Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25640 and BPHYT_RS25645 are separated by 91 nucleotides BPHYT_RS25645 and BPHYT_RS25650 overlap by 4 nucleotides BPHYT_RS25650 and BPHYT_RS25655 are separated by 111 nucleotides BPHYT_RS25655 and BPHYT_RS25660 are separated by 2 nucleotides
BPHYT_RS25640: BPHYT_RS25640 - MFS transporter, at 1,324,884 to 1,326,218
_RS25640
BPHYT_RS25645: BPHYT_RS25645 - dehydrogenase, at 1,326,310 to 1,327,203
_RS25645
BPHYT_RS25650: BPHYT_RS25650 - dihydroxy-acid dehydratase, at 1,327,200 to 1,328,966
_RS25650
BPHYT_RS25655: BPHYT_RS25655 - short-chain dehydrogenase, at 1,329,078 to 1,329,878
_RS25655
BPHYT_RS25660: BPHYT_RS25660 - MFS transporter, at 1,329,881 to 1,331,239
_RS25660
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-2
-1
0
1 at 1326.244 kb on + strand at 1326.245 kb on - strand at 1326.246 kb on + strand at 1326.433 kb on - strand, within BPHYT_RS25645 at 1327.003 kb on + strand, within BPHYT_RS25645 at 1327.422 kb on - strand, within BPHYT_RS25650 at 1328.205 kb on - strand, within BPHYT_RS25650 at 1328.621 kb on - strand, within BPHYT_RS25650 at 1328.625 kb on - strand, within BPHYT_RS25650 at 1329.505 kb on + strand, within BPHYT_RS25655
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 1,326,244 + -0.4 1,326,245 - +0.3 1,326,246 + +0.6 1,326,433 - BPHYT_RS25645 0.14 +1.1 1,327,003 + BPHYT_RS25645 0.78 +0.6 1,327,422 - BPHYT_RS25650 0.13 -0.6 1,328,205 - BPHYT_RS25650 0.57 -0.4 1,328,621 - BPHYT_RS25650 0.80 -2.0 1,328,625 - BPHYT_RS25650 0.81 -0.4 1,329,505 + BPHYT_RS25655 0.53 -0.7
Or see this region's nucleotide sequence