Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_15230 and IAI47_15235 are separated by 11 nucleotides IAI47_15235 and IAI47_15240 overlap by 4 nucleotides IAI47_15240 and IAI47_15245 are separated by 3 nucleotides IAI47_15245 and IAI47_15250 overlap by 4 nucleotides
IAI47_15230: IAI47_15230 - amidohydrolase, at 3,212,437 to 3,213,204
_15230
IAI47_15235: IAI47_15235 - hydroxyisourate hydrolase, at 3,213,216 to 3,213,551
_15235
IAI47_15240: IAI47_15240 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, at 3,213,548 to 3,214,042
_15240
IAI47_15245: IAI47_15245 - allantoate amidohydrolase, at 3,214,046 to 3,215,299
_15245
IAI47_15250: IAI47_15250 - alanine--glyoxylate aminotransferase family protein, at 3,215,296 to 3,216,531
_15250
Position (kb)
3214
3215
3216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3213.064 kb on - strand, within IAI47_15230 at 3213.064 kb on - strand, within IAI47_15230 at 3213.155 kb on - strand at 3213.202 kb on + strand at 3213.218 kb on - strand at 3213.320 kb on - strand, within IAI47_15235 at 3213.320 kb on - strand, within IAI47_15235 at 3213.320 kb on - strand, within IAI47_15235 at 3213.349 kb on + strand, within IAI47_15235 at 3213.350 kb on - strand, within IAI47_15235 at 3213.350 kb on - strand, within IAI47_15235 at 3213.376 kb on + strand, within IAI47_15235 at 3213.377 kb on - strand, within IAI47_15235 at 3213.377 kb on - strand, within IAI47_15235 at 3213.509 kb on + strand, within IAI47_15235 at 3213.530 kb on + strand at 3213.530 kb on + strand at 3213.530 kb on + strand at 3213.530 kb on + strand at 3213.530 kb on + strand at 3213.531 kb on - strand at 3213.531 kb on - strand at 3213.566 kb on - strand at 3213.572 kb on + strand at 3213.573 kb on - strand at 3213.580 kb on + strand at 3213.581 kb on - strand at 3213.628 kb on + strand, within IAI47_15240 at 3213.629 kb on - strand, within IAI47_15240 at 3213.720 kb on + strand, within IAI47_15240 at 3213.720 kb on + strand, within IAI47_15240 at 3213.720 kb on + strand, within IAI47_15240 at 3213.721 kb on - strand, within IAI47_15240 at 3213.721 kb on - strand, within IAI47_15240 at 3213.721 kb on - strand, within IAI47_15240 at 3213.727 kb on + strand, within IAI47_15240 at 3213.727 kb on + strand, within IAI47_15240 at 3213.727 kb on + strand, within IAI47_15240 at 3213.728 kb on - strand, within IAI47_15240 at 3213.760 kb on - strand, within IAI47_15240 at 3213.760 kb on - strand, within IAI47_15240 at 3213.760 kb on - strand, within IAI47_15240 at 3213.801 kb on - strand, within IAI47_15240 at 3213.801 kb on - strand, within IAI47_15240 at 3213.801 kb on - strand, within IAI47_15240 at 3213.914 kb on + strand, within IAI47_15240 at 3214.024 kb on + strand at 3214.024 kb on + strand at 3214.024 kb on + strand at 3214.025 kb on - strand at 3214.025 kb on - strand at 3214.035 kb on - strand at 3214.035 kb on - strand at 3214.043 kb on - strand at 3214.047 kb on + strand at 3214.047 kb on + strand at 3214.048 kb on - strand at 3214.083 kb on + strand at 3214.083 kb on + strand at 3214.156 kb on + strand at 3214.156 kb on + strand at 3214.156 kb on + strand at 3214.166 kb on + strand at 3214.166 kb on + strand at 3214.166 kb on + strand at 3214.166 kb on + strand at 3214.166 kb on + strand at 3214.166 kb on + strand at 3214.167 kb on - strand at 3214.167 kb on - strand at 3214.167 kb on - strand at 3214.167 kb on - strand at 3214.167 kb on - strand at 3214.167 kb on - strand at 3214.196 kb on - strand, within IAI47_15245 at 3214.237 kb on + strand, within IAI47_15245 at 3214.238 kb on - strand, within IAI47_15245 at 3214.238 kb on - strand, within IAI47_15245 at 3214.238 kb on - strand, within IAI47_15245 at 3214.238 kb on - strand, within IAI47_15245 at 3214.300 kb on + strand, within IAI47_15245 at 3214.301 kb on - strand, within IAI47_15245 at 3214.314 kb on + strand, within IAI47_15245 at 3214.317 kb on + strand, within IAI47_15245 at 3214.318 kb on - strand, within IAI47_15245 at 3214.318 kb on - strand, within IAI47_15245 at 3214.318 kb on - strand, within IAI47_15245 at 3214.374 kb on - strand, within IAI47_15245 at 3214.439 kb on + strand, within IAI47_15245 at 3214.439 kb on + strand, within IAI47_15245 at 3214.439 kb on + strand, within IAI47_15245 at 3214.440 kb on - strand, within IAI47_15245 at 3214.440 kb on - strand, within IAI47_15245 at 3214.480 kb on + strand, within IAI47_15245 at 3214.480 kb on + strand, within IAI47_15245 at 3214.480 kb on + strand, within IAI47_15245 at 3214.481 kb on - strand, within IAI47_15245 at 3214.481 kb on - strand, within IAI47_15245 at 3214.651 kb on + strand, within IAI47_15245 at 3214.725 kb on + strand, within IAI47_15245 at 3214.735 kb on + strand, within IAI47_15245 at 3214.810 kb on + strand, within IAI47_15245 at 3214.810 kb on + strand, within IAI47_15245 at 3214.810 kb on + strand, within IAI47_15245 at 3214.811 kb on - strand, within IAI47_15245 at 3214.811 kb on - strand, within IAI47_15245 at 3214.811 kb on - strand, within IAI47_15245 at 3214.811 kb on - strand, within IAI47_15245 at 3214.886 kb on - strand, within IAI47_15245 at 3214.886 kb on - strand, within IAI47_15245 at 3214.973 kb on + strand, within IAI47_15245 at 3214.973 kb on + strand, within IAI47_15245 at 3214.973 kb on + strand, within IAI47_15245 at 3214.973 kb on + strand, within IAI47_15245 at 3214.973 kb on + strand, within IAI47_15245 at 3215.088 kb on + strand, within IAI47_15245 at 3215.088 kb on + strand, within IAI47_15245 at 3215.101 kb on + strand, within IAI47_15245 at 3215.101 kb on + strand, within IAI47_15245 at 3215.101 kb on + strand, within IAI47_15245 at 3215.102 kb on - strand, within IAI47_15245 at 3215.102 kb on - strand, within IAI47_15245 at 3215.102 kb on - strand, within IAI47_15245 at 3215.193 kb on + strand at 3215.193 kb on + strand at 3215.193 kb on + strand at 3215.193 kb on + strand at 3215.313 kb on - strand at 3215.426 kb on + strand, within IAI47_15250 at 3215.427 kb on - strand, within IAI47_15250 at 3215.743 kb on - strand, within IAI47_15250 at 3215.743 kb on - strand, within IAI47_15250 at 3215.762 kb on + strand, within IAI47_15250 at 3215.762 kb on + strand, within IAI47_15250 at 3215.762 kb on + strand, within IAI47_15250 at 3215.790 kb on - strand, within IAI47_15250 at 3215.790 kb on - strand, within IAI47_15250 at 3215.790 kb on - strand, within IAI47_15250 at 3215.799 kb on - strand, within IAI47_15250 at 3215.799 kb on - strand, within IAI47_15250 at 3215.799 kb on - strand, within IAI47_15250 at 3216.017 kb on + strand, within IAI47_15250 at 3216.017 kb on + strand, within IAI47_15250 at 3216.017 kb on + strand, within IAI47_15250 at 3216.018 kb on - strand, within IAI47_15250 at 3216.032 kb on + strand, within IAI47_15250 at 3216.032 kb on + strand, within IAI47_15250 at 3216.033 kb on - strand, within IAI47_15250 at 3216.039 kb on + strand, within IAI47_15250 at 3216.040 kb on - strand, within IAI47_15250 at 3216.087 kb on + strand, within IAI47_15250 at 3216.157 kb on - strand, within IAI47_15250 at 3216.293 kb on - strand, within IAI47_15250 at 3216.293 kb on - strand, within IAI47_15250 at 3216.293 kb on - strand, within IAI47_15250
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,213,064 - IAI47_15230 0.82 -1.2 3,213,064 - IAI47_15230 0.82 -3.3 3,213,155 - -0.3 3,213,202 + -1.9 3,213,218 - +1.2 3,213,320 - IAI47_15235 0.31 +0.7 3,213,320 - IAI47_15235 0.31 +0.7 3,213,320 - IAI47_15235 0.31 +1.9 3,213,349 + IAI47_15235 0.40 -0.7 3,213,350 - IAI47_15235 0.40 +0.5 3,213,350 - IAI47_15235 0.40 -0.4 3,213,376 + IAI47_15235 0.48 -0.7 3,213,377 - IAI47_15235 0.48 -0.3 3,213,377 - IAI47_15235 0.48 +1.2 3,213,509 + IAI47_15235 0.87 +1.7 3,213,530 + +2.2 3,213,530 + -0.7 3,213,530 + +0.7 3,213,530 + +1.0 3,213,530 + -2.4 3,213,531 - +2.3 3,213,531 - +1.2 3,213,566 - -1.8 3,213,572 + +0.1 3,213,573 - -0.8 3,213,580 + -1.3 3,213,581 - +0.5 3,213,628 + IAI47_15240 0.16 -1.5 3,213,629 - IAI47_15240 0.16 -1.7 3,213,720 + IAI47_15240 0.35 -1.9 3,213,720 + IAI47_15240 0.35 -1.5 3,213,720 + IAI47_15240 0.35 -1.9 3,213,721 - IAI47_15240 0.35 +0.8 3,213,721 - IAI47_15240 0.35 +0.3 3,213,721 - IAI47_15240 0.35 -1.9 3,213,727 + IAI47_15240 0.36 -0.6 3,213,727 + IAI47_15240 0.36 +0.1 3,213,727 + IAI47_15240 0.36 -2.4 3,213,728 - IAI47_15240 0.36 +0.0 3,213,760 - IAI47_15240 0.43 -1.7 3,213,760 - IAI47_15240 0.43 -1.6 3,213,760 - IAI47_15240 0.43 -0.4 3,213,801 - IAI47_15240 0.51 +0.3 3,213,801 - IAI47_15240 0.51 -0.5 3,213,801 - IAI47_15240 0.51 +0.4 3,213,914 + IAI47_15240 0.74 -0.7 3,214,024 + +1.0 3,214,024 + +2.9 3,214,024 + -3.5 3,214,025 - +0.7 3,214,025 - +1.7 3,214,035 - -0.0 3,214,035 - +0.4 3,214,043 - +0.8 3,214,047 + +0.4 3,214,047 + -1.9 3,214,048 - -1.7 3,214,083 + +0.8 3,214,083 + -0.4 3,214,156 + -0.7 3,214,156 + +0.0 3,214,156 + +0.3 3,214,166 + -0.9 3,214,166 + +2.2 3,214,166 + -1.2 3,214,166 + -1.1 3,214,166 + -1.8 3,214,166 + -0.6 3,214,167 - +2.2 3,214,167 - -1.8 3,214,167 - -1.1 3,214,167 - -0.5 3,214,167 - +1.6 3,214,167 - -0.3 3,214,196 - IAI47_15245 0.12 -0.2 3,214,237 + IAI47_15245 0.15 -0.5 3,214,238 - IAI47_15245 0.15 -1.1 3,214,238 - IAI47_15245 0.15 -0.4 3,214,238 - IAI47_15245 0.15 -1.0 3,214,238 - IAI47_15245 0.15 -1.3 3,214,300 + IAI47_15245 0.20 -2.2 3,214,301 - IAI47_15245 0.20 -1.3 3,214,314 + IAI47_15245 0.21 -0.1 3,214,317 + IAI47_15245 0.22 +1.2 3,214,318 - IAI47_15245 0.22 -0.7 3,214,318 - IAI47_15245 0.22 +1.8 3,214,318 - IAI47_15245 0.22 -0.5 3,214,374 - IAI47_15245 0.26 +1.7 3,214,439 + IAI47_15245 0.31 -1.1 3,214,439 + IAI47_15245 0.31 +0.8 3,214,439 + IAI47_15245 0.31 -0.3 3,214,440 - IAI47_15245 0.31 +0.7 3,214,440 - IAI47_15245 0.31 +1.7 3,214,480 + IAI47_15245 0.35 +0.3 3,214,480 + IAI47_15245 0.35 -0.0 3,214,480 + IAI47_15245 0.35 +0.5 3,214,481 - IAI47_15245 0.35 -0.7 3,214,481 - IAI47_15245 0.35 +0.8 3,214,651 + IAI47_15245 0.48 -1.0 3,214,725 + IAI47_15245 0.54 -0.7 3,214,735 + IAI47_15245 0.55 +1.4 3,214,810 + IAI47_15245 0.61 +0.7 3,214,810 + IAI47_15245 0.61 -0.7 3,214,810 + IAI47_15245 0.61 -0.1 3,214,811 - IAI47_15245 0.61 +0.9 3,214,811 - IAI47_15245 0.61 +1.5 3,214,811 - IAI47_15245 0.61 -0.7 3,214,811 - IAI47_15245 0.61 +0.2 3,214,886 - IAI47_15245 0.67 -3.1 3,214,886 - IAI47_15245 0.67 -0.2 3,214,973 + IAI47_15245 0.74 -0.0 3,214,973 + IAI47_15245 0.74 -1.5 3,214,973 + IAI47_15245 0.74 -0.8 3,214,973 + IAI47_15245 0.74 -0.9 3,214,973 + IAI47_15245 0.74 -1.1 3,215,088 + IAI47_15245 0.83 +0.4 3,215,088 + IAI47_15245 0.83 -0.2 3,215,101 + IAI47_15245 0.84 +0.8 3,215,101 + IAI47_15245 0.84 +0.9 3,215,101 + IAI47_15245 0.84 +1.8 3,215,102 - IAI47_15245 0.84 -0.3 3,215,102 - IAI47_15245 0.84 +1.1 3,215,102 - IAI47_15245 0.84 +1.7 3,215,193 + +1.2 3,215,193 + -2.0 3,215,193 + -0.6 3,215,193 + +1.2 3,215,313 - +5.9 3,215,426 + IAI47_15250 0.11 +0.5 3,215,427 - IAI47_15250 0.11 +0.8 3,215,743 - IAI47_15250 0.36 +1.3 3,215,743 - IAI47_15250 0.36 +0.1 3,215,762 + IAI47_15250 0.38 -2.2 3,215,762 + IAI47_15250 0.38 -1.9 3,215,762 + IAI47_15250 0.38 +0.4 3,215,790 - IAI47_15250 0.40 -0.8 3,215,790 - IAI47_15250 0.40 -1.2 3,215,790 - IAI47_15250 0.40 +2.1 3,215,799 - IAI47_15250 0.41 -0.3 3,215,799 - IAI47_15250 0.41 -1.5 3,215,799 - IAI47_15250 0.41 +0.0 3,216,017 + IAI47_15250 0.58 +0.1 3,216,017 + IAI47_15250 0.58 +0.5 3,216,017 + IAI47_15250 0.58 -1.9 3,216,018 - IAI47_15250 0.58 +0.1 3,216,032 + IAI47_15250 0.60 +1.3 3,216,032 + IAI47_15250 0.60 +1.1 3,216,033 - IAI47_15250 0.60 -1.5 3,216,039 + IAI47_15250 0.60 +1.2 3,216,040 - IAI47_15250 0.60 +1.1 3,216,087 + IAI47_15250 0.64 +0.2 3,216,157 - IAI47_15250 0.70 -0.9 3,216,293 - IAI47_15250 0.81 -1.5 3,216,293 - IAI47_15250 0.81 -2.4 3,216,293 - IAI47_15250 0.81 -3.2
Or see this region's nucleotide sequence