -1.8 1,121,738 - +0.7 1,121,819 - +1.1 1,121,819 - -0.8 1,121,824 + +1.9 1,121,904 + IAI47_05310 0.11 -0.1 1,121,904 + IAI47_05310 0.11 +0.5 1,121,904 + IAI47_05310 0.11 -0.1 1,121,904 + IAI47_05310 0.11 +1.2 1,121,905 - IAI47_05310 0.11 -0.1 1,121,905 - IAI47_05310 0.11 +0.7
Or see this region's nucleotide sequence