Experiment: With vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04730 and BPHYT_RS04735 overlap by 4 nucleotides BPHYT_RS04735 and BPHYT_RS04740 are separated by 110 nucleotides BPHYT_RS04740 and BPHYT_RS04745 are separated by 25 nucleotides
BPHYT_RS04730: BPHYT_RS04730 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 1,093,713 to 1,094,252
_RS04730
BPHYT_RS04735: BPHYT_RS04735 - phosphoadenosine phosphosulfate reductase, at 1,094,249 to 1,094,995
_RS04735
BPHYT_RS04740: BPHYT_RS04740 - sulfate adenylyltransferase subunit 2, at 1,095,106 to 1,096,068
_RS04740
BPHYT_RS04745: BPHYT_RS04745 - sulfate adenylyltransferase, at 1,096,094 to 1,097,410
_RS04745
Position (kb)
1095
1096
1097 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1094.178 kb on + strand, within BPHYT_RS04730 at 1095.105 kb on + strand at 1095.106 kb on - strand at 1095.348 kb on + strand, within BPHYT_RS04740 at 1095.367 kb on + strand, within BPHYT_RS04740 at 1095.368 kb on - strand, within BPHYT_RS04740 at 1095.748 kb on + strand, within BPHYT_RS04740 at 1095.748 kb on + strand, within BPHYT_RS04740 at 1095.749 kb on - strand, within BPHYT_RS04740 at 1095.749 kb on - strand, within BPHYT_RS04740 at 1095.787 kb on + strand, within BPHYT_RS04740 at 1095.787 kb on + strand, within BPHYT_RS04740 at 1095.912 kb on + strand, within BPHYT_RS04740 at 1095.913 kb on - strand, within BPHYT_RS04740 at 1096.066 kb on + strand at 1096.193 kb on + strand at 1096.194 kb on - strand at 1096.256 kb on - strand, within BPHYT_RS04745 at 1096.403 kb on + strand, within BPHYT_RS04745 at 1096.593 kb on - strand, within BPHYT_RS04745 at 1096.628 kb on + strand, within BPHYT_RS04745 at 1096.862 kb on + strand, within BPHYT_RS04745
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t2 remove 1,094,178 + BPHYT_RS04730 0.86 -2.9 1,095,105 + -2.9 1,095,106 - -2.5 1,095,348 + BPHYT_RS04740 0.25 -6.0 1,095,367 + BPHYT_RS04740 0.27 -0.9 1,095,368 - BPHYT_RS04740 0.27 -0.9 1,095,748 + BPHYT_RS04740 0.67 -2.2 1,095,748 + BPHYT_RS04740 0.67 -2.7 1,095,749 - BPHYT_RS04740 0.67 -2.2 1,095,749 - BPHYT_RS04740 0.67 -2.5 1,095,787 + BPHYT_RS04740 0.71 -3.6 1,095,787 + BPHYT_RS04740 0.71 -1.9 1,095,912 + BPHYT_RS04740 0.84 -4.1 1,095,913 - BPHYT_RS04740 0.84 -3.0 1,096,066 + -2.5 1,096,193 + -1.5 1,096,194 - -2.5 1,096,256 - BPHYT_RS04745 0.12 +0.1 1,096,403 + BPHYT_RS04745 0.23 -4.3 1,096,593 - BPHYT_RS04745 0.38 -1.5 1,096,628 + BPHYT_RS04745 0.41 -0.9 1,096,862 + BPHYT_RS04745 0.58 -2.9
Or see this region's nucleotide sequence