Experiment: With vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34970 and BPHYT_RS34975 overlap by 8 nucleotides BPHYT_RS34975 and BPHYT_RS34980 are separated by 40 nucleotides BPHYT_RS34980 and BPHYT_RS34985 are separated by 26 nucleotides BPHYT_RS34985 and BPHYT_RS34990 overlap by 8 nucleotides
BPHYT_RS34970: BPHYT_RS34970 - hypothetical protein, at 3,444,385 to 3,446,043
_RS34970
BPHYT_RS34975: BPHYT_RS34975 - L-aspartate dehydrogenase, at 3,446,036 to 3,446,866
_RS34975
BPHYT_RS34980: BPHYT_RS34980 - aldehyde dehydrogenase, at 3,446,907 to 3,448,397
_RS34980
BPHYT_RS34985: BPHYT_RS34985 - glyoxalase, at 3,448,424 to 3,449,389
_RS34985
BPHYT_RS34990: BPHYT_RS34990 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,449,382 to 3,450,647
_RS34990
Position (kb)
3446
3447
3448
3449 Strain fitness (log2 ratio)
-2
-1
0
1 at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.199 kb on + strand, within BPHYT_RS34975 at 3446.493 kb on - strand, within BPHYT_RS34975 at 3446.493 kb on - strand, within BPHYT_RS34975 at 3446.865 kb on - strand at 3447.368 kb on + strand, within BPHYT_RS34980 at 3447.368 kb on + strand, within BPHYT_RS34980 at 3447.745 kb on - strand, within BPHYT_RS34980 at 3448.021 kb on - strand, within BPHYT_RS34980 at 3448.031 kb on + strand, within BPHYT_RS34980 at 3448.189 kb on - strand, within BPHYT_RS34980 at 3448.335 kb on + strand at 3448.395 kb on + strand at 3448.854 kb on - strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t2 remove 3,446,118 - -0.4 3,446,118 - +0.5 3,446,118 - -0.4 3,446,118 - +0.2 3,446,199 + BPHYT_RS34975 0.20 -1.4 3,446,493 - BPHYT_RS34975 0.55 -0.5 3,446,493 - BPHYT_RS34975 0.55 -1.4 3,446,865 - -0.1 3,447,368 + BPHYT_RS34980 0.31 -0.5 3,447,368 + BPHYT_RS34980 0.31 +1.2 3,447,745 - BPHYT_RS34980 0.56 -1.8 3,448,021 - BPHYT_RS34980 0.75 +0.0 3,448,031 + BPHYT_RS34980 0.75 +0.4 3,448,189 - BPHYT_RS34980 0.86 -0.8 3,448,335 + -1.0 3,448,395 + -0.4 3,448,854 - BPHYT_RS34985 0.45 -0.6 3,449,113 + BPHYT_RS34985 0.71 -0.2 3,449,113 + BPHYT_RS34985 0.71 -0.5
Or see this region's nucleotide sequence