Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0082 and Dshi_0083 are separated by 105 nucleotides Dshi_0083 and Dshi_0084 are separated by 37 nucleotides Dshi_0084 and Dshi_0085 are separated by 34 nucleotides
Dshi_0082: Dshi_0082 - hypothetical protein (RefSeq), at 81,262 to 82,386
_0082
Dshi_0083: Dshi_0083 - 3-isopropylmalate dehydratase, small subunit (RefSeq), at 82,492 to 83,097
_0083
Dshi_0084: Dshi_0084 - hypothetical protein (RefSeq), at 83,135 to 83,662
_0084
Dshi_0085: Dshi_0085 - 3-isopropylmalate dehydratase, large subunit (RefSeq), at 83,697 to 85,100
_0085
Position (kb)
83
84 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 82.143 kb on - strand, within Dshi_0082 at 82.170 kb on - strand, within Dshi_0082 at 82.260 kb on + strand, within Dshi_0082 at 82.260 kb on + strand, within Dshi_0082 at 82.260 kb on + strand, within Dshi_0082 at 82.262 kb on - strand, within Dshi_0082 at 82.345 kb on + strand at 82.350 kb on + strand at 82.353 kb on - strand at 82.455 kb on + strand at 82.516 kb on + strand at 82.516 kb on + strand at 82.524 kb on - strand at 82.551 kb on + strand at 82.559 kb on - strand, within Dshi_0083 at 82.559 kb on - strand, within Dshi_0083 at 83.016 kb on + strand, within Dshi_0083 at 83.167 kb on - strand at 83.191 kb on - strand, within Dshi_0084 at 83.213 kb on + strand, within Dshi_0084 at 83.213 kb on - strand, within Dshi_0084 at 83.213 kb on - strand, within Dshi_0084 at 83.330 kb on - strand, within Dshi_0084 at 83.341 kb on + strand, within Dshi_0084 at 83.461 kb on - strand, within Dshi_0084 at 83.473 kb on + strand, within Dshi_0084 at 83.567 kb on + strand, within Dshi_0084 at 83.688 kb on - strand at 84.190 kb on - strand, within Dshi_0085 at 84.339 kb on + strand, within Dshi_0085
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 82,143 - Dshi_0082 0.78 +0.4 82,170 - Dshi_0082 0.81 -0.4 82,260 + Dshi_0082 0.89 -0.1 82,260 + Dshi_0082 0.89 -0.2 82,260 + Dshi_0082 0.89 +0.6 82,262 - Dshi_0082 0.89 +0.4 82,345 + +2.1 82,350 + -0.4 82,353 - -1.0 82,455 + -0.7 82,516 + +0.4 82,516 + -0.2 82,524 - -2.6 82,551 + -2.9 82,559 - Dshi_0083 0.11 -2.0 82,559 - Dshi_0083 0.11 -3.2 83,016 + Dshi_0083 0.86 -1.9 83,167 - -1.6 83,191 - Dshi_0084 0.11 -0.9 83,213 + Dshi_0084 0.15 +1.1 83,213 - Dshi_0084 0.15 -0.2 83,213 - Dshi_0084 0.15 -0.2 83,330 - Dshi_0084 0.37 -0.3 83,341 + Dshi_0084 0.39 -0.4 83,461 - Dshi_0084 0.62 -1.7 83,473 + Dshi_0084 0.64 +1.1 83,567 + Dshi_0084 0.82 -0.6 83,688 - +0.4 84,190 - Dshi_0085 0.35 -2.2 84,339 + Dshi_0085 0.46 -3.4
Or see this region's nucleotide sequence