Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_680 and Pf6N2E2_681 overlap by 4 nucleotides Pf6N2E2_681 and Pf6N2E2_682 are separated by 112 nucleotides Pf6N2E2_682 and Pf6N2E2_683 are separated by 203 nucleotides
Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835
_680
Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764
_681
Pf6N2E2_682 - Predicted regulator PutR for proline utilization, GntR family, at 786,877 to 787,548
_682
Pf6N2E2_683 - Probable MFS transporter, at 787,752 to 788,918
_683
Position (kb) 785
786
787 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.397 kb on - strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.523 kb on - strand, within Pf6N2E2_680 at 785.947 kb on + strand, within Pf6N2E2_681 at 785.947 kb on + strand, within Pf6N2E2_681 at 786.073 kb on + strand, within Pf6N2E2_681 at 786.074 kb on - strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.198 kb on + strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.211 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.408 kb on + strand, within Pf6N2E2_681 at 786.832 kb on - strand at 786.832 kb on - strand at 786.837 kb on + strand at 786.837 kb on + strand at 786.838 kb on - strand at 786.838 kb on - strand at 786.838 kb on - strand at 787.115 kb on + strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.246 kb on + strand, within Pf6N2E2_682 at 787.247 kb on - strand, within Pf6N2E2_682 at 787.585 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 785,092 + Pf6N2E2_680 0.33 -0.8 785,092 + Pf6N2E2_680 0.33 -0.2 785,092 + Pf6N2E2_680 0.33 +0.1 785,092 + Pf6N2E2_680 0.33 +0.2 785,092 + Pf6N2E2_680 0.33 -0.4 785,093 - Pf6N2E2_680 0.33 -0.6 785,093 - Pf6N2E2_680 0.33 -0.5 785,093 - Pf6N2E2_680 0.33 +1.1 785,093 - Pf6N2E2_680 0.33 +1.4 785,093 - Pf6N2E2_680 0.33 -1.2 785,093 - Pf6N2E2_680 0.33 -0.7 785,093 - Pf6N2E2_680 0.33 -1.4 785,094 + Pf6N2E2_680 0.34 -1.6 785,094 + Pf6N2E2_680 0.34 -0.3 785,095 - Pf6N2E2_680 0.34 +0.6 785,095 - Pf6N2E2_680 0.34 -1.5 785,142 - Pf6N2E2_680 0.38 -0.4 785,142 - Pf6N2E2_680 0.38 -2.2 785,397 - Pf6N2E2_680 0.61 -0.7 785,522 + Pf6N2E2_680 0.72 +1.8 785,522 + Pf6N2E2_680 0.72 +0.2 785,523 - Pf6N2E2_680 0.72 -0.8 785,947 + Pf6N2E2_681 0.12 +0.2 785,947 + Pf6N2E2_681 0.12 +0.2 786,073 + Pf6N2E2_681 0.26 +0.8 786,074 - Pf6N2E2_681 0.26 +0.8 786,093 + Pf6N2E2_681 0.28 +0.9 786,093 + Pf6N2E2_681 0.28 +0.0 786,198 + Pf6N2E2_681 0.39 +0.5 786,206 - Pf6N2E2_681 0.40 +0.7 786,206 - Pf6N2E2_681 0.40 -1.0 786,206 - Pf6N2E2_681 0.40 +1.2 786,211 + Pf6N2E2_681 0.41 +0.8 786,355 + Pf6N2E2_681 0.56 +0.7 786,355 + Pf6N2E2_681 0.56 +0.9 786,408 + Pf6N2E2_681 0.62 +0.8 786,832 - +1.3 786,832 - +0.1 786,837 + +0.5 786,837 + -0.4 786,838 - +0.8 786,838 - -0.2 786,838 - +0.0 787,115 + Pf6N2E2_682 0.35 +0.7 787,116 - Pf6N2E2_682 0.36 +0.2 787,116 - Pf6N2E2_682 0.36 -0.2 787,116 - Pf6N2E2_682 0.36 -3.0 787,160 - Pf6N2E2_682 0.42 +0.8 787,160 - Pf6N2E2_682 0.42 -1.1 787,160 - Pf6N2E2_682 0.42 -0.2 787,160 - Pf6N2E2_682 0.42 +1.0 787,246 + Pf6N2E2_682 0.55 -1.2 787,247 - Pf6N2E2_682 0.55 +0.9 787,585 + +0.7
Or see this region's nucleotide sequence