Experiment: No vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24995 and BPHYT_RS25000 are separated by 471 nucleotides BPHYT_RS25000 and BPHYT_RS25005 are separated by 38 nucleotides BPHYT_RS25005 and BPHYT_RS25010 are separated by 194 nucleotides
BPHYT_RS24995: BPHYT_RS24995 - hypothetical protein, at 1,176,974 to 1,178,122
_RS24995
BPHYT_RS25000: BPHYT_RS25000 - MFS transporter, at 1,178,594 to 1,179,838
_RS25000
BPHYT_RS25005: BPHYT_RS25005 - FMN reductase, at 1,179,877 to 1,180,422
_RS25005
BPHYT_RS25010: BPHYT_RS25010 - N5,N10-methylene tetrahydromethanopterin reductase, at 1,180,617 to 1,182,008
_RS25010
Position (kb) 1178
1179
1180 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1177.709 kb on - strand, within BPHYT_RS24995 at 1178.581 kb on - strand at 1178.657 kb on - strand at 1178.657 kb on - strand at 1179.309 kb on + strand, within BPHYT_RS25000 at 1179.310 kb on - strand, within BPHYT_RS25000 at 1179.310 kb on - strand, within BPHYT_RS25000 at 1180.002 kb on + strand, within BPHYT_RS25005 at 1180.003 kb on - strand, within BPHYT_RS25005 at 1180.747 kb on + strand at 1180.818 kb on - strand, within BPHYT_RS25010
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t1 remove 1,177,709 - BPHYT_RS24995 0.64 +0.5 1,178,581 - -1.8 1,178,657 - +2.8 1,178,657 - +0.7 1,179,309 + BPHYT_RS25000 0.57 +1.0 1,179,310 - BPHYT_RS25000 0.58 +1.7 1,179,310 - BPHYT_RS25000 0.58 +0.5 1,180,002 + BPHYT_RS25005 0.23 -0.7 1,180,003 - BPHYT_RS25005 0.23 -2.5 1,180,747 + -0.2 1,180,818 - BPHYT_RS25010 0.14 +0.0
Or see this region's nucleotide sequence